Contact: Steven Clemens
Location: Geological Sciences »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Mass Spectrometer, Isotope Ratio Contact: Steven Clemens, Daniel Ibarra https://sites.brown.edu/mat235-mass-spectrometry/ | ThermoFisher (Finnigan) 253+ dual inlet Isotope Ratio Mass Spectrometer with automated sample reaction device for up to 48 samples and with Kiel IV Carbonate interface and PoraPak purification trap. | Instrument primarily dedicated to the measurement of the stable isotopes of carbon and oxygen on inorganic carbonates as well as clumped oxygen isotopes. The facility accommodates projects both internal and external to Brown including analysis of terrestrial and marine material. The vast majority of samples analyzed are funded through external grants but we also facilitate a significant number of small unfunded, exploratory, analyses to help construct proof-of-concept data sets in preparation for proposals. |
Contact: Patricia Coulter
Location: Advance-CTR Headquarters »See on the MapAffiliated Centers: IDeA Advance RI-CTR (Clinical Translational Research) at Brown University
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Administration Advance-CTR Contact: Research Resources Request a Service | Clinical Research Service, Advance-CTR Service Coordination | Advance-CTR works to support researchers at the state level by developing the infrastructure and resources investigators need to conduct clinical and translational research in Rhode Island. Through its interdisciplinary and inter-institutional model, Advance-CTR funds and supports research across the translational science spectrum, including basic science, clinical, and public health efforts. Ultimately, Advance-CTR seeks to connect researchers and support institutions in Rhode Island in order to advance clinical and translational research that improves public health in the state. |
Research - Biostatistical Consulting, Epidemiology & Clinical Research Design Services Contact: Heather Fournier Biostatistical Consulting, Epidemiology & Clinical Research Design Services Webpage Request a Service | Clinical Research Service, Advance-CTR | A team of experienced statisticians and methodological experts are on-hand to help investigators prepare their proposals pre-award, and plan their projects upon receipt of funding. Pre-award services include, positioning of specific aims and hypotheses, measure selection, power analysis and data analysis planning. Post-award services include decision support services, data acquisition and management and consultations to assess the impact of potential design changes on final statistical influence. Data analysis and results interpretation services include implementing data analysis plans, statistical analysis consultations, selecting and accessing statistical analysis software, and coaching to best utilize statistical analysis software. Manuscript and grant proposal writing services include assistance with write-ups, data visualizations, integration of interpretations in the broader context of the project, and addressing comments from peer reviewers. |
Research - Biomedical Informatics Services Contact: Heather Fournier Biomedical Informatics Services Webpage Request a Service | Clinical Research Service, Advance-CTR | Researchers may request, access, and use data available through the Unified Research data Sharing Access (URSA) infrastructure. The data include information originating from electronic health data systems at Lifespan, Care New England, and the Rhode Island Quality Institute, as well as from publicly accessible biological and health data sets. |
Research - Qualitative Methods Services Contact: Heather Fournier Qualitative Services Webpage Request a Service | Clinical Research Service, Advance-CTR | This service provides qualitative and mixed methods and data analysis design research support. Qualitative and mixed methods support includes; study design and preparation, original measure development, coaching on qualitative methods design decisions (focus groups, interviews, cognitive interviews), NVivo qualitative software training, help with grant writing of qualitative methods and analysis sections, manuscript and grant proposal review, post interview/focus group debriefing. Data analysis design support includes applied thematic analysis and framework matrix analysis. |
Research - Community Engagement & Outreach Core Community Engagement & Outreach Core Request a Service | Clinical Research Service, Advance-CTR | The CEO Core helps facilitate research endeavors that are relevant, accessible, and impactful to the entire state of Rhode Island. |
Contact: Jonathan Drake, MD
Location: Rhode Island Hospital, Ambulatory Patient Center (APC Building) »See on the MapAffiliated Centers: Alzheimer's Disease & Memory Disorders Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Alzheimer's Disease & Memory Disorders Center Contact: Alzheimer's Disease and Memory Disorders Center | Clinical Research Resources, Alzheimer's | The Rhode Island Hospital’s Alzheimer's Disease and Memory Disorders Center (ADMDC) provides a comprehensive and integrated program of research and education focused on brain aging and dementia. The ADMDC participates in multicenter clinical trials of investigational drugs to prevent or treat the symptoms of Alzheimer’s Disease and is a primary site for studies sponsored by the Alzheimer's Disease Cooperative Study, funded by the National Institutes of Health, and the Alzheimer's Therapeutic Research Institute. Investigator-initiated studies are an important component of the ADMDC’s research activities; the Center is currently conducting innovative clinical trials for cognitively impaired older drivers and mind-body intervention, as well as studies of the roles of neuroimaging, biologic, and cognitive biomarkers in understanding the preclinical and prodromal stages of Alzheimer's disease. A major thrust of current and future dementia research is primary and secondary prevention. The ADMDC is the primary site for the Rhode Island Alzheimer Prevention Registry, a statewide project that enrolls individuals interested in Alzheimer’s disease prevention trials. To further research into the pathology of Alzheimer’s disease and related dementias, the Center supports the brain donation program at the Brown Brain Tissue Resource Center and operates the Biospecimen Bank, a repository for blood and cerebrospinal fluid specimens stored for future collaborative translational research projects. Intra- and inter-institutional research collaborations are a vital part of the ADMDC’s work. The Center hosts a monthly research seminar co-sponsored by the Norman Prince Neurosciences Institute, which provides a forum for aging and dementia researchers to discuss current research and plan future projects. |
Contact: Gerard Nau, MD
Location: Aldrich Building Room 723 The Miriam HospitalAffiliated Centers: IDeA COBRE Center for Antimicrobial Resistance and Therapeutic Discovery (CARTD) at Miriam Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Support for in-vivo experiments | Service | Personnel available to assist with planning and executing BSL1 and BSL2 animal experiments with an emphasis on blinding treatments and/or assessments to enhance robustness. |
Imaging System - fluorescent and bioluminescent | PerkinElmer IVIS Lumina III in vivo imaging system | The IVIS Lumina III is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 26 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral unmixing can be achieved by Lumina III’s optional high resolution short cut off filters. |
Molecular Devices Spectramax i3X Contact: Gerard Nau COBRE CARTD Request a Service | Molecular Devices | The spectramax i3X has the ability to measure absorbance, fluorescence, and luminescence. Additional features such as injectors facilitate flash based applications including dual luciferase and ATP assays. |
Flow Cytometer | Amnis CellStream Luminex Flow Cytometer | The CellStream flow cytometer has single tube and 96 well plate capabilities with automatic compensation. Equipped with 405nm, 488nm, 532nm, and 642nm lasers and 16 fluorescent channels. Handles BSL-1 and fixed samples from BSL-2 experiments. |
Liquid nitrogen storage | CBS Cryosystems 4000 Series | CBS cryosystems 4000 series liquid nitrogen freezer for eukaryotic cell storage. |
PCR Thermal Cycler - Real-Time Detection System | Bio-Rad CFX Connect Real-Time PCR Detection System | CFX Connect Real-Time PCR System for two-target multiplex PCR and routine detection of single fluorophore experiments. |
Neutralization assay | Service | Luciferase-based assay used to measure the neutralization properties of serum, plasma, or monoclonal antibodies against SARS-CoV-2 based on a pseudotyped lentivirus. |
PCR Thermal Cycler - Droplet Digital | Bio-Rad QX200 Droplet Digital PCR System | Absolute quantification of target DNA or RNA molecules using EvaGreen or probe-based digital PCR applications. Flexible platform in groups of 8 with no standard curves necessary. |
Microplate Reader | Molecular Devices Spectramax i3X | The Spectramax i3X has the ability to measure absorbance, fluorescence, and luminescence. Additional features include fluorescence polarization and time-resolved fluorescence, along with injectors that facilitate flash based applications including dual luciferase and ATP assays. |
Biospecimen Banking - Freezer - -80 degrees | PHCbi | PHCbi TwinGuard Series upright -80°C freezer for sample storage. Two independent refrigeration systems provides redundancy for sample protection. |
Contact: Michael Worden
Affiliated Centers: Carney Institute for Brain Science, IDeA COBRE Center for Central Nervous System Function at Brown University
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance - Support | Clinical Research Resources, Behavior and Neuroimaging | The mission of the Behavior and Neuroimaging Core (BNC) is to provide a coherent set of resources that will facilitate the implementation of each of the COBRE Project Leader’s research aims. The BNC provides ongoing support, training, assistance and advice to COBRE project leaders in the practical aspects of data collection, data management and data processing across a wide variety of neuroscience related methodologies. The BNC also serves as a resource to the broader neuroscience community at Brown and its affiliated hospitals and the broader Rhode Island academic community by providing formal and informal training, accessible data processing pipelines, and documentation of these and related resources. The support provided by the BNC is distinct from but complementary to services provided by the Design and Analysis Core (DAC). Whereas the DAC is focused primarily on study design, analytical modeling, and techniques for inference, the focus of the BNC is on practical aspects of research conduct such as efficient and high-quality data acquisition, implementation and deployment of standard and newly developed tools for analysis of large neuroimaging datasets, and lowering barriers to the use of such tools through direct assistance, training and documentation. Because the missions of the BNC and DAC are highly complementary, the effectiveness of each core is strengthened by collaborative relationships between them. The analytical techniques developed by the DAC in partnership with the Project Leaders are predicated upon robust high-quality data. Similarly, realization of study design principles requires practical expertise with the tools necessary to implement those principles. The BNC and DAC will share a full-time staff member with expertise in research design, statistical modeling, data management and high-performance computing. |
Training and Technical Assistance - Facilitate Experimental Implementations | Clinical Research Resources, Behavior and Neuroimaging | Control experiments, Eye tracking, EEG guidance |
Training and Technical Assistance - MRI Assistance | Clinical Research Resources, Behavior and Neuroimaging | Design of experiments, Analysis pipelines, EEG in the MRI scanner, Timing verification |
Data Storage - Generalized Solutions | Clinical Research Resources, Behavior and Neuroimaging | xNAT database, Containers for data presentation, Beyond the desktop with VMs |
Training and Technical Assistance - High Performance Computing (HPC) | Clinical Research Resources, Behavior and Neuroimaging | Assistance with High Performance Computing (HPC) |
Contact: Ivana Delalle, MD, PhD
Location: Rhode Island Hospital Ambulatory Patient Center (APC) 12 »See on the MapAffiliated Centers: Alzheimer's Disease & Memory Disorders Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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BioBank - Brain Tissue Resource Center | Clinical Research Resources, Brain Tissues | A brain tissue resource center (BTRC) (often referred to as a brain bank) is a collection of human tissue specimens from the nervous system: the brain, spinal cord, muscle and peripheral nerves. The facility is engaged in a variety of functions; rapid specimen transport, tissue processing, state-of-the-art preservation methods and database management. A variety of experts, research doctors, neuropathologists and laboratory specialists, plan and manage the BTRC. Strict protocol is imposed on outside vendors whenever their services are required, particularly with respect to rapid specimen transport. |
Contact: AdvanceRI@brown.edu
Location: Lifespan Clinical Research CenterAffiliated Centers: IDeA Advance RI-CTR (Clinical Translational Research) at Brown University, Lifespan Clinical Research Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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BioBank - Covid-19 Biospecimen Banking Biobank – Covid -19 Biospecimen Banking | Service - Covid -19 BioBank | The biobank includes the following samples: Plasma (0.5mLs); LI plasma (0.5mLs); Serum (0.5mLs); PBMCs (0.25mLs / ~2.5M cells) |
Contact: Garrett Sullivan
Location: BioMedical Center Building »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Bioinformatics Analysis - Freezerworks | Software: Freezerworks 2019 Ver 11.01.00 | Sample management software |
BioBank - Biospecimen Banking | Service | Service to store biospecimens at -80 degrees, 4 degrees and in liquid nitrogen. All equipment is connected to back-up generator power and monitored 24/7 by a Rees alarm system. |
BioBank - Biospecimen Banking - Freezer - Liquid Nitrogen | Taylor Wharton LABS 40K Liquid Nitrogen Storage System | (2) Liquid nitrogen biospecimen storage system. Holds up to 40,000 1ml samples |
BioBank - Biospecimen Banking - Freezer - -80 degrees | Thermo Scientific HERAfreezer 24 cu. Ft. -80 degree freezer | (3) Biospecimen Storage at -80 degrees. Holds up to 50,000 1ml samples |
BioBank - Biospecimen Banking - Refrigerator | LABREPCO FUTURA Platinum Series 52 cu. ft. glass door refrigerator | (3) Biospecimen Storage Refrigerators at 4 degrees. 4 degree hinged glass door refrigerator |
BioBank - Robotic Workstation - Sample Preparation | Qiagen QiaCube | DNA, RNA or Protein Purification |
Contact: Braden Fleming, PhD
Location: CORO Center West, Suite 404 »See on the MapAffiliated Centers: IDeA COBRE Center for Skeletal Health and Repair at Rhode Island Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analysis System - Gait | Noldus | Catwlak small animal system |
Analysis System - Force Plate | Kistler | 5 KN portable (6 channel) |
Electromyography (EMG) monitor | Noraxon | Wireless EMG system (8 channel) |
Testing Systems | Bose Corp Biaxial ELF 3200 material test system | Force Testers (Tension, Compression, Pull Testers). The ELF 3200 Series perform basic materials tests including axial, axial/torsion, fretting, biaxial, and planar biaxial fatigue tests. |
Testing Systems | Instron Electropuls E1000 | All-Electric Dynamic Test Instrument designed for dynamic and static testing on a wide range of materials and components. |
Testing Systems | Instron 8521S | Axial-Torsional Fatigue Testing System |
Testing Systems | MTS Bionix Electromechanical Torsion System | Torsion Test System |
Testing Systems | Instron E10000LT | Biaxial material test system |
Microscope | Asylum Research MFP-3D-BIO | Atomic Force Microscope |
Microscope | Nikon TiU inverted microscope | Inverted Microscope |
Imaging System - Camera | Qualisys Oqus 5-Series | The Oqus cameras are designed to capture accurate mocap data with very low latency and works with both passive and active markers. |
Analyzer - Motion | Northern Digital Optotrak System | Track and analyze kinetics and dynamic motion in real-time |
Analysis System - Force Plate | Tekscan Force Plate Walkway | Low profile floor walkway system that captures multiple sequential foot strikes of animals. |
Analyzer - Pressure | Tekscan I-scan | Pressure Mapping System |
Analysis System - Mapping System | Fuji Film Prescale Pressuregraph (FPD-901E) | Pressure Distribution Mapping System |
Pendulum | Custom Active pendulum (small animal joint) | System to induce frictional wear in arthrodial joints |
Pendulum | Custom Passive pendulums (small and large animal joints) | System to measure the whole joint coefficient of friction |
Loading Device | Custom Biaxial Loading Device | Tissue Engineering |
Tissue Engineering System | ICCT Technologies Bio-Stretch Uniaxial System | Mechanical Stretching for Tissue Engineering |
Analysis System - Gait | Tekscan High Resolution Walkway (HRV6) | Modular pressure measurement platform for gait analysis. |
Testing Equipment | KUKA KR6 700 SIXX | 6 DOF joint motion testing |
Contact: Christopher Hemme, PhD
Location: Pharmacy Building »See on the MapAffiliated Centers: IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Bioinformatics Analysis Contact: Christopher Hemme Bioinformatics Core Contact Form | Service | We provide bioinformatics consultation and services to the Rhode Island INBRE community and to the general scientific community of Rhode Island. In addition to traditional bioinformatic services, our Core is expanding to 3D/VR/AR visualization services in conjunction with the URI 3D Visualization Laboratory. We are currently developing 3D/VR visualization apps for a variety of platforms including smartphones, Vive/SteamVR, and others. We also have access to the 3D printer at the URI 3D Visualization Core for printing of marcomolecules at a reasonable price. |
Contact: Lincoln Cox
Location: Arnold Lab »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Contact: Liz Chen, PhD
Affiliated Centers: IDeA Advance RI-CTR (Clinical Translational Research) at Brown University, IDeA COBRE Center for Computational Biology of Human Disease at Brown University
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Biomedical Informatics Services Request a Service | Clinical Research Resources, Biomedical Informatics | Data Requests Storage and management Processing and analysis Submission and dissemination Software and Applications Requests Development REDCap Studies/Projects Project setup Education 5x5 Clinical Informatics elective Biomedical Informatics Learning Series Grant Support |
Biomedical Informatics Resources | Clinical Research Resources, Biomedical Informatics | Cohort Identification i2b2 tranSMART [local] i2b2/SHRINE [regional] TriNetX [national] Data Collection and Management Statewide REDCap instance Wrike and SPARCRequest Data Access and Use Unified Data Sharing & Analysis (URSA) Initiative URSA stronghold URSA data requests URSA governance URSA computational tools Subject Recruitment Research Networking and Resources |
Contact: Evgeny Yakirevich
Location: Lifespan-The Miriam HospitalAffiliated Centers: IDeA COBRE Center for Antimicrobial Resistance and Therapeutic Discovery (CARTD) at Miriam Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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BioBank - Biospecimen Banking - Resource Center Contact: Tao Hong | Clinical Research Resources, Procurement, storage, tracking and release of clinical isolates | Central location of stocked well-characterized clinical isolates to be used for basic, translational and or applied research studies. Over 600 organisms collected to-date. |
Profiling - Microorganism Resistance profiling Contact: Tao Hong | Clinical Research resources, Profiling | Confirm phenotypic resistance profile. Phenotypic susceptibility by broth microdilution, gradient diffusion. Sensititre commercial BMD trays |
Analysis - Microorganism Clinical isolates Contact: Tao Hong | Genotypic identification of isolates | Verify and confirm microorganism identification. 16s ribosomal sequencing, rRNA internal transcribed spacer (ITS) regions. |
DNA Sequencer Contact: Tao Hong | Illumina iseq 100 Illumina assay instrument FS10001514 | Genotypic profiling by whole genome sequencing 16S metagenomic sequencing. Antimicrobial resistance detection by gene sequencing |
BioBank - Biospecimen Banking - Freezer -80 degrees Contact: Tao Hong | Thermo Fisher Scientific REVCO RDE60086FA | Clinical isolates stored at -80 degrees freezer in 1ml glycerol stock tubes |
Contact: Gregory K. Fritz, MD
Location: Bradley Hospital »See on the MapAffiliated Centers: Bradley Hasbro Children's Research Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Child Development and Mental Health Research Contact: Gregory K. Fritz, MD Child Development and Mental Health Research | Clinical Research Resources, Child Development | The Bradley Hasbro Children's Research Center (BHCRC) encompasses a broad spectrum of research programs that share a commitment to studying the impact of psychological factors on the growth and development of children and their families. BHCRC investigators are exploring new insights into the genetic roots of autism; finding pediatric bio-behavioral markers of bipolar disorder; creating effective therapies for OCD; devising effective prevention strategies for adolescent sexual risk behaviors and obesity; and much more. Areas of interest include child and adolescent psychiatric disorders, forensic issues and juvenile justice, pediatric behavioral health, biological basis of psychiatric disorders, prevention and early intervention, and health services research. |
Research - Pediatric Mood, Imaging and Neurodevelopment (Pedi-MIND) program Contact: Daniel Dickstein, MD Pediatric Mood, Imaging and Neurodevelopment (Pedi-MIND) program | Clinical Research Service, Pediatric Imaging | The goal of the Pediatric Mood, Imaging and Neurodevelopment (Pedi-MIND) program is to identify biological and behavioral markers of psychiatric illness in children and adolescents in order to improve diagnosis, treatment, and prevention of these conditions. |
Research - Rhode Island Consortium for Autism Research & Treatment (RI-CART) Contact: RICART RI-CART Website | Clinical Research Resources, Autism | The Rhode Island Consortium for Autism Research & Treatment (RI-CART) is working to improve the lives of Rhode Islanders with autism spectrum disorder and related neurodevelopmental disorders by promoting collaborative, cutting-edge research, improving health care and support services, and providing data-driven education and advocacy. We’re a community of families and individuals, researchers, clinicians, educators, and local agencies focused on both today’s and future generations. |
Research - Bradley Hospital Sleep and Chronobiology Research Laboratory (Sleep Lab) Contact: Mary A. Carskadon, MD Sleep for Science (Sleep Research Lab) Website | Clinical Research Resources, Sleep | Sleep for Science is a program of research focusing on understanding how sleep patterns are shaped and how they affect other activities, functions, and biological processes. Our research is chiefly about sleep patterns and processes in young humans – children, teens, and young adults. |
Research - Developmental Disorders Genetics Research Contact: Eric Morrow DDGRP Website | Clinical Research Resources, Developmental Genetics | The Developmental Disorders Genetics Research Program was established in the fall of 2009 by Brown University to facilitate advances in translational research concerned with the genetics of developmental disorders. |
Contact: Rebecca Kartzinel
Location: Biomed Center »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Herbarium | Dried and pressed plant specimens mounted on sheets of archival paper. Plant data. | The collection includes around 100,000 plant specimens and is an important depository of Rhode Island and New England collections. It is also rich in western and southern North American plants and includes special sets of historically valuable specimens from 19th and early 20th century western US expeditions. Among other important collections, the herbarium also includes a full set of Charles Wright's Cuban plants (1856-1867) and a unique and classic collection of Carex |
Contact: Roxanne Wood
Location: 233 Richmond Street, Providence, RI 02903 »See on the MapAffiliated Centers: Brown University Oncology Research Group (BrUOG)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Oncology Contact: Roxanne Wood | Clinical Trials Support, Oncology Research | The Brown University Oncology Research Group (BrUOG) mission is to improve cancer care through the implementation of innovative, multidisciplinary cancer clinical trials. BrUOG provides the infrastructure for the efficient development and implementation of these trials, which are created by Brown University faculty. BrUOG’s administrators and physicians provide support for the initial study concept and validation of trial design, and are responsible for trial administration, safety monitoring, data analysis, and the presentation and publication of findings. The trials sponsored by BrUOG investigate novel, cutting-edge applications of chemotherapy, biologic agents and other cancer treatments. Clinical trials are available for a broad range of disorders in hematology/oncology and these include treatment for cancers of the breast, brain, lung, gastrointestinal tract, skin, and prostate as well as trials in leukemia and lymphoma. |
Contact: Norka De La Rosa Ramirez
Affiliated Centers: Brown-Lifespan Center for Digital Health
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Emergency Digital Health Resources | Clinical Research Resources, Emergency Digital Health | EDHI’s mission is to use digital health to transform the care of patients with acute care needs before, during, and after their visit by developing and deploying effective, evidence-based digital health tools. The goal is to use technology to improve access to patients and increase the efficiency of health care delivery. As this field rapidly expands, it is critical for us to develop theoretically based interventions and to provide evidence that digital health tools are acceptable and effective, for both clinicians and patients. EDHI’s vision is for digital health to be an evidence-based, effective and well-integrated tool for creating and restoring health. |
Contact: Carney Institute
Location: 164 Angell St., 4th Floor Providence, RI 02912 »See on the MapAffiliated Centers: Carney Institute for Brain Science
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Brain Science | Clinical Research Resources, Brain Science | The Carney Institute for Brain Science (formerly the Brown Institute for Brain Science –BIBS) advances multidisciplinary research, technology development, and training in the brain sciences and works to establish Brown University as an internationally recognized leader in brain research. The Institute unites more than 100 faculty from a diverse group of departments at Brown, spanning basic and clinical departments, and physical and biological sciences. The Carney Institute provides a mechanism to advance interdisciplinary research efforts among this broad group and provides essential support to obtain and administer multi-investigator grants for research, infrastructure, and training. The Institute actively seeks new training funds to support interdisciplinary education that transcends that available in individual academic departments. |
Contact: Lauri Bazelman
Location: The Miriam Hospital, CFAR Building, Room 134 »See on the MapAffiliated Centers: Center for AIDS Research (CFAR) - Providence/Boston
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - BioBehavioral Research Support, HIV Contact: Curt Beckwith BioBehavioral Sciences Webpage | Clinical Research Service, HIV Research | The BioBehavioral Sciences Core (BBSC) provides help with participant recruitment, enrollment, and retention to advance biobehavioral research; services to support innovative medical and behavioral data collection approaches, including methods, tools, and assessments; expertise and services related to regulatory and human subjects’ compliance, and the ethical conduct of research for vulnerable populations; provide expertise and services to support the development, implementation, and evaluation of interventions to improve HIV prevention and continuum of care outcomes; specialized services for the conduct of implementation science research and for performing cost-effectiveness research; PrEP related services and information; and guidance and support for accessing EMR data. |
Research - Biostatistics, HIV Contact: Joseph Hogan, ScD Biostatistics Webpage | Clinical Research Service, HIV Research | The Biostatistics Core ensures that all research undertaken by Center for AIDS Research (CFAR) investigators uses optimal methods for study design and analysis. The Core provides guidance and collaboration in developing grant applications; collaboration on study design, conduct, and statistical analyses; statistical training and mentoring; facilitation of linkages to potential collaborators; provides guidance and support for accessing EMR data; facilitates access to HIV databases from other centers/programs, and develops novel statistical and epidemiological methods for HIV research. |
Research - Substance Use Research Core, HIV Contact: Christopher Kahler, PhD Substance Use Research Core Webpage | Clinical Research Service, HIV Research | The Substance Use Research Core (SURC) offers services and expertise on the methods needed to conduct effective Substance Use (SU)/HIV research. The SURC offers expertise in innovative methods for assessing and analyzing SU, and developing and implementing interventions to address alcohol, tobacco, and other drug use. Services include: expertise in developing research projects that address gaps in SU/HIV priority issues both domestically and globally; advice on training on the most appropriate methods for assessing SU; advice and training on incorporating effective and up-to-date SU interventions; and guidance on addressing ethical issues with SU research including input on IRB protocols and human subject sections of grant applications and access to group trainings. |
Administration - CFAr Contact: Vicki C. Godleski Administrative Core Webpage | Clinical Research Service, CFAR Coordinating Center | The CFAR Administrative Core has built an HIV infrastructure across the partner institutions and provides oversight of the entire CFAR community with responsibility for sound management, organizational structure, appropriate resource allocation, evaluation and the promotion of inter/multi-disciplinary translational HIV research. Through efficient communication to CFAR membership, the Core manages a highly interactive and collaborative scientific environment across geographically dispersed settings in domestic, national and international sites. |
Research - Basic Sciences, HIV research Contact: Rami Kantor, MD Basic and Translational Sciences Webpage | Clinical Research Service, HIV Research | The Basic Sciences Core builds and supports collaborative research and synergy between basic, translational clinical and behavioral investigators as well as other multidisciplinary scientists, by providing laboratory, consultation and mentoring services. Services include; Viral load measurement, STI testing, Genital tract specific assays, Biologic specimens collection techniques, HIV-1 and 2 drug resistance assays, Identification of HIV dependency factors (HDF), Cell culture, Viral stocks, Immune activation, Laboratory Consulting, Training and Mentoring. Additional assays may be developed as necessary, please contact the Core Director. |
Contact: Fatemeh Akhlaghi
Location: Laboratory 490, Avedisian Hall »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Pharmacokinetics Consultation Contact: Fatemeh Akhlaghi | Clinical Research Resources, Pharmakokinetics | The laboratory is capable of providing support for the design and implementation of pre-clinical and clinical pharmacokinetics studies as well as design and conduct of ancillary in vitro studies (protein binding and microsomal incubations to characterize phase 1 or 2 metabolism). With extensive background in pharmacokinetics modeling and simulation using NONMEM, WinNonlin and Simcyp softwares, the laboratory can provide a valuable resource for on-going or completed clinical studies. Additional data analysis capabilities includes in-vitro in-vivo correlation (IVIVC) and bioequivalence analysis. |
Pharmacokinetics, Bioanalytical support Contact: Fatemeh Akhlaghi | Clinical Research Resources, Pharmakokinetics | The Clinical Pharmacokinetics Research Laboratory is equipped with two UPLC-MS/MS and one quadrupole-time-of-flight mass spectrometer (ESI-QTOFMS) systems capable of measuring the concentration of small molecule xenobiotics and their metabolites as well as endogenous or therapeutic peptides and proteins. To date, this laboratory has developed various assays for the quantification of up to 60 different compounds and validated these assays according to guidelines by the Food and Drug Administration of the United States. The laboratory is proficient in providing analytical support for on-going pre-clinical and clinical studies and during different stages of a drug development project. |
Contact: Gaurav Choudhary
Location: The Providence VA Medical Center »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Clinical Research and Development Service Resources | Clinical Research Resources, Veteran Population | The VA Research's fundamental mission is to advance the healthcare of Veterans. The Providence VA Medical Center has a large funded research and development program, including studies in Rehabilitation Medicine, Cardiology, Mental Health, Neuroscience, Substance Abuse, Health Services & Economics, and Pulmonary Disease. These studies enhance our ability to provide state-of-the-art techniques and treatments to our patients. At the same time, the research program helps Providence VAMC recruit and retain the highest quality health care professionals who treat our Veterans in the VA Clinics on a daily basis. |
Contact: Pam Borek
Affiliated Centers: IDeA COBRE for Stress, Trauma and Resilience (STAR) at Miriam Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Contact: Ashok Ragavendran
Affiliated Centers: Center for Computation and Visualization (CCV), IDeA COBRE Center for Computational Biology of Human Disease at Brown University
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analysis - Data Analysis Contact: CBC help | Service | The core provides assistance in experimental design and data processing pipelines for high-throughput datasets generated in individual projects, particularly for DNA/RNA sequencing data. |
Analysis - Standardized Data | Service | Develop/Implement Bioinformatic Pipelines; GATK whole exome, Cut and run. Develop/Implement Standardized QC for Workflows. Experimental Design Support; Power Analysis for Microbiome and RNAseq. Exploratory Data Analysis; RNAseq, scRNA seq. |
Tooling qckitfastq | Software - R-Qckit | A comprehensive quality control R package for Next Generation Sequencing FASTQ data. |
Tooling RSeqAn | Software - RSeqAn | Wrappers for the SeqAn library for use in R with Rcpp |
Tooling bioflows | Software - Bioflows | A bioinformatics workflow engine that streamlines the most common analysis pipelines. Key features include: 1) Provision of standardized workflows 2) A module for automating SRA downloads and 3) External tools are made available as CONDA packages along with the tool. |
Tooling | Software - Scripts | Access Genbank, BioCookBook.jl |
Tooling NMRdock | Containerization with Docker - NMRDock | NMRdock is a Docker container image for processing raw NMR time domain data into frequency domain spectra and analyzing the spectra. The image includes NMRPipe and NMRFAM-Sparky for processing and analysis, respectively. NMRdock can be installed on any operating system (OSX, Windows, or Linux) and has the full functionality of both NMRPipe and NMRFAM-Sparky. For guides on processing BioNMR data in NMRdock, please access our Walkthrough Section |
Tooling | Containerization with Docker | IDeP |
Tooling Ingenuity Pathway Analysis Request a Service | Software- Ingenuity Pathway Analysis | The CBC provides access to the Ingenuity Pathway Analysis web server at Brown University. |
Analysis - Data Analysis | Service | The CBC now allows for a very restricted schedule of Fee-For-Service project. |
Data Management RefChef | CCV - RefChef | RefChef is a reference management tool used to: (1) document the exact steps undertaken in the retrieval of genomic references; (2) maintain the associated metadata; (3) provide a mechanism for automatically reproducing retrieval and creation of an exact copy of genomic references. |
Training and Support Workshops | Service | Workshops |
Training and Support Request a Service | Service - Consulting | The Core provides two distinct complimentary services to all researchers at Brown. Appointment Based, A team of experienced computational biologists and biostatisticians are on-hand to help investigators design experiments. Weekly Consulting Sessions, The Core offers two hours *walk-in Consulting* sessions for all researchers to discuss any immediate issues in data analysis including trouble-shooting code, help with statistical analysis and discussion of options for bioinformatic analysis. |
Training and Support | Service -Talent Development | Student worker/interns, Guidance and mentoring |
Training and Support Outreach | Service - Outreach | CBC hosts workshops, publishes tutorials on computing tools used by bioinformaticians, and provides analysis support to the Brown community. |
Contact: Patrycja Dubielecka-Szczerba, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Advise investigators with experimental design, implementation and data analysis. Provide support for grant submissions. |
Contact: carney-institute@brown.edu
Affiliated Centers: Carney Institute for Brain Science, IDeA COBRE Center for Central Nervous System Function at Brown University
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Design - Experimental Design | Clinical Research Resources, Behavior and Neuroimaging | |
Multilevel and Hierarchical Modeling | Clinical Research Resources, Behavior and Neuroimaging | |
Machine Learning | Clinical Research Resources, Behavior and Neuroimaging | |
Feature Extraction | Clinical Research Resources, Behavior and Neuroimaging | |
Effective and Functional Connectivity | Clinical Research Resources, Behavior and Neuroimaging | |
Spatial-Temporal Modeling | Clinical Research Resources, Behavior and Neuroimaging | |
Time Series Modeling | Clinical Research Resources, Behavior and Neuroimaging | |
General Linear Model (GLM) and Linear Model (LM) | Clinical Research Resources, Behavior and Neuroimaging | |
Analysis of Variance (ANOVA) | Clinical Research Resources, Behavior and Neuroimaging | |
Regression for High Dimensional Data | Clinical Research Resources, Behavior and Neuroimaging | |
Analysis - Network Analysis | Clinical Research Resources, Behavior and Neuroimaging | |
Causal Interference | Clinical Research Resources, Behavior and Neuroimaging |
Contact: Kristin Fortin-Ashburne
Location: Butler Hospital »See on the MapAffiliated Centers: IDeA COBRE Center for Neuromodulation (CCN) at Butler Hospital, Carney Institute for Brain Science
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Design and Analysis Services Contact: Kristin Fortin-Ashburne Quantitative Sciences Program | The Design and Analysis Core (DAC) provides experimental design, statistical support, and neuroimaging consultation services to COBRE Project Leaders and the broader scientific research community. | DAC faculty are affiliated with the Quantitative Sciences Program (QSP) of the Departments of Psychiatry and Neurology at the Alpert Medical School of Brown University and the Center for Neurorestoration and Neurotechnology at Providence VA Medical Center. Core Leader Richard Jones, ScD is a psychiatric epidemiologist and methodologies, and the director of the QSP. Core Co-Leader Jennifer Barredo, PhD is a neuroscientist with expertise in neuroimaging methodologies including advanced diffusion imaging, multimodal analysis, and machine learning. The DAC also maintains scientific computing resources and workflows available to COBRE project and pilot investigators and sponsors educational seminars and workshops. |
Contact: Chuck Toth
Location: BioMedical Center Building »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Drosophila Media Preparation | Service | The Drosophila Media Facility is dedicated to providing investigators with high quality media for Drosophila research. This centralized kitchen is equipped with two food-service quality Cleveland Range Kettles (MKET-12-T). Media is pumped into trays of vials or plastic bottles using a custom built fly food dispenser. Researchers using glass vials return them to the facility where they are autoclaved, washed, and filled again. This centralized kitchen is equipped with a food-service quality Cleveland Range Kettle MKET-12-T. Media is pumped into trays of vials or plastic bottles using a Gilson Fly Food Dispenser. Used glass vials are returned to the facility where they are sterilized in a Steris autoclave and washed in a Northwestern Systems G403 electric heat glassware washer. |
Contact: Shengliang Zhang, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Advise interested investigators on cancer drug screens, cell-based assay development, assay optimization, and use of various chemical libraries/FDA-approved drugs to support research in the area of drug discovery and development. Advise on target validation, and interface with medicinal chemistry. Provide technical support for screening implementation, library plating, high-throughput robotic screening, and advice on prioritization of hits. Provide support for grant submissions. |
Contact: Joseph S Boesenberg
Location: Geo-Chem Building »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analyzer - Electron Microprobe Contact: Stephen Parman | Cameca SX100 Electron Microprobe Analyzer | The Cameca SX-100 electron microprobe is equipped with 5 wavelength dispersive spectrometers (WDS) and a Rontec energy dispersive spectrometer (EDS).The WDS spectrometers each contain two or four diffracting crystals and presently include LTAP, TAP (2), LPET (2), PET, LLIF (2), LIF, LPCO, PC1 and PC2. The last three crystals are used to measure the light elements (boron to sodium). The absolute detection limits for the instrument are approximately 50-200 ppm, depending on the element. The EDS spectrometer is capable of detecting all elements above sodium (Na). X-ray element maps can easily be acquired on the microprobe. Using 512x512 pixel maps, up to nine element maps (5 elements on WDS, 4 with EDS), plus a backscattered electron (BSE) image, can be obtained simultaneously. Maximum resolution on any one map is one micron per pixel. Utilizing the coordinate system, maps of entire thin sections can be generated. |
Contact: Anthony McCormick
Location: Room 020D, Engineering Research Center Building »See on the MapAffiliated Centers: Instrumentation for Molecular and Nanoscale Innovation (IMNI)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Microscope - Focused Ion Beam Electron (FIB) | Thermo/FEI Helios Dual Beam Focused Ion Beam Electron Microscope (FIB) with an Omniprobe nanomanipulator and autoTEM sample preparation | The FIB is fully equipped for (near) simultaneous ion/electron beam imaging and patterning of specimens. A Pt metal deposition source is available for ion (or electron) beam assisted deposition of conducting lines. A second source is available to enhance the carbon removal rate. An Omniprobe AutoProbe 200.2 allows the physical manipulation of samples with nanometer precision and is used along with Pt metal deposition and standard FIB trenching to allow the selection and removal of electron transparent TEM samples from specific microstructural features. A NPGS pattern generation system (plus beam blanker and external scan interface) controls the electron beam for high resolution, interlaced pattern generation used in the FIB fabrication of nanostructures. Three dimensional crystallographic data can be generated from specimens using the fully automated EBSD system (Oxford HKL&EBS-3). The microscope is also equipped with an EDS detector for elemental analysis. An electron flood gun can be used with the ion beam to image/etch nonconductive samples. A low temperature holder is available for beam sensitive samples. |
Microscope - Scanning Electron (SEM) | LEO 1530 High Resolution Scanning Electron Microscope (HRSEM) | The LEO 1530 VP ultra-high resolution field emitter SEM that allows for a resolution of 1 nm at a voltage of 20 kV in high vacuum and 2 nm at 30 kV in the variable pressure mode. It is fully equipped with EDS for chemical mapping and includes a Debbem tensile test stage 5kn. The instrument is equipped with Nabity pattern generating software a high speed beam blanker and is set-up for high resolution electron-beam lithography. The LEO 1530 VP is capable of a resolution of 2.1 nm at very low electron energies (1 kV) which is of great use for beam-sensitive materials like polymers and insulating surfaces. Also very useful for insulating samples is the variable operating pressure mode which allows insulating materials to be imaged without coating the surface. |
Microscope - Transmission Electron (TEM) | FEI CM20 TEM | The FEI CM20 is available for applications that require high tilt, moderate resolution (0.23 nm). This 200 kV analytical TEM allows the large specimen tilts needed for dislocation characterization and weak-beam imaging applications. It is equipped with a low background double tilt holder and a liquid nitrogen temperature holder. Compositional analysis is performed on this instrument using energy dispersive x-ray spectroscopy. Images are recorded using a large field of view, side mounted, digital image acquisition system. |
Microscope - Scanning Transmission Electron Microscope -S(TEM) | JEOL 2100F - S (TEM) | The 2100F is a 200 kV, field emission source, high-resolution/analytical S(TEM) capable of 0.10 nm lattice resolution. It is primarily used for obtaining lattice images or when chemical or crystallographic information is required from laterally small areas (1-10 nm). The instrument is equipped with an x-ray spectrometer capable of light element detection, a Gatan image filter (GIF) for element and phase analyses and enegy filtered imaging, a high tilt holder for electron tomography and a high-resolution CCD camera system for digital image acquisition and processing. A low background double-tilt holder for chemical analysis by EDS, a standard double tilt holder, a heating holder capable of 1000C, and a heating straining holder are available. Probes as small as 1 nm in diameter are accessible for micro-diffraction experiments. STEM mode for EDS line and area scans, bright and dark field STEM detectors, and high angle annular dark field is capable in STEM mode. |
Spectrometer - ThermoElectron K-Alpha XPS | Thermo/FEI K-Alpha X-Ray Photoelectron Spectrometer | This equipment is used to study the surface composition of materials using photoelectrons generated by 30 kV monochromated Aluminum K-Alpha x-rays hitting the sample. It is equipped with an Argon sputter gun for surface cleaning and depth profiling. There is a tilt and rotate holder. There is a transfer holder so samples can be transferred to the XPS with exposure to atmosphere. |
Microscope - Quattro S-E(SEM) | Thermo/FEI Quattro S-E(SEM) | The Quattro is an environmental SEM which means that the sample chamber can be set to a (relatively) high pressure for water containing samples. This SEM has EDS (X-ray detection) and EBSD (electron backscatter). The Quattro S is a field emission scanning electron microscope capable of generating and collecting all available information from any type of sample material. It provides the most versatile high-resolution low-vacuum FEG SEM with extended low-vacuum capabilities and the most versatile high resolution SEM for characterization, prototyping and in situ analysis. The features include three vacuum modes enabling investigation of conductive, non-conductive and high-vacuum incompatible materials such as High-vacuum mode (<6*10-4 Pa) for imaging and microanalysis of conductive and/or conventionally prepared specimens; Low-vacuum mode (10 to 130 Pa) for imaging and microanalysis of non-conductive specimens without preparation; and ESEM™ mode (10 to 4000 Pa) for high-vacuum incompatible specimens which are impossible to investigate with traditional EM methods. The system is equipped with a 5-axes motorized x-y-z-tilt-rotate stage,for Sample navigation. |
Sample Preparation | Electron Microscope Facility Sample Preperation Area | Tools available in the EMF sample preparation area include a jet polisher, polishing benches, dimpler and high and low speed diamond saws.The sample prep area has a carbon thread evaporator and Gold/Palladium sputter coater for making conductive coatings on SEM samples. There is an ion mill (PIPS) for making TEM samples. There is also a plasma cleaner for TEM sample holders and SEM samples. |
Contact: Gregory Jay
Location: Coro WestInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Emergency Medicine Research Laboratory Resources Contact: Francesca Beaudoin | Clinical Trials Support, Emergency Medicine | The research program in the Department of Emergency was established to support investigator-initiated research and pharmaceutical, biologic and biomedical device trials. The program is supported by a Research Program Administrator, two pre-award Research Administrators, a Clinical Research Supervisor, and a staff of Clinical Research Assistants. The department maintains clinical research space within emergency departments as well as fully-equipped basic science laboratories, and has ongoing collaborations with Brown University and other hospital departments.The main focus areas of the Department of Emergency Medicine research program are (1) injury prevention and control, (2) cardiovascular, neurologic, and resuscitation science, (3) pediatric, age, and gender specific care, and (4) healthcare systems science. |
Contact: Marcelo Alexandre
Location: MacMillan Hall »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analyzer - Elemental Analyzer | Thermo Scientific, FlashSmart EA | Analysis of carbon, nitrogen, and sulfur concentration of solid samples |
Analyzer - Inorganic Carbon Analyzer | UIC, INC Inorganic Carbon Analyzer | Measures total inorganic carbon by automated acidification and coulometric detection in a wide variety of sample matrices. Solid samples ranging from low ppm to 100% inorganic carbon can be analyzed with this system. |
Sample Preparation - Freeze Dry System | Labconco FreeZone 6 Liter | For removing water and other solvents from frozen samples by sublimation. |
Liquid Chromatography System | Dionex DX500 | Ion chromatography analysis of anions and cations in marine and freshwater samples |
Sample Preparation - Microwave Digestion System | Milestone Ultrawave | Used to digest solid samples (soils, sediments, rocks, plants, tissues), for elemental analysis. |
Spectrometer - Atomic Absorption Spectrometer-Graphite Furnace (GFAAS) | Perkin Elmer AAnalyst 600 Graphite Furnace | This spectrometer is useful for measuring trace levels of metals in water and soil. |
Spectrometer - Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) | Thermo Scientific iCAP 7400 DUO ICP-OES | Analysis of most elements with atomic weights above 10. |
X-Ray Fluorescence - Handheld | Innov-X Systems Alpha 4000 Handheld | Non-destructive elemental analysis on a variety sample types for concentrations of some metals ranging from about 20 ppm to 10%. |
X-Ray Fluorescence - Handheld | Olympus Delta Professional Handheld | Non-destructive elemental analysis on a variety sample types for concentrations of elements greater than 10 ppm. |
Mass Spectrometer - Isotope Ratio Mass Spectrometer | Thermo 253+IRMS | Dedicated to determine the triple oxygen isotopic composition (17O/16O and 18O/16O) of silicates, carbonates, gas and waters |
Liquid Chromatography - Mass Spectrometer | Agilent 1200 LC/MS System | Used to separate and then quantify the chemical components in a given sample. |
Gas Chromatography - Flame Ionization Detector | Agilent 7090B GC-FID | Used to measure the trace levels of semivolatile organic compounds in a given organic extracted sample. |
Gas Chromatography - Mass Spectrometer | Agilent 5977B GC-MSD | Used to determine molecular composition and the concentration of organic compounds in organic extracted samples |
Mass Spectrometer - Isotope Ratio Mass Spectrometer | Thermo Scientific GC-C-IRMS Delta V Plus | Used primarily for compound specific ð13C in organic extracts |
Accelerated Solvent Extractor (ASE) | ASE 200 Dionex | Used to extract organic compounds from solid samples. |
Contact: Sicheng Wen MD, PhD, Director
Location: CORO West Research Building »See on the MapAffiliated Centers: IDeA COBRE Center for Stem Cells and Aging at Rhode Island Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Centrifuge - Ultra | Thermo Fisher WX80 ultracentrifuge | Ultracentrifugation is the classical method for extracellular vesicles isolation. The Extracellular Vesicle Core contains two Thermo Fisher WX80 ultracentrifuges with Sorvall AH-629 rotors. |
PCR Thermal Cycler | ViiA 7 real-time PCR system with twister robot | We identify extracellular vesicles MicroRNA/mRNA profiling using the ABI TaqMan PCR array by ViiA 7 Real-Time PCR System, which is fully compatible with any standard or fast-cycling 384- or 96-well plates and TaqMan Array Micro Fluidic Cards and allows quickly and efficiently look up to 348 unique microRNAs/mRNAs per sample with as little as 1ng of input total RNA with pre-amplification. |
Analyzer - Nanoparticle Counter | NanoSight 5000 Nanoparticle Counter | Nanoparticle tracking analysis (NTA) – direct and real-time visualization and analysis of nanoparticles in liquids, and calculate the size, size distribution and concentration of the particles. Brownian motion of nanoparticles is analyzed using the Stokes-Einstein equation. |
Ultrafiltration | TangenX | By using tangential flow filtration (TFF) technology, TangenX delivers rapid and highly efficient isolation of extracellular vesicles from large volumes of samples. |
Contact: Kevin Carlson
Location: Room 283, BioMedical Center Building »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Fluorescence Activated Cell Sorter (FACS) | Becton Dickinson (BD) Biosciences FACS Aria III | Cell counting, sorting, biomarker detection. The FACSAria has five air-cooled lasers at 351nm, 488nm, 561nm, 633nm, and 407nm allowing multicolor analysis of up to 20 parameters. It can sort 1 to 4 populations simultaneously and is equipped with an Automated Cell Deposition Unit, an Aerosol Management System and a refrigeration system. It can accept various sample input tubes including 1 mL microtubes, 12 x 75-mm test tubes, and 15 mL tubes into the sample and collection chambers. |
Flow Cytometer - Benchtop Analyzer Cytek Info | Cytek Aurora | The Cytek Aurora can analyze similar and very similar spectral emitting fluorochromes. Instead of analyzing small chunks of fluorescence and calculating fluorescence spill over, the Cytek Aurora looks at the full spectrum emitence of each fluorochrome. This allows colors that would normally be unusable with each other to be run in tandem. The Cytek Aurora can analyze up to 54 fluorochromes, 41 parameters (FSC, 488 SSC, 405 SSC). |
Fluorescence Acticated Cell Sorter (FACS) | Becton Dickinson (BD) Biosciences FACS Celesta | Cell counting, biomarker detection. The FACSCelesta has three air-cooled lasers at 488nm, 561nm and 407nm allowing multicolor analysis of up to 15 parameters that has an attached high throughput system (HTS) that allows users to analyze samples directly from a 96 well plate. |
Contact: Mark Dooner
Location: Coro West Building, Suite 5.08A, »See on the MapAffiliated Centers: IDeA COBRE Center for Stem Cells and Aging at Rhode Island Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Fluorescence Activated Cell Sorter (FACS) | Becton Dickinson (BD) Biosciences LSRII Analyzer/Flow Cytometer | Digital, 4 laser (355nm, 405nm, 488nm and 635nm) flow cytometric analysis system with 10 fluorescent channels plus 2 scatter channels. BD FACSDiva software with digital compensation and ModFit software for cell cycle analysis. |
Mass Cytometry Legorreta Cancer Center | Fluidigm Helios (CyTOF) Mass Cytometry | Mass cytometry combines the technologies of flow cytometry with that of mass spectrometry. It applies nebulization, aerosolization and ionization to cells labeled with heavy metal isotope-conjugates, allowing for ion separation based on their mass:charge ratio and quantification of each isotope on a per cell basis ultimately determining properties of a cell population at a single-cell resolution. The technique of mass cytometry is powerful, as it can analyze more cellular targets without the problem of compensation as seen in flow cytometry. The Helios is capable of simultaneously analyzing up to more than 50 parameters both on cell surface and intracellularly. |
Fluorescence Activated Cell Sorter (FACS) | Becton Dickinson (BD) Biosciences Influx Cell Sorter | 5 laser system (355nm, 405nm, 488nm, 561nm and 638nm) for simultaneous detection of up to 14 parameters. Sorts up to 4 different cell populations. Capable of single cell deposition into many different sized culture plates (96 or 384 well plates, etc). Equipped with a small particle objective for FSC size determination down to 0.5 micrometers. Enclosed in a biosafety hood. The sorter separates particles on the detection of fluorescent markers on particles such as cells, chromosomes, bacteria or nuclei. Then the collected cells are suitable for downstream analysis either in molecular applications like protein assays and PCR, or used in viable assays such as tissue culture or injected into in vivo models. Generated data may also be used to determine percentages of interrogated populations. The sorter can separate populations into tubes (1.5ml, 4ml, 15ml, 50ml), multi-well plates (6, 24, 48, 96, 384), or directly onto microscope slides. |
Training and Technical Assistance | Service |
Contact: Kristine Pelton
Location: BioMed Center, 85 Brown St, RM106 »See on the MapAffiliated Centers: Center for Alzheimer's Disease Research
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analyzer - HD-X™ Automated Immunoassay | Quanterix | The SIMOA® HD-X™ Analyzer is an ultrasensitive protein biomarker analysis platform that uses single-molecule array (Simoa®) technology, which enables sensitive and specific measurement of low-abundance proteins in complex biological samples such as serum, plasma, CSF, urine, saliva, as well as cell and tissue lysates. The HD-X™ system features high-throughput processing and enables simultaneous use of digital (single molecule counting) and analog (integrated fluorescence intensity) measurements to provide ultra-sensitive single molecule detection with dynamic range of 4-5 logs. |
Analyzer - Meso Scale QuickPlex® SQ 120MM | Meso Scale | Meso Scale Discovery (MSD) QuickPlex® SQ 120MM is a high-throughput multiplex assay platform for protein and biomarker analysis. The QuickPlex 120 system is capable of measuring up to 10 different protein analytes in a single well of a 96-well plate, making it ideal for large-scale and complex studies. It uses electrochemiluminescence technology to deliver highly sensitive and reproducible results. Additionally, MSD offers a range of pre-validated assays for a variety of applications including neurodegeneration, neuroinflammation, intracellular signaling, toxicology and phosphorylation analysis. The QuickPlex 120 system is designed to support basic research, drug discovery, and clinical diagnostics with the option for easy-to-build custom multiplex assays. |
Analyzer - SMCxPRO | Sigma | The SMCxPro® analyzer uses both plate-based and bead-based singleplex assay designs for the quantification of protein-based targets in highly complex samples including serum, plasma, CSF and saliva. Additionally, the digital quantification of individual molecules provides fg/ml sensitivity required for detection of blood based protein biomarkers. The SMC™ technology allows the measurement of proteins with increased precision, enabling quantification at both low and high expression levels. |
Contact: Christoph Schorl, PhD
Location: Room 109, Laboratories for Molecular Medicine »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Bioanalyzer | Agilent Bioanalyzer 2100 | Microfluidics-based platform for sizing, quantification and quality control of DNA, RNA. |
Analyzer - Fragment | Advanced Analytical, Fragment Analyzer | Capillary Electrophoresis - Analyze DNA fragments for Next-Gen Sequencing, genotyping, checking RNA quality and quantity, and working with microsatellite simple sequence repeats (SSRs). |
Cell Counter | Life Technologies Automated Countess Cell Counter | Measure cell count and viability (live, dead, and total cells) using the standard trypan blue technique. |
Centrifuge | Sorvall Legend T Benchtop | Low to medium speed centrifugation |
Centrifuge - Ultra | Beckman Coulter Optima Max UltraCentrifuge | High speed centrifugation with MLA130, TLA110 & TLA100 Rotors |
Disrupter | Covaris S220 High Performance Ultrasonicator | DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization |
Imaging System - Gel Imager | LI-COR Odyssey Clx IR (Infrared) Imaging System | Western blot analysis, EMSA, protein arrays, In-Cell Western Assays, On-Cell Westerns, in vivo imaging, Coomassie gel documentation, DNA gel documentation, and tissue section analysis. |
Nanostring GeoMX DSP Contact: Christoph Schorl | Spatial analysis of gene or protein expression. | |
PCR Thermal Cycler | Applied Biosystems ViiA 7 Real-Time PCR System | Medium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput |
PCR Thermal Cycler | Applied Biosystems ViiA 7 Real-Time PCR System | Medium to high throughput 96- and 384-well fast real time quantitative PCR formats, medium to high throughput |
Spectrophotometer | Thermo Scientific Nanodrop One | A microvolume spectrophotometer for measuring DNA and RNA. |
Technical Assistance - Affmetrix Microarray | Service | Service to provide Affymetrix gene expression array service |
Technical Assistance - NGS Library Quality Control | Service | Analysis of library size distribution and qPCR quantification |
Imaging System - Odyssey | Li-Cor | |
Analyzer - nCounter Systems | NanoString - nCounter Sprint Profiler | Molecular barcoding technology for RNA, DNA, and protein profiling applications. Digitally examine multiple pathways in a single tube in an extraction-free workflow. Included nSolver Analysis Software. Compatible with FFPE tissue. Highly multiplexed analysis of many functional pathways such as among others cancer, inflammation, cell death and custom designed expression analysis. Operated as fee for service. |
Imager and Microplate Reader | BioTek Cytation 5 Cell Imaging Multi-Mode Reader | UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence. Cell migration and invasion assays, Live cell imaging, Fluorescence cell imaging (DAPI & GFP), Multi-mode reader applications, Immunofluorescence, Phenotypic assays, Histology |
PCR Thermal Cycler - Droplet Digital | Bio-Rad QX200 Droplet Digital (ddPCR) PCR System | Provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications. |
DNA Sequencer | Illumina - NextSeq550 | The NextSeq550 Next Generation Sequencing system is a high throughput bench top sequencer that provides accurate fast sequencing data, and by employing flexible run configurations it can be easily adopted to a researcher’s needs depending on their required read length and sequencing depth. Reagents are available in a mid-output and a high output mode resulting in maximum cluster numbers of 400 million (high output) and 130 million (mid output) clusters (reads). |
Microplate Reader | BioTek Synergy H1 Hybrid Multimode (H1MM) Microplate Reader | UV-Vis absorbance, Fluorescence intensity, Luminescence, Fluorescence polarization, Time-resolved fluorescence. Cell based assays, Biomarker assays, Micro-volume analysis of nucleic acid and protein, Kinetic assays. |
Contact: John McGeary, PhD
Location: Research Building 32, Room 146 »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Autoclave | Tuttnauer | Equipment: consumable sterilization |
Bioanalyzer | Agilent Bioanalyzer | DNA size & quantification (quality check for downstream analyses, i.e. next gen sequencing) |
Analyzer - Genetic | Applied Biosystems 3500 Genetic Analyzer | Capillary Electrophoresis - Analyze fragment length polymorphisms (96 format) (2) |
Centrifuge | Eppendorf Refrigerated Centrifuge | Alcohol precipitation |
Centrifuge | Eppendorf | For centrifuging 96/384 plates |
DNA Sequencer | Ion Gene Studio/S5 with Ion Chef/ThermoFisher | Next Generation Sequencer |
Mass Spectrometer | Sequenom | high throughput SNP genotyping, epigenetics |
Microarray Scanner | Illumina iScan | Whole-genome genotyping, gene expression analysis, methylation analysis |
Microplate Reader | BioTek | DNA quantification |
Microplate Reader | Fluidigm End Point Reader | High throughput SNP genotyping |
PCR Thermal Cycler | Applied Biosystems | 384 well DNA amplification for downstream analysis (i.e. sequenom processes) (2 units) |
PCR Thermal Cycler | Applied Biosystems | 96 well DNA amplification for downstream analysis (i.e. fragment length polymorphisms)(2 units) |
Robotic Workstation - DNA Extraction | Chemagen/Perkin Elmer Magnetic Separation Module | DNA extraction from blood & saliva |
Robotic Workstation - Liquid Handler | Sequenom Liquid Handler | Transfers PCR chemistries to samples (96/384 format) |
Robotic Workstation - Liquid Handler | Sequenom Nanodispenser | Dispenses PCR product to Sequenom chip |
Robotic Workstation - Sample Preparation | Iontorrent/Life Technologies Enrichment System | Simplifies the workflows for the Ion PGM system by providing an automated solution for scalable and reproducible template preparation. |
Robotic Workstation - Sample Processing | Thermo Scientific Cap-it-All | Caps tubes to the optimal torque to maintain sample integrity and provide a hands free method to capping and decapping tubes quickly and easily. |
Robotic Workstation -Liquid Handler | Beckman Coulter Lab Automation Workstation | Automated liquid handler |
Spectrophotometer | Thermo Scientific NanoDrop | DNA quantification |
DNA Sequencer Contact: Kayla Dwyer | Qiagen Pyromark Q96 ID | Quantitative analysis of genetic/epigenetic DNA modifications using Pyrosequencing technology |
PCR Thermal Cycler | Applied Biosystems Real Time PCR Quant Studio 12K Flex | Analyze single nucleotide polymorphisms (single locus) (96/384 format) |
Robotic Workstation-Sample Preparation | Tecan Freedom Evo | Simplifies the workflows for the Illumina HiScan system by providing an automated solution for scalable and reproducible sample preparation. |
Contact: John Cronan, MD
Location: Main Building, 3rd Floor »See on the MapAffiliated Centers: Carney Institute for Brain Science, Norman Prince Neurosciences Institute
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analysis - Image Analysis and Education | Advanced computing for image analysis, an image processing server, conference room and three offices. | The Hospital-Imaging Research and Education Service (HI-RES) Facility has two image analysis and education centers, one at Rhode Island Hospital and the other at Butler Hospital. Research mission: To foster collaborative imaging research both in/within stakeholder departments and also to foster collaborative links to Brown University campus-based researchers. This has the potential to include trainees interested in hands-on research projects as well as hospital-based faculty. Education mission: To bolster the interdisciplinary education of hospital-based trainees in the use of imaging, particularly neuroimaging, to advance the understanding of the biological basis of neurodevelopmental disorders. This will include didactic sessions, journal clubs and hands-on sessions with trainees, including medical residents and PhD- trainees. |
Contact: Jennifer Tidey, Ph.D.
Location: Brown University School of Public Health »See on the MapAffiliated Centers: IDeA COBRE Center for Addiction & Disease Risk Exacerbation (CADRE) at Brown University
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analyzer - Automated Immunoassay System Contact: CADRE | ProteinSimple - Ella Next Generation ELISA system | The Ella mutiplex analyzer allows for multi-plexing of multiple biomarkers on the same cartridge. Rather than performing a separate ELISA for each biomarker with more sample volume to run in triplicate, the ELLA can multi-plex biomarkers in triplicate within a single well, increasing the amount of data that can be generated from clinical samples. |
Contact: Valeria Zarate
Location: The Providence VA Medical Center »See on the MapAffiliated Centers: IDeA COBRE Center for CardioPulmonary Vascular Biology at Ocean State Research Institute
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Analyzer - Cell Metabolism | Seahorse Biosciences - XF Extracellular Flux Analyzer | XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. XF Technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well. |
Sensing equipment - Electrical Cell Impedance Sensing (ECIS) | Applied BioPhysics, Model 100 and Model ECIS Z Theta | Measures cell impedance using altering currents across cell monolayers and measuring the change in electrical potential. Impedance is calculated using Ohm's law. |
Behavioral Phenotyping - Hypoxia Chamber | BioSpherix Rodent Hypoxia Chamber | The A-Chamber works with a large range of BioSpherix Ltd. gas controllers. These controllers continuously monitor atmospheric condition, ensuring humane conditions as well as experimental integrity for Hypoxia studies. |
Imaging System - Slide Scanner | Aperio ScanScope CS Digital Slide Scanner CS | Brightfield 5 slide scanner with two workstations with Aperio brightfield imaging analysis software. |
Imaging System - Slide Scanner | Aperio Scan Scope FL Fluorescent Digital Slide Scanner | 5 slide scanner with Indica Labs fluorescence imaging analysis software. |
MPVS Ultra Foundation System | ADInstruments | High quality data acquisition device engineered to record precise, reliable, consistent data for research. Together with Millar rodent PV catheters and the LabChart software, the system offers state-of-the-art cardiac hemodynamics and functional measurements across species. |
Evaporator | Buchi RotaVapor R215 Evaporator | Rotary evaporator for removing solvents. |
Imaging System - Echocardiograph | VisualSonics, Vevo 2100 Imaging System | A high-frequency, high-resolution digital imaging platform with linear array technology and Color Doppler Mode. The Vevo 2100 Imaging System enables in vivo visualization, assessment, and measurement of anatomical structures and hemodynamic function in longitudinal imaging studies for small animal and zebrafish phenotyping. |
Channel Cycling Metabolic System | OmniTech ElectronicsModel #80 AP4RAT AccuPacer 4 Channel Rat Treadmill and #75 MS8 8 | For rodent cardiopulmonary exercise testing |
Microscope | Nikon Ni-E upright research microscope | Capture both phase, DIC, brightfield and fluorescence images at high magnification with enhanced features such as Z-stack imaging and multi-channel |
Spectrophotometer | NanoDrop | Measures the concentration of peptides, DNA and RNA, purified protein, nanoparticles, perform protein assays and optical density measurements |
MicroCT | Perkin Elmer | State-of-the-art microCT Imaging System offering longitudinal in vivo imaging as well as ex vivo sample scanning with high speed, high resolution, and low dose scans across multiple species (zebrafish, mouse, rat, guinea pig, rabbit). |
High Resolution O2k-FluoRespirometers | Orobos Instruments | Dynamically measure metabolic flux (rates) and therefore provide an integrate measure of the dynamics of mitochondrial complex coupled metabolism pathways and function in muscle fibers, cells, or mitochondria. With the fluorescent package, it also allows simultaneously measure mitochondrial H2O2 generation or ATP production. |
Imager and Microplate Reader | BioTek Cytation 5 microplate reader | Imaging plate reader; 5 colors (brightfield, GFP, RFP, DAPI, Cy5) 4x, 10x, 20x objectives. |
Imaging system - Laser Doppler | Moor instruments, laser Doppler imaging system | Laser Doppler Imaging examines blood flow non-invasively. Used to examine blow flow in capillary beds in response to pulmonary hypertension, exercise, and/or ischemia/reperfusion. |
Cell Isolation and Tissue Culture Core | Service | The function of the Cell Isolation and Tissue Culture Core is to provide efficient, economical, and reproducible isolation of pulmonary and cardiac endothelial cells, fibroblasts, vascular smooth muscle cells, epithelial cells, macrophages; propagation, and phenotypic characterization and function. |
Organ Function Core | Service | To provide measurement of heart, lung, and vessel function, in vivo angiogenesis, and transient gene manipulation in vivo. The COBRE Core will provide expertise and state of the art equipment for assessing heart, lung, and vessel function, in vivo angiogenesis, and transient gene manipulation in vivo. The Cell/Organ Core has been measuring heart and lung function using echocardiogram and Flexivent™ methodological approach. |
Respiratory Core | Service | The mission of the Respiratory Core is to develop centralized systems for cardiopulmonary structural/functional assessments and mitochondrial function analysis, with the goal to enhance the productivity and the research impact of CPVB COBRE investigators and others in the state of Rhode Island. We are committed to providing reproducible, economical, and high-quality services. We offer consultation on experimental design, provide cutting edge technological approaches and high-end instrumentation, generate and analyze data. |
Contact: Pam Borek
Location: Coro West, Suite 309 »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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STAR COBRE for Stress, Trauma, and Resilience | Clinical Research Resources, Stress, Trauma, and Resilience | The STAR COBRE aims to foster transdisciplinary collaborations within Brown University, to provide training opportunities for researchers and clinicians who want to address stress and trauma, to enhance partnerships with local and community stakeholders, and to affect policy at the local, national, and international levels. |
Technology, Assessment, Data, and Analysis Core (TADA) | Clinical Research Resources | The TADA Core will offer unique expertise in leveraging technology for observational, laboratory, and intervention studies critical for (1) obtaining ecologically-valid data on stress exposures, behavioral and physiological responses with high temporal resolution, (2) novel approaches to digital phenotyping, and (3) electronically-delivered adaptive interventions. |
Community Collaborative Core | Clinical Research Resources | The Community Collaborative Core will support the overall goals of the STAR COBRE by building critical infrastructure to support clinically significant and highly innovative research with vulnerable children and adults with stress and trauma histories. |
Contact: Heather Wunschel
Location: Lifespan - Rhode Island HospitalAffiliated Centers: IDeA COBRE on Opioids and Overdose at Rhode Island Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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IDeA COBRE on Opioids and Overdose | Clinical Research Resources, Opioids and Overdose | The COBRE aims to develop and sustain a vibrant interdisciplinary center of research excellence on opioids and overdose. The COBRE will support the selection, mentorship, and career development of junior investigators performing research in the area of opioids and overdose. The Center will bring together an interdisciplinary group of accomplished senior scientists, promising junior investigators, and distinguished advisors and collaborators to work closely together to establish a center of excellence in an area of clinical research that is of critical public health importance. |
Data and Research Methods Core Data and Research Methods Core | Service | The Data and Research Methods (DRM) Core provides study design and methodological expertise, delivers data management resources, offers data visualization services, and addresses analytical needs shared by investigators who lack access to these resources within their own research groups. |
Translational and Transformative Core Translational and Transformative Core | Service | The Translational and Transformation (T2) Core provides technical assistance in the execution of translation and implementation of science research and supports the dissemination of innovative findings to transform care for opioid addiction and overdose. |
Community Engaged Research Core Contact: Special Populations Core | Service | The Community Engaged Research Core (CERC) provides infrastructure to recruit and retain vulnerable and underserved individuals, builds capacity in the ethical conduct of research with vulnerable populations, and serves as a centralized resource for training and outreach regarding the conduct of research among individuals most impacted by overdose and opioid-related harms. |
Contact: Lei Wei, PhD
Location: The Coro West Building »See on the MapAffiliated Centers: IDeA COBRE Center for Skeletal Health and Repair at Rhode Island Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Microplate Reader Contact: Cherie Charbonneau | Molecular Devices SpectraMax M2e plate reader | Features top and bottom read capability for absorbance, fluoresence, luminescence, and time-resolved fluorescence. |
Oven - Hybridization Oven Contact: Cherie Charbonneau | Fisher Scientific Isotemp hybridization chamber | Temperature range of +7C to 85C. Includes a rotisserie. |
Incubator - Shaker Contact: Cherie Charbonneau | Lab Companion SI-600 Incubated Shaker | Bacterial culture shaker with a temperature of ambient to +60C. |
Luminometer Contact: Cherie Charbonneau | Promega Glomax 20/20 luminometer with printer | Ultrasensitive luminescence detection in a single microcentrifuge tube (1.5ml) format. |
PCR Thermal Cycler Contact: Cherie Charbonneau | Bioer Life ECO thermal cycler | This thermal cycler has a 96 well format with a 2C/sec ramp rate. |
PCR Thermal Cycler Contact: Cherie Charbonneau | Applied Biosystems ProFlex thermal cycler | This thermal cycler has a 3x32 well format that allows for 3 separate programs to be run. The ramp rate is 6C/sec. |
PCR Thermal Cycler Contact: Cherie Charbonneau | Applied Biosystems Veriti thermal cycler | This thermal cycler has a 96 well format with 3.9C/sec ramp rate. |
Imaging System - Gel Imager Contact: Cherie Charbonneau | LI-COR Odyssey Classic Infared Imaging system | This near-infared fluorescent detector can be used for quantitative western blots, gel shift assays, coomassie stains, tissue section and small animal imaging. |
Imaging System - Gel Imager Contact: Cherie Charbonneau | UVP GelCam 310 Bioimaging digital gel documentation system | High resolution 2.0 GelCam 310 CCD with manual zoom lens for fluorscent and colorimetric gel imaging. EtBr filter included. |
PCR Thermal Cycler Contact: Cherie Charbonneau | MJ Opticon 2 real-time PCR system. | This 96-well system accepts low-profile tubes or plates. There are two fluorophore channels with emission spectra in the 523-543 nm range and 540-700nm. |
Incubator Contact: Cherie Charbonneau | (4) NuAire IR water-jacketed CO2 incubators (NU-8500) | This incubator provides an optimal in-vitro growth environment for both cell and tissue culture while minimizing dessication through a closed loop HEPA filtration system. |
Biological Safety Cabinet Contact: Cherie Charbonneau | (4) NuAire Bio Safety Cabinets (Class II, Type A/B3) | These biological safety cabinets reduce exposure to airborne biological or particulate chemical agents in low to moderate risk-hazard research and/or drug preparations. |
Centrifuge Contact: Cherie Charbonneau | Sorvall Legend X1R centrifuge | Process samples for diverse laboratory applications with rotors for 1.5ml eppendorf tubes, 15, and 50ml conical tubes. Refrigerated with a max speed of 152000 rpm. |
Autoclave Contact: Cherie Charbonneau | Amerex HVE-50 top loading autoclave sterilizer | This is a 50L, self-contained, portable, top-loading autoclave with auto-exhaust and warming cycles. |
Microscope Contact: Cherie Charbonneau | Nikon Eclipse TS-100F Inverted Microscope | This phase contrast microscope used for tissue culture is also equipped with FITC and TRITC filters to capture fluorescent images in SPOT imaging software. |
Microscope Contact: Cherie Charbonneau | Nikon Eclipse 90i Upright Research Microscope | This microscope is equipped with two cameras (color and monochromatic) and is ideal for brightfield and/or epi-fluorescent images of whole tissues etc with 4x, 10x, 20x and 40x dry objectives. The filter cubes available are DAPI, FITC, and TRITC. |
Fluorescence Activated Cell Sorter (FACS) Contact: Cherie Charbonneau | Becton Dickinson - Accuri Flow Cytometer | This system is equipped with a blue and red laser, two light scatter detectors, and fluorescence detectors with optical filters optimized for FITC, PE, PerCP, and APC. The user does not need to adjust detector voltages and there is a wide dynamic range of digitally collected data (7.2 decades). |
Bioanalyzer Contact: Cherie Charbonneau | Invitrogen Luminex 200 with xMap technology | This is a versatile, efficient standard in multiplexing with the ability to use magnetic or polystyrene beads on up to 100 targets/sample. |
pH Meter Contact: Cherie Charbonneau | Orion pH meter 920 | pH range of -2 to 19.99 and oxygen range of -2 to 19.99ppm. Autocalibration buffers of 4.01, 7, and 10.01. |
pH meter Contact: Cherie Charbonneau | Hanna Instruments 2209 | This pH meter features manual pH calibration and temperature compensation. |
Microtome Contact: Cherie Charbonneau | Thermo Scientific Shandon Finesse 325 manual microtome | With a lightly balanced flywheel it is ideal for routine paraffin sectioning. |
Sample Preparation - Cutter Contact: Cherie Charbonneau | Buehler-Isomet Cutter | The IsoMet Low Speed Cutter is a precision sectioning saw designed for cutting various types of materials with minimal deformation. Its low kerf loss capability and great versatility in holding all types of sample shapes and configurations, gives today's laboratory a precision sectioning tool capable of cutting viturally any material including brittle or ductile metals, composites, cements, laminates, plastics, electronic devices and biomaterials. The Cutter is equipped with a diamond studded carborundum wheel and Buehler Ecomet 4 grinder/polisher with Automet 2 powerhead. |
Imaging System Contact: Cherie Charbonneau | PerkinElmer FMT 4000 Fluorescence Tomography System. | The FMT 4000 fluorescence tomography imaging system provides the greatest utility of the FMT Systems with the ability to quantitate up to four fluorophores simultaneously. The system comes with four excitation laser channels (635, 680, 750, and 790 nm) and is useful for a range of applications including oncology, inflammation, and cardiovascular disease. It is ideal for researchers who are well-versed in 3D fluorescence tomography and require the widest range of in vivo applications and imaging agents. |
Imaging System Contact: Cherie Charbonneau | Faxitorn Pre-clinical imaging system | Ultra-high resolution x-ray imaging is an important tool in investigating a variety of disease states. From metastatic cancer to arthritis and osteoporosis, small animal models are commonly used. Both in vivo and ex vivo planar imaging is used to screen, track, and evaluate structural changes in these studies. Cabinet x-ray systems allow researchers to take images directly in the vivarium to protect the integrity of the colony. These planar imaging systems are less expensive, subject animals to lower doses, and provide faster results than microCT systems. |
Incubator Contact: Cherie Charbonneau | GQF 1502 Sportsman egg incubator | This is a complete incubator and hatching system with 3 automatically turned racks. It has an accurate digital thermostat with LCD of humidity and temperature. |
Homogenizer Contact: Cherie Charbonneau | Fisher Scientific, Powergen 125 Tissue Homogenizer | Process volumes from 0.03 to 100mL. Variable speed control to 30,000rpm for efficient cell disruption. Disposable plastic generators eliminate sample cross-contamination. Flat-bottom stainless-steel generators process liquid or soft tissues. |
Microscope Contact: Cherie Charbonneau | Olympus SZ61 Zoom Stereomicroscope | Greenough design with two optical paths inclined at an inward angle. Magnification range 0.67X to 4.5X; zoom ratio 6.7:1; FN 22. Working distance of 100mm; observation tube angle 45 degrees; right and left eyepiece tubes interlocked; interpupillary distance adjustment 50-76mm; forward positioned zoom adjustment knobs with zoom magnification graduations. With 0.5x cmount for documentation. |
Chemical Hood Contact: Cherie Charbonneau | Thermo Scientific Hamilton SafeAire II | Chemical hood and ultra-high vacuum system for organic synthesis |
Evaporator Contact: Cherie Charbonneau | Buchi R-124 Rotavapor | Rotary evaporator comes complete with a 45 degree water condenser and a 1L condensing and evaporating flask. |
Sample Preparation - Circulator Contact: Cherie Charbonneau | VWR Recirculating Chiller, Model 1160A | Stable cooling for many common heat removal applications such as rotary evaporators and vacuum systems (-10 to 40C). |
High Performance Liquid Chromatography (HPLC) Contact: Cherie Charbonneau | Agilent Technologies, 1260 Infinity HPLC system | A maximum pressure of 600 bar to utilize smaller particle size columns for higher resolution and faster separations. |
Disrupter Contact: Cherie Charbonneau | Qsonica 700W sonicator with 5.5" cup horn | This sonicator has full amplitude control from 1-100%. |
Imaging System Contact: Cherie Charbonneau | Xtreme back illuminated 4MP with x-ray for small animal imaging | High sensitivity luminescence, fluorescence, radioisotopic, and radiographic imaging for up to 5 mice at a time. Isofluarne and temperature controlled chamber for the mice. |
PCR Thermal Cycler - Digital Contact: Cherie Charbonneau | RainDrop Digital PCR by RainDance | The RainDrop is composed of a Source instrument that can generate 10million droplets/sample and the Sense instrument, which can detect whether the PCR reactions are positive or negative for your fluorophore. Multiplexing is possible for the 8 samples that are run at a time on the microfluidics chip. |
PCR Thermal Cycler Contact: Cherie Charbonneau | Bio-Rad CFX96 Real-Time PCR system | This real-time PCR instrument allows for up to 5-target multiplexing in sample volumes as small as 10ul. It also has a thermal gradient feature in a 96-well format. |
Spectrophotometer Contact: Cherie Charbonneau | Thermo Scientific NanoDrop 2000c | This is a full spectrum, UV-Vis spectrophotometer used to quantify and assess the purity of DNA, RNA, protein, and more at sample volumes as low as 0.5ul. |
Bioreactor Contact: Cherie Charbonneau | Flexcell FX5000 Tension System | This is a computer-regulated bioreactor that uses vacuum pressure to apply either cyclic or static strain to cells cultured on flexible-bottomed culture plates. |
Sample Preparation - Freeze Dry System Contact: Cherie Charbonneau | Labconco FreeZone Plus 2.5L Cascade Benchtop Lyophilizer | This system is for light sample loads with low eutectic points. It can remove up to 1.8L of water in 24hrs and holds 2.5L of ice before defrosting is required. |
Contact: Arunasalam Navaraj, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Advise investigators with experimental design for in vivo imaging applications, provide access to instrumentation and training. Assist investigators with in vivo mouse, organ or cellular imaging, probe selection, antibody labeling, image analysis and presentation. Provide support for grant submissions. |
Contact: Pamela Poethke, RN
Location: Fain Building »See on the MapAffiliated Centers: AIDS/HIV Immunology Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Research - Infectious Diseases Clinical Research Resources Contact: Karen Tashima | Clinical Trials Support, HIV and HCV | The Miriam Hospital clinical trials unit offers the newest treatments for HIV and hepatitis C (HCV) infections. The experienced research staff consists of physicians, nurses, a data manager, a regulatory assistant and laboratory personnel. The unit conducts trials offered through the AIDS Clinical Trials Group (ACTG) network, funded by the National Institutes of Health, in association with the Partners/Harvard AIDS Clinical Trials Unit. The priority of ACTG studies is to evaluate new strategies for treating HIV infection and preventing HIV complications, and to evaluate new treatments for hepatitis C. Other studies offered through our clinical trials unit include pharmaceutical studies to treat hepatitis C, prevent C. difficile infection, and test new methods of diagnosing HIV infection. |
Contact: Michael Packer
Location: Institute for Molecular & Nanoscale Innovation (IMNI), Room 220, Prince Lab »See on the MapAffiliated Centers: Instrumentation for Molecular and Nanoscale Innovation (IMNI)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Fabrication - Machine Shop | Service that provides machine shop capabilities | The Joint Engineering Physics Instrument Shop (JEPIS) is a specialized service center providing assistance in the development of experimental instruments, research projects and instructional classes. The JEPIS services are offered to faculty, students and staff on the Brown University campus, and is also available to outside academic/hospital users. JEPIS offers all of the basic machine shop capabilities, including Wire EDM and Rapid Prototype printing. JEPIS also offers CNC design consultation, hands on training and instruction through non-credit basic machine shop course and an advanced course geared toward Graduate Students. The Student Shop provides students with a strong base for their experimental studies. The JEPIS staff is available to give general advice and assistance to the Brown Students and Staff in the finding of sources for the materials and devices they require for their projects or instructional classes. Access to the facility is on a user fee basis of an hourly fee for labor plus materials. |
Contact: Robbert Creton, PhD
Location: Room 121, Sidney Frank Hall »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Software - Analysis | Software: Amira 2019 | Software for image analysis and 3D visualization |
Sample Preparation - Critical Point Dryer | Ladd Research Industries | Removes liquid from biological specimens for SEM |
Sample Preparation - Glow Discharge Unit | Emitech K100X | Adds a charge to make TEM samples hydrophilic |
Microscope - Multiphoton | Olympus FV-1000-MPE Multiphoton Laser Scanning Microscope | Deep optical sectioning of fluorescently labeled samples with SIM photoactivation |
Microscope - Fluorescence | Zeiss Axiovert 200M Fluorescence | Imaging of samples labeled with fluorescent indicators |
Microscope - Fluorescence | Zeiss Axiovert 200M Fluorescence | Imaging of samples labeled with fluorescent indicators |
Microscope - Confocal | Olympus FV3000 Confocal Microscope | Optical sectioning of samples labeled with fluorescent indicators. Equipped with a resonant scanner for fast optical sectioning |
Microscope - Confocal | Zeiss LSM 710 Confocal | Optical sectioning of samples labeled with fluorescent indicators |
Microscope - Fluorescence | Zeiss Lumar Stereo Fluorescence | Imaging of samples labeled with fluorescent indicators |
Microscope - Electron | Thermo Apreo VS Scanning Electron Microscope (SEM) for serial block-face imaging | Equipped for standard scanning electron microscopy as well as serial-block face imaging. The automated system is capable of imaging large z-stacks at isotropic 10x10x10 nm resolution. The series of images can be processed for 3D reconstruction of cells and tissues, with sufficient resolution to identify small vesicles, organelles and neural connections. |
Thermo Tundra Cryo-TEM Contact: Robbert Creton | ||
Microtome | Reichert Ultramicrotome | Ultrathin sectioning for light microscopy and transmission electron microscopy |
Microtome Diamond Knife Set | Diatome DKS | Used on Reichert microtome to cut ultrathin sections for TEM |
Sample Preparation - Sputter Coater System | Emitech K550 | Covers an EM specimen with a thin layer of conducting material |
Training and Technical Assistance - Bioimaging | Service | Service to provide Bioimaging technical assistance and training |
Microscope - Slide Scanner | Olympus VS200 | Automated slide scanner for imaging fluorescence and brightfield (e.g. H&E slides) in the Laboratories for Molecular Medicine (LMM) |
Microscope - Fluorescence | Nikon Ti2-E Fluorescence Microscope | Motorized fluorescence microscope with Perfect Focus System and software for high-content screening and 2D/3D deconvolution. |
Microscope - Confocal | Zeiss LSM 800 Confocal | Optical sectioning of samples labeled with fluorescent indicators |
Microtome | Leica EM UC7 Ultramicrotome | Biological TEM and SBF prep |
High-Content Screening System Contact: Samantha Madnick Opera Phenix High-Content Screening System Website | PerkinElmer Opera Phenix High Content Screening System | Microscope designed for high-throughput 3D imaging of fluorescent samples in microplates. |
Microscope - Fluorescence | Nikon Ti2-E Fluorescence Microscope for high-content imaging | The Nikon Ti2-E fluorecence microscope is a high-content imaging system, based on an inverted microscope and equipped for imaging standard slides, culture dishes, and microplates (e.g. 96-well plates). The microscope includes 'Perfect Focus' for autofocus applications, a Photometrics Prime 95B sCMOS camera for imaging fluorescence, a 16 megapixel Nikon DS-Ri2 color camera for imaging histology slides, a motorized stage and NIS-Elements High Content (HC) software for multiwell plate acquisition. |
Microscope - Confocal | Zeiss LSM 880 Confocal | Optical sectioning of samples labeled with fluorescent indicators |
Microscope - Slide Scanner | Olympus VS200 | Automated slide scanner for imaging fluorescence and brightfield (e.g. H&E slides) located in the BioMed Center. |
Contact: Olin Liang, Ph.D.
Location: CORO West Research Building, Suite 5.17J »See on the MapAffiliated Centers: IDeA COBRE Center for Stem Cells and Aging at Rhode Island Hospital, Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Lentivirus-mediated Stable Gene Over-expression in Cells | Service | |
Lentivirus-mediated CRISPR/Cas9 Gene Knock-out in Cells | Service | |
Site-directed Mutagenesis | Service | |
Virus Packaging | Service | |
Design and production - COVID-19 Pseudovirus | Service | |
Cell entry assays - COVID-19 Pseudovirus | Service | |
SARS-CoV-2 spike and human ACE2 constructs | Service |
Contact: Jason Machan, PhD
Location: 130 Plain Street Providence, RI 20903 »See on the MapAffiliated Centers: IDeA Advance RI-CTR (Clinical Translational Research) at Brown University, Lifespan Clinical Research Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Technical Assistance - Biostatistics, Epidemiology, and Research Design Consulting Contact: Jason Machan, PhD | Service | The Biostatistics, Epidemiology, and Research Design (BERD) Core at Rhode Island Hospital offers centralized biostatistics consultation services to clinical, basic, and translational researchers. We provide a broad array of resources during all phases of research. During planning, the core can provide high quality project/trial design, including final sculpting of specific aims and hypotheses, measure selection, power analysis, data analysis planning, and grant/proposal preparation. At the beginning of the project, the core can assist in construction of REDCap data collection tools, databases, and data management, including national archiving. We provide help navigating approvals for special technology needs, such as purchasing non-standard equipment and logistics. Throughout the trial, the core can provide decision support, assessing the impact of potential design additions or changes on final statistical inference. It can serve on or help find individuals to serve on data and safety monitoring boards (DSMB). At the conclusion of the trial, the core can assist with implementation of the data analysis plan, either by providing access to contemporary statistical analysis software and coaching, or by conducting analyses for or with investigators. During reporting/publication preparation, the core can assist in write-up, data visualization, and integration of interpretations in the broader context of the project, and help address comments from peer reviewers. |
Contact: Michael Worden, PhD
Location: Sidney Frank Hall »See on the MapAffiliated Centers: Carney Institute for Brain Science
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Imaging System - Magnetic Resonance Imaging (MRI) Scanner | Siemens 3 Tesla Prisma fit | Brain structure and function in normal and clinical populations as well as studies of other body systems, non-invasive animal imaging and materials science. |
Contact: makerspace@etal.uri.edu
Location: Room 165, Carothers Library & Learning Commons »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Imaging System - 3D Scanner | EinScan Pro 2X | |
Tools - Router - computer numerical control (CNC) | Inventables X-Carve | |
Cutter - Laser | GCC Laserpro Spirit LS | |
Imaging System - Audio|Visual - VR Headsets | HTC Vive Pro (3); Oculus Quest 2 (2) | |
Sewing Machines/ Embroidery | Brother XM2701 Sewing Machine (2), Brother PE-770 Embroidery System | |
Imaging System - 3D Printer | Makerbot Replicator+ (2); Dremel 3D20 (9); TAZ Workhorse; Formlabs (2) | |
Computer - Tablets | IPad 7th Gen (4), Microsoft Surface | |
Imaging System - Camera | Sony Alpha a6400 Mirrorless Digital Camera (with a tripod and accessories) | |
Computers | DELL high-performance PCs (4), Alienware PC (3) | |
Vacuum Former | Vaquform DT2 | |
Cutter - Vinyl | GCC Expert Pro Vinyl Cutter, Cameo Silhouette (2) |
Contact: Alexander Margulis, PhD
Location: 241 Francis Avenue »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Workstation - Laboratory and office space Mansfield Bio-Incubator 2020 Rates | Mansfield Bio-Incubator | Mansfield Bio-Incubator is a non-profit organization whose mission is to facilitate and assist the creation, growth, and success of the next generation of biotech companies. Our incubator assists these companies and nurtures entrepreneurial and economic development in the community by maintaining affordable lab and office space, services, training, mentoring, and a network of professional advisors. Shared and private office and lab space are available, with additional space coming online in 2021. More information on the membership rates can be found here. https://www.bioinc.org/membership-rates-2 |
Chemical Hood | Labconco Protector Premier Fume Hood | Suitable for providing personal protection needed for general chemistry applications involving fumes and vapors. |
Biological Safety Cabinet | Labconco Purifier Logic Series Biological Safety Cabinet | Provide personnel, product and environmental protection from hazardous particulates and agents that require biosafety level 1 or 2 containment. |
Incubators (2) | VWR Symphony CO2 incubator | CO2 incubators designed to maintain adequate interior moisture to prevent the drying out of cultures. |
PCR Thermal Cycler | Illumina Eco real-time PCR system | The Eco Real-Time PCR System offers life science researchers a full-featured real-time PCR system at an attractive price. Its features include: Four-color multiplexing High Resolution Melt (HRM) Fast PCR cycling: 40-cycle PCR in 40 minutes User-friendly, MIQE-compliant software. |
Mass Spectrometer | Agilent LC/MSD VL mass spectrometer | The Agilent 1100 Series LC/MSD offers you the sensitivity, selectivity, and depth of molecular information inherent in mass spectrometry—in a package that is rugged, reliable, and surprisingly easy to learn, operate, and maintain |
Microplate Reader - Fluorescence | Fluoroskan Ascent | Measures sample fluorescence Excitation wavelengths 320-700 nm, emission 360-700 nm |
High Performance Liquid Chromatography (HPLC) | Agilent 1100 HPLC system | |
BioBank - Biospecimen Banking - Freezer | NuAire -85C Ultralow Temp Freezer | Sample storage |
BioBank - Biospecimen Banking - Freezer | Kenmore -20C Freezer | Sample storage |
BioBank - Biospecimen Banking - Refrigerator | 4C Glass Door | Sample storage |
Luminometer | Digene DML 2000 luminometer | The DML 2000 instrument is a luminometer that automates amplified chemiluminescent signal detection and results reporting for Hybrid Capture 2 (HC2) assays, |
Contact: Dawn Outram
Location: Aquarium Building »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Sensor - Oxygen Sensor | PreSens | Water phase analysis |
Analyzer - Dissolved Gas | Bay Instruments Membrane Inlet Mass Spectrometer- Dissolved Gas Analyzer | Mass Spectrometer: Quantifies dissolved gases |
Analyzer - Multiparameter Water Analyzer | YSI Professional Plus Hand Held Multiparameter Water Meter | Measures temperature, salinity, dissolved oxygen (DO), and pH |
Analyzer - Nutrient | Lachat QuickChem 8500 Flow Injection Nutrient Auto Analyzer | Measures dissolved nutrients in water, Ammonia, Phosphate, Nitrite, Nitrate, and Silicate |
Aquatic Culture - Algae and Live Feed Room Contact: Ed Baker | Various manufacturers | K-walls, small rearing tanks for live feed, flowing seawater and freshwater |
Aquatic Culture - Phytoplankton Culture Wheels Contact: Ed Baker | Various manufacturers | Motorized and water driven, clear plexiglass, ambient and experimental temperature control, can accommodate culture bottles of differing sizes (1-3L) |
Aquatic System - Dechlorination System - Freshwater Contact: Ed Baker | Various manufacturers | 2 Freshwater dechlorination tanks |
Disrupter | Biospec Mini Bead Beater | Shakes tubes with beads to disrupt cells, DNA , RNA |
Disrupter | Branson Digital Ultasonic Liquid Sonifier | Disrupts cells |
Workstation - Environmental Chamber Contact: Ed Baker | EnvironAir Environmental Chamber | 100 sq ft Temperature control, Two range 4-24 C, Two range -4-24 C (to simulate polar conditions), On generator back up and emergency monitoring systems. (4 Units) |
Aquatic System - Flowing Seawater System Contact: Ed Baker | Various manufacturers | Raw-Ambient temperature and Filtered- Chilled, Heated, Ambient. UV treated, air supply, emergency monitoring systems |
Fluorescence Activated Cell Sorter (FACS) | Becton Dickinson (BD) Biosciences Influx Flow Cytometer and Cell Sorter | Measures light scatter and fluorescence of cells. Sorts cells based on measured properties. |
Fluorometer | Satlantic Fluorescence Induction Relaxation Fluorometer (FIRE) | Measures variable fluorescence in phytoplankton |
Fluorometer | Turner Designs 10-AU (Chlorophyll) Field Fluorometer | Measures extracted or in vivo chlorophyll fluorescence of samples, Field-portable, can be set up for continuous-flow monitoring or discrete sample analyses |
Fluorometer | Life Technologies Qubit Fluorometer | Quantifies DNA, RNA, and protein |
Biospecimen Banking - Freezer - Walk-in Contact: Ed Baker | -20 C Walk-In Freezer | 185 sq ft Long term storage, On generator back up and emergency monitoring systems |
Homogenizer | Polytron Homogenizer PT-10-35-GT | Breaks down plant and animal tissue for analysis, and performs homogenization, dispersion, mixing, and prepares emulsions |
Cell Counter | Sizing and counting of particles suspended in liquid samples | Beckman Coulter Multisizer 4 |
Imaging System - Camera | Olympus EP50 Microscope Camera w/ HDMI Monitor | Captures Microscope video and still images (C-mount) |
Analyzer - Fluid Particle Counter and Imager | Fluid Imaging Technologies FlowCam | Imaging Particle Analysis: counts and records images of particles in liquid samples |
Incubator | Percival Incubator/Growth Chamber | Temperature and light controlled incubator for environmental applications |
Microplate Reader | Molecular Devices Spectramax M5 | Measures Fluorescence Intensity, Absorbance, Time Resolved Fluorescence, Fluorescence Polarization and Luminescence in cuvettes and multiple sized microplates. |
Microscope | Nikon inverted Microscope | Microscopy. Works with Olympus EP50 Camera |
Microscope | Olympus SZX16 Stereo Microscope | Microscopy. Works with Olympus EP50 Camera |
PCR Thermal Cycler | Eppendorf Master Cycler Pro | Replicates DNA |
PCR Thermal Cycler | Stratagene QPCR Mx3000P | Analysis of gene expression, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Quantitative PCR Filters- FAM/Syber Green, Rox, Hex, CY3, CY5 |
Photoperiod controller Contact: Ed Baker | Various manufacturers | Wattstopper controller(Blount), Various individual timers |
Pier Access Contact: Ed Baker | Access to Graduate School of Oceanography pier | Access to pier for deployment, collection, testing. Water depths 18-15 feet to intertidal zone |
Radiometer | Biospherical Instruments Quantum Scalar Laboratory (QSL) Radiometer - PAR Light Meter | Measures Photosynthetically Active Radiation (PAR) |
Incubator - Shaker | Thermo Max-Q Orbital Shaker Table | Orbital shaker with 18x24 in platform and variety of clamps |
Incubator - Shaker | Thermo Max Q Incubated Shaker | Heated incubated shaker |
Spectrometer | Ocean Optics Jaz Spectrometer | Optical sensing instrumentation that measures light wavelengths. Its unique features and expandable platform makes it uniquely suited for field applications, remote sensing, process flow and quality assurance. |
Spectrophotometer | Thermo Scientific NanoDrop 1000 | Microvolume UV-Vis spectrophotometer that quantifies DNA, RNA, and protein from small 1-2 microliter sample size |
Aquatic System - Laminar Flow Tank Contact: Ed Baker | Laminar Flow Tank | 1000 gal, Seawater or Freshwater, Multiple speeds with precision control. Experimental chamber visable from 5 sides |
Aquatic System - Mesocosm Tank Contact: Ed Baker | Various manufacturers | 10, 5 ft deep outdoor mesocosms, with available raw ambient seawater flow |
Vacuum Manifold Contact: Rodrigue Spinette | Vacuum Filter Manifold | Allows for filtration of 5 ports simultaneously, 25 and 47 mm filter cups available |
Worksation - Marine General Wet Lab Contact: Ed Baker | Various manufacturers | 3 Buildings, 7,000 sq ft indoor, and nearly unlimited outdoor space, Multiple insulated tank sizes, various sized aquaria, possible to perform simultaneous replicates |
Workstation - Marine Pathology and Transgenics Laboratory and Systems Contact: Ed Baker | Various manufacturers | Ozone treated effluent and separate effluent drain |
Microscope | Nikon Eclipse Ni epifluorescence microscope | Epifluorescence and phase microscopy with digital camera system |
Gas Chromatograph - Mass Spectrometer (GC - MS) Contact: David Adelman | Agilent Technologies 7890B/5977A GC/MSD | Identifies and enumerates chemical constituents of samples |
Imaging System - Gel Imager | Fluorescence gel imaging system | Carestream Molecular Imaging Gel Logic 212 Pro |
Fluorometer | Turner Designs Trilogy | Measures extracted or in vivo chlorophyll fluorescence of samples, Field-portable |
Contact: Soumen Mallick
Location: GeoChemistry, GC040 »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Mass Spectrometer Contact: Alberto Saal | Thermo Scientific Multi-collector inductively coupled plasma mass spectrometer, ICP-MS, Neptune Plus | This is a multi-collector ICP-MS equipped with nine Faraday cups and eight ion counters, which allows us to monitor multiple masses simultaneously providing precise isotope ratio measurement. |
Mass Spectrometer Contact: Alberto Saal | Thermo Scientific Quadrupole inductively coupled plasma mass spectrometer, ICP-MS, X-series-2 | This is a single collector ICP-MS that allows us to measure precise elemental composition at sub-ppb level concentration. |
Analyzer - Laser Ablation Contact: Alberto Saal | Photon-machines Analyte G2, 193 nm excimer laser ablation | This 193 nm wave-length excimer laser allows us to perform in-situ analysis on a solid sample with a beam diameter as small as 3µm. To determine the elemental abundances the laser is coupled with the single collector inductively coupled plasma mass spectrometer ICPMS and to determine the isotope ratios the laser is coupled with multi collector ICPMS. |
Workstation - Clean lab, metal free Contact: Alberto Saal | Pico Trace Clean Lab | This Clean Lab is equipped with Millipore water filtration system that provides greater than 18MΩ Milli-Q water. The working stations in this Clean Lab meet ISO class 4 standard (equivalent to FS class 10) allowing us to perform ultra-low blank chemical analysis. |
Contact: Lisa Chen
Location: MacMillan Hall »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Gas Chromatograph - Mass Spectrometer (GC - MS) | Hewlett-Packard (Agilent) 6890 GC/5973 MSD | This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass range 450 Da). |
Liquid Chromatography - Mass Spectrometer (LC - MS) | Agilent 6530 Mass QTOF | This system consist of Agilent 1260 infinite HPLC and Agilent 1260 infinite nanoglow LC/chipcube nanoelectrospray MS interface, and 6530 Accurate Mass Q-TOF mass spectrometer. This instrument can detect and identify compounds in amounts smaller than two trillionths of a gram (2 picograms), making the Agilent 6530 a powerful tool for food safety, toxicology and other measurements where minute amounts of compounds must be identified. |
Mass Spectrometer | Thermo LXQ linear ion trap with Surveyor HPLC system | The LXQ has capabilities similar to the conventional 3D ion trap such as LCQ Deca. It is based on linear ion trap technology that has faster cycle time. It is capable of data-dependent LC/MSn analysis. |
High Performance Liquid Chromatography (HPLC) - Analytical and preparative HPLC | Agilent 1260 Infinity LC | The 1260 HPLC is used primarily for analytical and semi-prep scale HPLC separation and purification of components in chemical mixtures. This system consisted a quaternary pump, autosampler, thermostatted column compartment, diode array detector (190 – 950 nm), and fraction collector. |
Liquid Chromatography - Mass Spectrometer (LC - MS) | Agilent 6230 Mass QTOF | This 6230 time-of-flight LC-MS system couples accurate mass TOF with the 1260 Infinity II liquid chromatography, which is equipped with dual electrospray (ESI) ion source and Multiple Wavelength UV Detector (MWD). This TOF LC/MS achieves resolution up to 20,000 (FWHM) and sub 1-ppm mass accuracy. It is capable of screening, profiling and identification of small molecules, large biologic compounds, and everything in between. |
Mass Spectrometer | Bruker Autoflex Speed Linear (L) Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometer (MALDI-TOF MS). | The Bruker autoflex Speed is a linear time-of-flight (TOF) mass spectrometer equipped with a matrix-assisted laser desorption ionization (MALDI) ion source. This instrument is routinely used for analysis of organic molecules, natural products, and macromolecules, including recombinant proteins, oligonucleotides, polysaccharides and synthetic polymers. |
Gas Chromatograph - Mass Spectrometer (GC - MS) | Agilent 5977A GC/MSD System | This system is suitable for separation of complex mixtures by gas chromatography (GC) and identification by mass spectrometry. It is limited to low molecular weight and volatile molecules (mass range 1050 Da). |
Contact: David Silverberg
Location: Laboratories for Molecular Medicine, Room 539 »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Histology/Tissue Processing - Tissue Sample Processing and Embedding | Service | Paraffin Processing and Embedding- automated and manual, Plastic Processing and Embedding, TEM and SEM Processing and Embedding, Frozen Tissue-Embedding in OCT Block, Sample Preparation for Serial Blockface Imaging |
Histology/Tissue Processing - Sectioning | Service | Paraffin sectioning, plastic sectioning, Frozen/Cryostat prep and sectioning, TEM ultrathin sectioning, Vibratome, Serial and Step sectioning |
Paraffin Embedding Workstation | Leica EG1150C Paraffin Embedding Center | Paraffin embedding |
Microtome | Leica RM2265 Automated Microtome | Paraffin and plastic sectioning |
Cryostat | Leica CM3050S Cryostat | Sectioning of fixed and unfixed frozen samples |
Vibratome | Leica VT 1000S Vibratome | Sectioning of fixed samples |
Biospecimen Banking - Freezer | Revco -80 degree C Freezer | Temporary Sample storage |
Microscope | Nikon Eclipse 50i light microscope | Collaborative analyses of histological slides and digital imaging capability |
Histology/Tissue Processing - Staining | Service | Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS), Masson's Trichrome, Special Stains |
Histology/Tissue Processing - Immunolabelling | Service | Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Immunogold, TUNEL Assay |
Contact: Evgeny Yakirevich, MD
Location: Aldrich Building , 6th Floor, Rhode Island Hospital Campus »See on the MapContact: Jinping Luo, PhD
Location: Laboratories for Molecular Medicine »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Biological Safety Cabinet | NuAire Safety Cabinets Class 2 Type A/B3 | Transgenics and gene targeting safety cabinets (2 units) |
Centrifuge | Thermo IEC Centra CL3R Refrigerated Centrifuge | Transgenics and gene targeting refrigerated centrifuge |
Electroporation System | Bio-Rad Gene Pulsar II | Transgenics and gene targeting electroporation system |
Biospecimen Banking - Freezer | Custom BioGenic Systems Liquid Nitrogen (LN2) Freezer | Transgenics and gene targeting liquid nitrogen freezer |
Biospecimen Banking - Freezer | Thermo Fisher Scientific -80 C | Transgenics and gene targeting -80 C freezer |
Biospecimen Banking - Freezer | Frigidaire -20 C Freezer | Transgenics and gene targeting freezer |
Transgenics and Gene Targeting - ES Cell Gene Targeting System | Service | Gene targeting services through transfection and microinjection of murine embryonic stem (ES) cells to produce germline knockout (KO) or knockin (KI) mice. |
Incubator | NuAire CO2 water jacketed Incubators | Transgenics and gene targeting incubators (3 units) |
Spectrophotometer | ThermoFisher Scientific NanoDrop Lite | Measures nucleic acid and protein concentration |
Cell Manipulator - Microinjection system | Eppendorf FemtoJet Microinjection System | Transgenics and gene targeting pronuclear injection system |
Micromanipulator | Eppendorf Transferman NK 2 Micromanipulators | Transgenics and gene targeting micromanipulators (2 units) |
Microscope | Nikon Eclipse TE200 Inverted Microscope | Transgenics and gene targeting microinjection microscope |
Microscope | Nikon Eclipse TS100 (cell culture scope) | Transgenics and gene targeting cell culture scope |
Microscope | Nikon SMZ1500 Zoom Stereomicroscopes | Transgenics and gene targeting dissecting microscopes (2 units) |
Microscope | Nikon SMZ800 Zoom Stereomicroscopes | Transgenics and gene targeting surgical microscope |
Transgenics and Gene Targeting - CRISPR/Cas9 Design and Reagent Preparation | Service | Service to provide consultation, design a CRISPR model, synthesize sgRNAs and prepare microinjection mix |
Transgenics and Gene Targeting - CRISPR/Cas9 Donor Construction/Preparation | Service | Service to construct a donor DNA for a CRISPR model.Knock-In (KI, >80bp), Knock-Out (KO), Conditional Knock-Out (cKO - two steps), Point Mutation (PM) |
Transgenics and Gene Targeting - Transgenesis by Random Integrations | Service | Service to provide custom services related to microinjection, ES cell culture, mouse surgery, or mouse reproduction. The core facility will provide services and/or training related to our capabilities that are not specifically covered by our service list. These services are available for an hourly fee. Examples include: production of MEFs (murine embryonic fibroblasts), polar body biopsy, reproductive surgery, blastomere injection, frozen embryo shipment, training |
Transgenics and Gene Targeting - Mouse Line Recovery from Cryopreservation | Service | Service to thaw and implant previously cryopreserved mouse embryos From frozen embryos or frozen sperm. |
Transgenics and Gene Targeting - Cryopreserved Mouse Embryo Storage | Service | Service to store cryopreserved mouse embryos in liquid nitrogen (LN2) |
Transgenics and Gene Targeting - Derivation of Mouse Embryonic Stem (ES) cell lines | Service | Service to produce embryonic stem (ES) cells from an existing mouse line key words: murine embryonic stem cells |
Transgenics and Gene Targeting - Chimeric Mouse Production | Service | Service to produce chimeric mice for analysis through microinjection of murine embryonic stem (ES) cells into blastocyst stage embryos. |
Transgenics and Gene Targeting - Mouse Colony Expansion | Service | Service to use superovulation and surgical implantation to produce large cohorts of age matched mice for experimentation or to quickly expand a mouse colony |
Transgenics and Gene Targeting - Mouse Embryo Cryopreservation | Service | Service to cryopreserve valuable mouse lines for storage or shipment to other investigators. Heterozygous embryos or Homozygous embryos |
Transgenics and Gene Targeting - Mouse In vitro Fertilization (IVF) | Service | Service to perform in vitro fertilization of mouse embryos using fresh or frozen sperm. |
Transgenics and Gene Targeting - Rederivation of Mouse Lines | Service | Service to rederive mouse lines obtained from a source other than a Brown University approved vendor. Rederived mouse lines may be housed in any campus mouse facility. |
Transgenics and Gene Targeting - Embryo Microinjection/Electroporation | Service | Service to perform pronuclear microinjection of 0.5 dpc mouse embryos to produce transgenic or CRISPR founders on an FVB/N or C57/BL6 background. Custom backgrounds also available. |
C1000 Touch Thermal Cycler | Bio-Rad | Transgenics and gene targeting reagents preparation or PCR for genotyping |
Micropipette puller, Model P-97 | Sutter Instrument co. | Prepare microinjection holding or injection glass pipettes for transgenics and gene targeting |
Centrifuges 5424, 5425 and 5425R | Eppendorf | Transgenics and gene targeting reagents preparation or genotyping use |
Mastercycler gradient | Transgenics and gene targeting reagents preparation or PCR for genotyping | |
Micro Forge MF-900 | Narishige International USA | Prepare microinjection holding or injection glass pipettes for transgenics and gene targeting |
Narishige Pneumatic Microinjector | Narishige International USA | Intracytoplasmic sperm injection |
ECM 830 Electro Square Porator | BTX Harvard Apparatus | Transgenics and gene targeting mouse embryo electroporation |
Electroporation System | ThermoFisher Scientific Neon Transfection System | Nucleic acid and protein electroporation |
Transgenics and Gene Targeting - CRISPR/Cas9 Genotyping | Service | Service to genotype founders and G1 progeny from a CRISPR project |
Transgenics and Gene Targeting - CRISPR/Cas9 Germline Transmission | Service | Service to subclone and sequence a PCR amplicon from founders of a CRISPR project |
Transgenics and Gene Targeting - Germline Transmission | Service | Service to breed chimera from mouse ES cell injection or founders from a CRISPR project with WT mice for germline transmission |
Transgenics and Gene Targeting - Sperm Cryopreservation | Service | Service to cryopreserve sperm from males provided by investigators |
Biospecimen Banking - Freezer | SP Scientific, Bio-cool IV40A | Embryo cryopreservation at a controlled slow rate |
Microinjection system | Prime Tech, Piezo Drive PMM4G | Intracytoplasmic sperm injection |
Contact: Suzanne de la Monte
Location: 55 Claverick Street »See on the MapAffiliated Centers: Liver Research Center, Center for International Health Research, Center for International Health Research, Liver Research Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Behavioral Phenotyping - Four Lane Rotarod | Emitter-Columbus Rotamex 5 Controller | Used to assess motor coordination and balance |
Behavioral Phenotyping - Morris Water Maze | Fabricated in house | Opaque white pool with platform for behavioral swim test. |
Biospecimen Banking - Freezer | HHP Barcoding Instrument | Barcode samples for identification. File Pro software. |
Centrifuge | Beckman Coulter Allegra 64R Refrigerated Centrifuge | For use with 50 mL conical tubes. |
Cryostat | Leica Cryostat | Frozen Tissue Sectioning. UVC disinfection and AgProtect minimizes contamination risk. Optional section waste and vacuum, disposable blade holder. |
Disrupter | Siegen Tissue Lyser | Disrupts multiple biological samples through high speed shaking in plastic tubes with steel, tungsten carbide, or glass beads. Up to 48 samples can be processed simultaneously. |
Imaging System | Applied Scientific Instrumentation MS-1000 | XY stage designed to provide a high resolution, and highly repeatable, means of controlling the X and Y position of a microscope stage |
Imaging System - Camera | Olympus U-CMAD3 camera | Connected to Olympus Inverted CKX31 Fluorescent Microscope. Digital Imaging of samples. 1.3, 3.0, 5.0 megapixel available. Vivid colors, 12-bit image capture, high-speed readout, external trigger output, USB 2.0 interface for universal connections. |
Imaging System - Camera | Sony 3CCD Exwave HAD Digital Camera | Connected to Olympus BX60. Digital Imaging of samples. 1.3, 3.0, 5.0 megapixel available. Vivid colors, 12-bit image capture, high-speed readout, external trigger output, USB 2.0 interface for universal connections. |
Imaging System - Camera | Stereologer 200 | 1/3" type DSP 3-CCD PAL color video camera with improved camera sensitivity (F8 at 2000 lx). Resolution of 800 TVL and a high S/N ratio of 61 dB. |
Microplate Reader | Molecular Dynamics M5 Spectracount | Cross talk (96-well): 3X 10-5, sensitivity: <20 attomol ATP per well, <0.2 firely luciferase per well, spectral range: 380-630 nm. |
Microplate Reader | Packard-Perkin Elmer Topcount | Analysis of cell proliferation, receptor binding assays, solid phase RIA/LIA, reporter gene assays, 51Cr release assays, HPLC fraction analysis, scintillation proximity assays, etc. |
Microplate Reader | Molecular Devices UV Max Microplate Reader | 96-wellplate reader. Spectral range: 405-630 nm. |
Microscope | Olympus BX60 | High-resolution digital pictures using conventional manual filter cubes or more versatile automated filter wheels, and can superimpose multi-color pictures |
Microscope | Olympus Inverted CKX31 Fluorescent Microscope | Inverted microscope with the capability of a fluorescence illuminator for GFP; documentation of images with digital imaging and video monitoring. Use for petri dishes, culture flasks, and multi-well plates. |
Microscope | Olympus Inverted Fluorescent microscope | FN22. 9mm stroke, coaxial coarse and fine focusing knobs. Long working distance universal condenser. |
PCR Thermal Cycler | Roche Lightcycler 4810 | Exchangeable 96 or 384 multiwell plate format. Ideal for multiplexing and automated workflows. Provides various methods for gene detection, gene expression analysis, genetic variation analysis and array data validation. |
Robotic Workstation - DNA Extraction | QUIAGEN Biorobot EZ1 | Fully automated purification of genomic DNA from human whole blood and viral nucleic acids from serum or plasma samples. |
Robotic Workstation - Pipetting | Eppendorf EpMotion 5070 | Automated pipetting. Four deck positions can hold tips, reagent reservoirs, single tubes, tube racks, micro-plates, or PCR plates with up to 384 wells. Suited for serial dilutions, reagent distribution, transfer from tubes to plates and normalization. |
Robotic Workstation - Pipetting | Tecan Genesis 200 Robot | Automated robot for pipetting tasks. Two arms- the LiHa (liquid handler) and the RoMa (robot manipulator). LiHa- conductivity-sensing 8-channel pipetting arm dispenses volumes of 5-1000 μL; RoMa- manipulating arm picks up and moves objects on the workstation. |
Spectrophotometer | Thermo Scientific NanoDrop 2000 | Measures DNA, RNA, and protein concentrations, and purity (A260/A280 ratio). Conc. range= 2 ng/microliter-15,000 ng/microliter; Min Sample size = 0.5 microliter. |
Suspension Array System | Luminex Magpix | Reads magnetic bead-based immunoassays. Automated reading of 96-well plates (~60 min/plate). Multiplex analysis of up to 50 biomolecules in a single sample. |
Suspension Array System | Bio-Rad BioPlex 100 | Automated reading of 96-well plates, up to 9,600 data points in about 35 min. Multiplex analysis of up to 100 biomolecules in a single sample via suspension array. |
Suspension Array System | Bio-Rad Vacuum manifold | Vacuum purification platform for single 96-well plates or individual luer-ended spin columns. Compatible with Bio-Plex 200 suspension array system. |
Contact: Michael Jibitsky
Location: Room 010, Engineering Research Center Building »See on the MapAffiliated Centers: Instrumentation for Molecular and Nanoscale Innovation (IMNI)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Deposition System - Thin Film Deposition | Angstrom - Multi-Target Sputtering System | This system accommodates four 3-inch sputtering sources and a variety of PVD processes (RF and DC). The temperature of the substrate can be increased up to 800˚C. |
Chemical Hood | Wet chemistry workbenches equipped with fume hoods for lithography | The Microelectronics Facility has a number of wet chemistry workbenches equipped with fume hoods for chemical processing (cleaning, wet etching, electroplating, anodization, etc) employed in the fabrication of semiconductor and nanomaterials devices. These hoods also house spinners for applying photoresist for lithography and other polymers. |
Lithography - Mask Aligner | Karl Suss MJB3 UV300 Mask Aligner for Lithography | The Karl Suss MJB3 UV300 3-inch mask aligner is designed for high-resolution photolithography, with a 350 W mercury lamp and Suss diffraction-reducing exposure optics. The primary exposure wavelengths of 365 or 403 nm lead to roughly 1 µm minimum feature size. For smaller feature sizes, electron-beam lithography is available in the Electron Microscope Facility. |
Lithography - Maskless Aligner | Heidleberg MLA 150 Maskless Aligner | MLA150 Maskless Aligner is a high-speed, direct-write, optical lithography system to fabricate nano- and micro-patterned materials and surfaces at low-cost and over large areas. The instrument comprises two laser illumination modules, optical systems to steer the laser beams and control exposure, integrated cameras for high-accuracy alignment to pre-existing structures, automated translation stage, real-time pneumatic autofocus, and a class 10 climate controlled chamber to achieve photo-lithographically defined structures with feature size ≤1μm. |
Lithography - Flexible Mylar-Mask | Newport-Oriel flexible Mylar-Mask Lithography System | Newport-Oriel flexible Mylar-Mask Lithography System is designed for printing from flexible Mylar masks onto substrates as large as 4” x 4” square or 5” diameter. It is capable of printing 5-micron features with placement and alignment to approximately 2 microns with appropriate alignment marks. |
Evaporator - Thin Film Deposition | Electron Beam Evaporator | This system deposits thin films of inorganic materials, usually metals, from a four-pocket electron-beam source inside a high vacuum (cryopumped) chamber. Deposition rates of up to 0.1 micron/minute are possible for some materials. This system can easily accomodate relatively large, 3-D objects and has substrate heating to 300 C for flat substrates. Restrictions on what materials may be evaporated are somewhat less stringent than for our other evaporators. |
Lithography - Imprinter | NIL Technology Nano Imprinter | The Nano Imprinter system offers thermal and UV nanoimprint and imprinting in vacuum if needed. The system transfers the patterns, usually nanohole or nanopillar arrays, from a silicon stamp to a nanoimprint-polymer-coated substrate by applying heat and pressure. |
Evaporator - Thin Film Deposition | Angstrom Organic Evaporator | The Angstrom Resitive-heated Evaporator is a thermal evaporator coupled with a nitrogen glove box. It is for depositing thin organic and inorganic films when either the deposition material or the substrate is adversely affected by moisture or oxygen. Samples and/or evaporants are loaded from a nitrogen glove box maintained at under 10 ppm of oxygen and under 1 ppm of water vapor. |
Deposition System - Thin Film Deposition | Cambridge NanoTech Atomic Layer Deposition System | The Cambridge NanoTech Fiji F200 series Atomic Layer Deposition System is a modular medium to high-vacuum ALD system that accommodates a wide range of deposition modes. This tool is currently configured with precursors for metallic and oxide layers of Hafnium, Aluminum, and Tungsten. |
Deposition System - Thin Film Deposition | Plasmatherm Model 790, Fluorine Chemistry Reactive Ion Etching Plasma Enhanced Chemical Vapor Deposition System (RIE PECVD) | The PlasmaTherm Model 790 RIE-PECVD system has a computer-controlled single-wafer turbopumped chamber that provides reactive ion etching (RIE) and plasma-enhanced chemical vapor deposition (PECVD) capabilities. RIE is accomplished using fluorine-chemistry gases (CF4, CBrF3, CHF3, SF6, etc.) with up to 500 W RF power. PECVD capability provides low-temperature (up to 350 C) SiO2 and Si3N4 deposition from silane chemistry. |
Profilometer - Characterization | DekTak3 Profilometer | The DekTak3 is a computer-controlled surface profile measuring system, which accurately measures step heights from below 50 A to over 50 µm by moving a diamond-tipped stylus over the surface. Equipped with video camera and surface profile analysis software. |
Analytical Prober | Karl Suss 200mm Precision Manual Analytical Prober | 200mm Precision Manual Analytical Prober. The PM8 provides the stability and resolution required for precise probe positioning. Manual submicron semiconductor failure analysis and in-process testing probe station. Submicron precision and stability with a fine-glide chuck stage and 8 in. x 8 in. X-Y movement. As fast as a motorized stage but with the simplicity and resolution of a manual system. Vacuum Chuck: 8 in. dia. Mitutoyo high magnification microscope with two long working distance objectives. Microscope lifts for easier access to the probes and device under test. The station is fitted with six Wentworth micromanipulators having tungsten needle probes with 3 micron tips. |
Deposition System - Thin Film Deposition | Lesker Lab 18 thin film deposition system | The Lab-18 thin film deposition system is a combined electron-beam evaporator and RF sputtering Physical Vapor Deposition (PVD) system that makes high quality, research-grade metallic, semiconducting, and dielectric thin films. The Lab 18 includes a four-pocket electron beam evaporator as well as two 2” magnetron sputter sources. Two mass flow regulated gas lines provide for control of chamber pressure and gas composition for reactive sputtering. |
Plasma Etching System | Inductively Coupled Plasma RIE System | The SPTS LPX is an inductively-coupled plasma (ICP) reactive-ion etcher (RIE). It can etch high-aspect ratio structures in a wide range of materials, including silicon, silicon dioxide, and silicon nitride. This system is configured with a range of gases for reactive- and physical-ion etching, including SF6, C4F8, CF4, O2, and Ar. The LPX-ICP system is equipped with a single-wafer load-lock transfer arm for 100mm wafers; smaller wafers and small pieces may be processed on the system using a carrier wafer. |
Characterization - Thin Film Deposition | JA Woollam, M-2000 Ellipsometer Variable Angle Research Grade | The JA Woollam, M-2000 Variable Angle Research Grade ellipsometer measures angle- and wavelength-dependent reflectivity and transmissivity data over a wide spectral range (from ultraviolet to near-infrared) that is analyzed to directly to determine optical properties of single- and multi-layer films (e.g., their thickness and complex wavelength-dependent refractive index), which provide insight into their underlying chemical, electronic, and mechanical properties. |
Furnace | Wet and Dry Oxididation | Quartz tube furnace for thermal oxide growth on silicon by a chemical reaction between the silicon and either dry oxygen or water vapor at atmospheric pressure. The typical temperature range for the oxidation of silicon for wafer fabrication 750˚C to 1100˚C. The tubes accommodate multiple 2 inch wafers. |
Contact: Hector Garces
Location: Institute for Molecular & Nanoscale Innovation (IMNI), Room 104, Prince Lab »See on the MapAffiliated Centers: Instrumentation for Molecular and Nanoscale Innovation (IMNI)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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X-Ray Diffraction System, 2 Dimensional | Bruker, D8, XPS | This Bruker D8 Discover X-ray Diffraction System can be configured with a point and line source x-rays. A Vantec 500 2D area detector or a LinxEye (0D, 1D)detector can be used for the analysis. The system is suitable for texture, stress, and micro-x-ray diffraction as well as for orientation and epitaxy studies. The instrument has in-situ capabilities for studies at low/high temperature and under vacuum to study properties as a function of time, temperature, gas environment or vacuum. A capillary spinner stage is also available for investigations of air sensible materials with the sample prepared in a capillary glass. |
X-Ray Diffration System, High-Resolution | Bruker, D8, XPS | This Bruker D8 Discover X-ray Diffraction System includes a line source configuration system with a LinxEye (0D, 1D) detector and a Scintillator detector. The system is suitable to determine thickness, crystallographic structure and strain in epitaxially thin films as well as high resolution analysis of bulk materials. X-ray reflectometry (XRR), high resolution XRD, grazing incidence diffraction (GI-XRD), in-plane GID (IGID) and reciprocal space mapping (RSM) are some techniques that can be performed with this configuration. High resolution is obtained using parallel beam optics: Goebel mirror, monochromators, soller slits and analyzer crystals. |
Microscope - Atomic Force | Asylum, MFP-3D, AFM | The Asylum MFP-3D Origin atomic force microscope (AFM) is a multi-mode instrument. It is equipped with a closed loop operation in all three axes with high precision, resolution and low noise operation. The closed loop Z scanner eliminates hysteresis. The AFM provides a 90 µm X and Y range (< 0.5nm noise) and 15 µm Z range (Z sensor, 0.25 nm noise) and detector noise <15 pm. It also includes Inverted optical lever design that eliminates interference artifacts and a low-coherence infrared (860 nm) super-luminescent diode (SLD) light source (class 1M). Applications include dual AC resonance tracking (DART), electric force microscopy (EFM), force mapping mode, lateral force microscopy (LFM), magnetic force microscopy (MFM), kelvin probe force microscopy (KPFM), piezo-response force microscopy (PFM), and conductive AFM (C-AFM) with ORCA. |
Microscope - Confocal Raman | Witec, Alpha 300 M+, CRM | The Witec Alpha 300 Confocal Raman Microscope is equipt with different scan modes for local Raman spectra, mapping distribution of phases within a sample and with 3D image generation and depth profile of samples. The depth profiling and 3D imaging capabilities of the Alpha 300 provide the ability to analyze the interior of transparent samples without microtome sectioning or freeze etching. Multi-wavelength coupler for selection of 532 nm or 633 nm wavelength laser sources and a Ray Shield coupler for measurements of Raman spectra at low wave numbers. Fast Raman imaging with integration time in the milliseconds order per spectrum. A heating stage for in-situ measurements in the temperature range 25°C to 1400°C with a thermal stability of ± 0.3°C. |
Spectrometer - Electron Paramagnetic Resonance (EPR) | Bruker, EMX+, EPR | The Bruker EMX Electron Paramagnetic Resonance (EPR) Spectrometer is equipt for the Technique for the direct detection of paramagnetic species. The EMX EPR has a digital resolution of 24 bit center field, a field sweep up to 256,000 points, high-sensitivity resonator with irradiation window, dual channel, simultaneous detection of 1st and 2nd harmonic, and sensitivity S/N up to 2000:1. The system has a helium temperature control system for low temperature measurements in the temperature range 3.8 K (~-269 °C) to 300 K (~27°C). Applications span a wide range of areas an include kinetics of radical reactions, oxidation and reduction process, polymerization reactions, degradation of compounds by light, organometallic compounds and catalysis, free radicals, and antioxidants and radical scavengers. |
Contact: Kristin Fortin-Ashburne
Location: Butler Hospital »See on the MapAffiliated Centers: Carney Institute for Brain Science, IDeA COBRE Center for Neuromodulation (CCN) at Butler Hospital
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Neuromodulation and Neuroimaging Research Services Contact: Kristin Fortin-Ashburne NNC Core | The Neuromodulation and Neuroimaging Core (NNC) facilitates the research goals of COBRE Project Leaders and pilot grant investigators and benefits the broader neuromodulation research community by providing expert support, training, assistance, and advice to its constituents in the practical aspects of implementation, data collection, and project management related to neuroimaging and neurostimulation methods. | The NNC addresses a critical need, namely, to provide Project Leaders with unique resources, skills, and support for using the best methods and practices surrounding neuroimaging and neuromodulation in clinical neuropsychiatric populations. The NNC provides key services for research projects and creates an enduring resource that offers neuromodulation researchers a series of educational seminars, hands-on workshops, and customized training opportunities, high-level consultative and medical input, and use of a specialty research facility at Butler Hospital with lab space, equipment, and medical support. |
Contact: Russell Hopson, PhD
Location: MacMillan Hall »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker 600 Avance III HD Ascend | z-BBFO probe for all nuclei (most sensitive for all nuclei) |
Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker 400 Avance III Ultra-Shield | z-BBFO probe (2nd most sensitive for 1H and broadband nuclei) - (HR-MAS probe) |
Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker 400 Avance Ultra-Shield | z-BBO (3rd best sensitivity for broadband nuclei) |
Contact: Xiaobing Tian, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Assist investigators with experimental plans for drug synthesis for lead optimization. Facilitate organic synthesis efforts through interactions with the Department of Chemistry, Industry or other collaborations. Provide support for grant submissions. |
Contact: Lanlan Zhou, MD, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Advise and assist investigators with the production of organoids to facilitate cancer biology, drug efficacy and toxicity evaluation of therapeutics towards tumor versus normal organoids that express different cancer gene mutants. Provide support for the growth and maintenance of organoid and spheroid cultures including passaging. Maintain a collection of established organoids that can be provided to investigators for research. Develop gene knockout and overexpression matched patient-derived organoids. Assist investigators with planning of drug screens working with the drug discovery capability. Provide support for grant submissions. |
Contact: Patrick Ewanchuk
Location: Albertus Magnus Hall »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM), IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Environmental Chamber Contact: Maia Bailey | Percival Scientific | 1 unit |
Environmental Chamber Contact: Maia Bailey | Percival Scientific, AR-22L Growth Chamber | For Arabidopsis |
Imaging System - Camera Contact: Pat Ewanchuk | Fastec HiSpec1, Low-Light High-Speed Camera | Innovative Low-Light High-Speed Camera. Monochrome with 2GB Memory, C-Mount, includes ImageBLITZ Auto Trigger, Burst Trigger Mode, Multi- Sequence Record Mode (1280 x 1024 at 506fps) 2 Units |
Microscope - Electron Contact: Joe DeGiorgis | Philips EM-300 Transmission Electron Microscope (TEM) | Transmission electron microscopy |
Sample Preparation - Laboratory - Prep Lab Contact: Joe DeGiorgis | Electron Microscopy Prep Lab | Laboratory space for preparing samples for electron microscopy |
Microscope - Electron Contact: Joe DeGiorgis | Amray-1000 Scanning Electron Microscope (SEM) | Scanning Electron Microscope equipped with EDEX for elemental analysis and a digital imaging system, a Zeiss Axiophot equipped for bright field, dark field, DIC and fluorescence microscopy with a digital imaging system |
Microtome Contact: Joe DeGiorgis | Porter-Blum MT-2B Ultramicrotome | Porter-Blum MT-2B UltraMicrotomes, 2 Units |
Aquatic System Contact: Pat Ewanchuk | Aquatic Habitats Glass Tank System and Life Support Controller | Glass tank system and life support controller with low water level protection (18-10 gallon tanks) |
Imaging System - Visualization- Camera lucida Contact: James Waters | Leica Drawing tube | Drawing tube, for MZ and M-series, the object and the drawing surface are visible at the same time, suitable for drawing for left and right handed persons, work is possible in the daylight. Documents and scales can be mirrored-in and photographed along with the object. |
Analyzer - Respiratory physiology Contact: James Waters | LI-COR Biosciences - Li-7000 CO2/H2O | The LI-7000 is a high performance, dual cell, differential gas analyzer. It uses a dichroic beam splitter and two separate detectors to measure infrared absorption by CO2 and H2O in the same gas stream. The LI-7000 is ideally suited for applications that demand high speed, high precision measurements in absolute and/or differential modes, including plant and insect gas exchange using chamber-based methods, atmospheric-surface flux eddy covariance and Bowen ratio techniques, vertical profiling, and general atmospheric monitoring. |
Contact: Patrick Ewanchuk
Location: Hickey Hall »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM), IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
DNA Sequencer Contact: Elisabeth Arevalo | ABI Prism 3100 Genetic Analyzer | Used for DNA sequencing and fragment analysis. The ABI PRISM 3100 Genetic Analyzer is an automated capillary electrophoresis system that can separate, detect, and analyze up to 16 capillaries of fluorescently labeled DNA fragments in one run. |
PCR Thermal Cycler Contact: Charles Toth | Stratagene Mx3000p Real-time PCR Thermal Cycler | This instrument is available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies. Agilent's qPCR software, MxPro, provides users with an intuitive interface, quick experiment design, powerful data analysis and easy report generation. |
Fluorescence Activated Cell Sorter (FACS) Contact: Charles Toth | Flow Cytometer- Accuri C6 | The BD Accuri C6 is a personal flow cytometer that is easy to use, simple to maintain, and affordable. |
Cold Room Contact: Charles Toth | Walk-In Cold Room | |
Analyzer - Cell Metabolism Contact: Marla Tipping | Seahorse Biosciencies, XFe Extracellular Flux Analyzers, XFe96 | Platform for metabolic assays that simultaneously measures the two major energy producing pathways of the cell - mitochondrial respiration and glycolysis - in a microplate, in real-time. |
Laboratory - Tissue Culture Contact: Charles Toth | Tissue Culture Laboratory | Space and instrumentation for tissue culture preparation |
Imaging System - Gel Imager Contact: Yinsheng Wan | LI-COR Odyssey IR (Infrared) Imaging System | Instrumentation for quantitating western blots. |
Fluorescence Activated Cell Sorter (FACS) Contact: Nicanor Austriaco | Cellometer Vision Image Cytometry | The Vision CBA System combines the simplicity of image cytometry with the power of flow analysis software to offer user-friendly cell-based assays |
Spectrophotometer Contact: Charles Toth | Thermo Scientific NanoDrop | UV/VIS |
Contact: Patrick Ewanchuk
Location: Sowa Hal »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM), IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Microscope Contact: Joe DeGiorgis | Zeiss LMS 700 Flexible Confocal Microscope | Performs fluorescence measurements, creates optical sections of samples and combines them into 3D image stacks. |
Contact: Catherine Curtin-Miller
Location: URI, Avedisian Hall »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
current Good Manufacturing Practice (cGMP) Facility | Service | Rapid expansion in the markets for biologics and small molecule pharmaceutical products has created a critical demand for skilled professionals (from equipment operators to advanced technical and quality assurance personnel) and additional infrastructure to support drug development and manufacturing. The current Good Manufacturing Practice (cGMP) Facility at the University of Rhode Island (URI) offers a state-of-the-art training, development and manufacturing facility for GMP production of solid oral dosage forms. |
GMP manufacturing | Clinical Research Service, GMP manufacturing | Assisting academic, government and industry partners with the manufacture of solid dose forms for veterinary and human use |
GMP analytical testing | Clinical Research Service, GMP analytical | Providing pharmaceutical testing services including turn-key stability studies |
Training Center | Clinical Research Service, GMP education | Providing GMP industrial experience in drug manufacturing and analytical testing for students in various related undergraduate, graduate and adult education programs, as well as industry-specific training in collaboration with pharmaceutical and biotechnology firms. |
Contact: Nicholas Vasques
Location: Building for Environmental Research and Teaching »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Greenhouse Contact: Nicholas Vasques | Plant environmental center with seven separate, computer controlled rooms where conditions like lighting, temperature, and humidity can be fine tuned; an additional 17 controlled plant growth environments provided by growth chambers (15) and walk-in growth rooms (2) | A plant environmental center with a suite of supporting facilities, including a classroom, a headhouse for potting and other prep work, and growth chambers for plants. |
Affiliated Centers: ECHO-NOVI: Environmental Influences on Child Health Outcomes
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Program for Environmental influences on Child Health Outcomes (ECHO) | Clinical Research Resources, Environmental Influences on Child Health | The ECHO program investigates how exposure to a range of environmental factors in early development — from conception through early childhood — influences the health of children and adolescents. |
Contact: Paul Bertone, PhD
Location: The Coro West Building »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
High Performance Liquid Chromatography (HPLC) | Agilent 1290 Infinity II | Modular UHPLC system with automated multisampler and quaternary pump for analyte separations up to 600 bar. |
Bioinformatics Analysis - MASCOT | Software: Matrix Science MASCOT Search Engine | Software for MS data analysis through statistical evaluation of matches between observed and projected peptide fragments. |
Bioinformatics Analysis - ProteoIQ | Software: NuSep ProteoIQ Mass Spec Data Analysis | Software for post-analysis of MASCOT results. Protein and peptide probabilities generated and protein relative quantitation is performed by spectral counting. Normalized and with standard deviations. |
Imaging System - Gel Imager | Bio-Rad Molecular Imager VersaDoc | Quantitative digital imaging system for single or multi-color images. Includes multiplexed samples (1-D or 2-D gels), chemiluminescent and chemifluorescent blots, and microplates. |
High Performance Liquid Chromatography (HPLC) | Thermo Scientific nLC 1200 | High-performance LC for nanoflow resolution of complex analyte mixtures at up to 1200 bar. |
Liquid Chromatography - Mass Spectrometer (LC - MS) | Thermo Scientific Q Exactive Plus Mass Spectrometer | LC-MS/MS system combines high-performance quadrupole with high resolution, accurate-mass (HR/AM) Orbitrap for total proteome characterization. Max resolving power= 140,000; max scan speed= 12Hz, Intra-scan dynamic range > 5000:, Quadrupole mass filter. |
Spectrophotometer | Thermo Scientific NanoDrop 2000 | Measures DNA, RNA, and protein concentrations, and purity (A260/A280 ratio). Conc. range= 2 ng/microliter-15,000 ng/microliter; Min Sample size = 0.5 microliter. |
Technical Assistance - Mass Spectrometry | Service | Service to provide Imaging Mass Spectrometry Data Analysis. |
Technical Assistance - Protein Determination | Service | Service to provide Protein Determination (BCA assay). |
Technical Assistance - Protein Extraction | Service | Service to provide ReadyPrep Protein Extraction Kit (Total Protein and Sequential Extraction). |
Technical Assistance - Protein In-Gel Digestion | Service | Service to provide In-gel digestion LC/MS analysis and protein database search. |
Technical Assistance - Protein In-Solution Digestion | Service | Service to provide In-solution digestion LC/MS analysis and protein database search. |
Technical Assistance - Protein Quantification | Service | Service to provide Data interpretation and protein quantification. |
Technical Assistance - Protein Sample Clean-up | Service | Service to provide ReadyPrep 2D Cleanup Kit. |
Mass Spectrometer | Thermo Scientific TSQ Quantiva | Triple-quadrupole MS for identification and absolute quantification of biomarkers, target proteins/peptides with known post-translational modifications in selected mass ranges. |
Microplate Reader | GloMax Discover - Multimode Detection System | Multi-mode detection instrument offering flexible use of filters and includes: high-performance, luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities |
Cell Counter | Thermo Scientific Countess II Automated Cell Counter | Automated image analysis of cell suspensions providing rapid measurement of total cell counts, viability and average cell size. |
Contact: Nicholas DaSilva, PhD
Location: 3rd Floor, Laboratories for Molecular Medicine »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Analyzer - Calorimeter | Microcal Isothermal Titration 200 (ITC) | Characterization of molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules. |
Analyzer - Calorimeter | Microcal VP Differential Scanning (DSC) | Measurement of heat changes that occur in biomolecules during controlled increase or decrease in temperature. |
Fluorometer - Spectrofluorometer | Horiba Jobin Yvon FluoroMax-4 | Fluorescence detection. A heated cell allows temperature studies. |
Spectrometer | JASCO J-815 Circular Dichroism (CD) Spectrometer | Protein folding studies, Protein conformational studies, DNA/RNA interactions, Enzyme kinetics, Purity testing of optically active substances, quantitative analysis of pharmaceuticals, natural products chemistry, biochemistry and macromolecules, rapid scanning (time resolved) experiments. Peltier device for temperature studies. |
Spectrophotometer | Thermo Scientific NanoDrop 8000 | A full spectral microvolume (microliter) UV-Vis analysis spectrophotometer for measuring DNA, RNA, and protein of 8 samples simultaneously. |
Technical Assistance | Service | Service to provide Proteomics Technal Assistance |
Mass Spectrometer | Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer | Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer is a bench-top ESI LC-MS/MS system that combines quadrupole precursor ion selection with high-resolution, accurate-mass (HRAM) Orbitrap detection. |
Contact: Matthew Cabral, PhD
Location: Fascitelli Center for Advanced Engineering »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Sample Preparation - Sputter Coater System Contact: Irene Andreu | Cressington 7002-8 sputter coater with film thickness monitor | Coat non-conducting samples with gold for use on the SEM, rotating/tilting stage for coating of large specimens. |
X-Ray Diffraction System Contact: Irene Andreu Request a Service | Rigaku Ultima IV multipurpose X-ray diffraction (XRD) system | micro-crystalline diffraction, thin-film diffraction, small angle scattering, and in-plane scattering. |
Microscope - Field Emission-Scanning Electron Microscope (FE-SEM) Contact: Irene Andreu SIGMA VP FE-SEM Request a Service | Zeiss Gemini Sigma VP Field Emission-Scanning Electron Microscope (FE-SEM) | Field emission scanning electron microscope Zeiss Gemini Sigma VP, with cryoSEM (Gatan), energy dispersive X-ray spectroscopy (Oxford Instruments), variable pressure mode, backscattering detector, in lens detector and secondary electron detector |
Microscope - High Content Screening System | Perkin Elmer - Opera Phenix | High Content Screening System: Automated confocal fluorescence microscope designed for high-throughput 3D imaging of fluorescent samples in microplates, live-cell imaging, drug screening, spheroid imaging |
Sample preparation - Plunge freezer Contact: Irene Andreu Vitrobot Mark IV | FEI Vitrobot Mark 2 Plunge Freezer for cryoTEM | Automatic plunge freezer for cryoTEM sample preparation |
Microscope - Confocal Raman Spectroscopy Contact: Irene Andreu | WITec alpha 300R | Confocal imaging and chemical analysis by Raman spectroscopy up to 100x magnification, 3D chemical imaging, large area imaging, principal component analysis of chemical composition, upright and inverted mode, automated XY stage, 785 and 532 nm excitation lasers |
Microscope - Fourier Transform Infrared Spectroscopy | Shimadzu AIM-9000 with Shimadzu IRTracer100 | FTIR microscope and spectrometer, ATR, liquid and gas cell. |
Sample Preparation - Automated pipettor | Opentrons OT-2 | Automated sample preparation platform for staining well plates |
Microscope - X-ray Contact: Irene Andreu | Zeiss Xradia Versa 610 with in situ tensile, compression and temperature stage | X-ray microscopy and tomography of samples up to 10 cm in diameter with resolution up to 500 nm |
Spectrometer - Energy Dispersive X-Ray Fluorescence Contact: Irene Andreu | Shimadzu EDX-8100 | Energy dispersive X-ray fluorescence of powders, solids and liquids. Ambient pressure, vacuum and He atmosphere |
Microscope - Scanning Transmission Electron Contact: Irene Andreu | JEOL F200 | Scanning transmission electron microscope with cryogenic capabilities, direct electron camera (Gatan K3), Energy dispersive X-ray spectroscopy (JEOL), electron energy loss spectroscopy (Gatan). Sub nanometer resolution, 80 and 200 kV operation, TEM, STEM and diffraction |
Microscope - Atomic Force Contact: Irene Andreu | Shimadzu SPM-9700 | Scanning probe microscope for atomic force microscopy, magnetic force microscopy, liquid cell analysis, Kelvin force microscopy, I/V curves, nanomechanical measurements |
Contact: Dr. Ang Cai
Location: Avedisian Hall »See on the MapAffiliated Centers: IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker Avance III 300 with BBO probe | Optimized for small molecule NMR |
Analyzer - Protein Quality | Tycho NT.6 | The Tycho NT.6 verifies protein quality by looking at the structural integrity (or foldedness) of a protein. Changes in the intrinsic fluorescence of the sample are measured as a thermal ramp is applied. Changes in the fluorescence signal indicate transitions in the folding state of the protein. The Tycho can be used to study the stability of protein samples in different buffers or at different pH values. The measurement is completed in three minutes and uses a capillary tube to hold the sample. |
Characterization - Surface Plasmon Resonance | Biacore T200 SPR | The Biacore T200 is a surface plasmon resonance instrument which can be used to characterize molecular interactions between biomolecules ranging in size from ions to viruses. The Biacore software provides a range of tools for kinetic analyses. These analyses can be performed using a multicycle approach (many samples against one ligand or when different ligands are immobilized), or alternatively, using fast, single cycle kinetics. |
Analyzer - Live Cell | Roche xCELLigence Real-Time Cell Analysis System | Label free live cell analyzer |
Biological Safety Cabinet | NuAire, Laminar Flow Hood | Cell culture facility |
Cell Counter | Nexcellom Cellometer Vision Cell Counter | Automated cell counter |
Centrifuge | Beckman Avante High Speed Centrifuge | High speed centrifuge |
Centrifuge | Thermo MultiFuge Centrifuge table-top | Centrifuge |
Centrifuge - Ultra | Beckman Coulter Optima Beckman Optima L-100 XP ultracentrifuge | Ultra centrifuge |
Centrifuge - Ultra | Sorvall Micro Ultra Centrifuge | Ultra centrifuge |
Fluorescence Activated Cell Sorter (FACS) | Becton Dickinson (BD) Biosciences FACS Verse Flow cytometer | Fluorescence-activated cell sorting |
Electrophoresis System - Gel Electrophoresis | Bio-Rad 2D Gel System | Two Dimensional (2D) Gel Electrophoresis |
Liquid Chromatography | GE AKTA Fast Protein Liquid Chromatograph (FPLC) | FPLC |
Chromatography | Teledyne-ISCO CombiFlash System | Flash Chromatography |
Imaging System - Gel Imager | LI-COR Odyssey IR (Infrared) Imaging System | IR gel imager |
Liquid Chromatography - Mass Spectrometer (LC - MS) | AB SCIEX TripleTOF 4600 and Shimadzu Prominence UFLC sytem | ESI-TOF MS |
Mass Spectrometer | Shimadzu Axima MALDI-TOF MS/MS | MALDI MS |
Mass Spectrometer | Thermo iCAPQ-a ICP-MS | Elemental Analyzer |
Microplate Reader | Molecular Devices SpectraMax M2 | Fluorescence/UV/VIS |
Microplate Reader - Luminometer | Promega GlowMax System | Chemiluminescence |
Microscope | Nikon Eclipse E600 Upright Microscope | Microscope |
Microscope | Nikon Eclipse Ti2 inverted confocal microscope | Confocal Microscope |
PCR Thermal Cycler | Applied Biosystems/Life Technologies ABI Vii7- qPCR | Real Time-PCR |
PCR Thermal Cycler | Eppendorf Mastercycler PCR | PCR |
Spectropolarimeter | Jasco J-1100 Circular Dichroism (CD) Spectropolarimeter | CD |
Spectrometer | Thermo Nicolet 380 Fourier Transformed Infrared Spectrometer (FT-IR) | FT-IR |
Chromatography | Metrohm Ion Chromatography 940 Vario | Ion Chromatography |
Spectrophotometer | Thermo Scientific NanoDrop | UV/VIS |
Water Purification System - Deionized (DI) | Millipore Milli-Q Water System | DI Water System |
Western Blotting System | Bio-Rad Turbo Trans Blot system | Western Blotting |
Synthesizer, DNA/RNA | MerMade-4 Oligonucleotide synthesizer | DNA/RNA Synthesizer |
High Performance Liquid Chromatography (HPLC) | Shimadzu Nexera-i HPLC system | High Performance Liquid Chromatography |
Microscope | EVOS FL Auto Cell Imaging System | Cell-based imaging applications—including live-cell imaging, image tiling, and Z-stacking |
Spectrometer | Jasco Digital Polarimeter P2000 | Polarimeter |
Imager - Biomolecular | Azure Sapphire Biomolecular Imager | The Azure Sapphire Biomolecular Imager is a laser scanning system has four lasers (488, 520, 658 and 784 nm) giving a wide range of excitation. The instrument supports long and short wavelength Near IR fluorescence, red/green/blue imaging, and chemiluminescent imaging. Multiple detectors are used depending on the excitation wavelength and application for maximum sensitivity. It can accommodate gels up to 25cm by 25 cm. |
Incubator | Heraeus Heracell Double Stacked Incubator | The Heraeus Heracell Double Stacked Incubator has Copper Chambers, which protects samples while optimizing cell growth with fast recovery characteristics. |
Nuclear Magnetic Resonance (NMR) Spectrometer | Varian 500 MHz NMR spectrometer | Optimized for small molecule NMR |
Mass Spectrometer | ABSciex Qtrap 4500 Triple Quadrapole LC/MS | Has Shimadzu HPLC system attached |
Microplate Reader | Molecular Devices ID3 Microplate Reader | Uv/vis, Chemilumenesence, fluorescence |
Spectrophotometer | Genesys 50 UV/VIS | UV/Vis |
Imager and Microplate Reader | BioTek Cytation 5 Imager | fluorescence imaging |
Analyzer - Metabolism Analysis | Agilent Seahorse XFe96 Analyzer | 96 well plate metabolism analysis |
Contact: Christopher Hemme
Location: College of Pharmacy »See on the MapAffiliated Centers: IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island, Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Bioinformatics Analysis Contact: Christopher Hemme Bioinformatics and Data Science at the MIC | Service - Bioinformatics | We provide bioinformatics consultation and services to the Rhode Island INBRE community and to the general scientific community of Rhode Island. In addition to traditional bioinformatic services, our Core is expanding to 3D/VR/AR visualization services in conjunction with the URI 3D Visualization Laboratory. We are currently developing 3D/VR visualization apps for a variety of platforms including smartphones, Vive/SteamVR, and others. We also have access to the 3D printer at the URI 3D Visualization Core for printing of marcomolecules at a reasonable price. |
Bioanalyzer Contact: Janet Atoyan | Agilent Bioanalyzer 2100 | Provides fast and automated separation, sizing and quantification of DNA, RNA and proteins by miniaturized Lab-on-a-Chip electrophoresis |
Disrupter Contact: Janet Atoyan | Covaris S220 High Performance Ultrasonicator | Automated acoustic disruption and homogenization of cells and tissue samples using focused ultrasonic energy at 500 KHz. Performs DNA Shearing |
DNA Sequencer Contact: Janet Atoyan | Illumina MiSeq DNA NextGen Sequencing | Performs clonal amplification, genomic DNA sequencing, and data analysis with base calling, alignment, variant calling, and reporting. |
DNA Sequencer Contact: Janet Atoyan | Applied Biosystems 3500xl Genetic Analyzer | DNA Sanger Sequencing & Fragment Analysis: Fluorescence-based instruments utilizing capillary electrophoresis for DNA sequencing and fragment analysis. |
Fluorometer Contact: Janet Atoyan | Invitrogen Qubit | Fluorometer that uses fluorescent dyes bound to specific molecules to quantify DNA, RNA and proteins |
Sample Preparation - Library Preparation System Contact: Janet Atoyan | IntegenX Apollo 324 System NextGen Sequencing (NGS) Library Preparation System | Automated liquid handling instrument dedicated to the preparation of sample libraries for Next Generation Sequencing |
PCR Thermal Cycler Contact: Janet Atoyan | Roche LC480 qPCR Systems | Quantitative PCR (qPCR) instrument. Used for highly accurate qualitative and quantitative detection of nucleic acids and gene expression. |
Contact: Dr. Cameron Goodwin
Location: URI Bay Campus »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Reactor, Research and Test Contact: Cameron Goodwin | 2 Mega-Watt, light water cooled, pool type reactor | Neutron and gamma irradiation and experimental set-up, as well as analysis |
Contact: Dr. Rebeka Rand-Cyr
Location: Fogarty Life Science »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM), IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Sample Preparation - Knifemaker Contact: Breea Govenar | LKB 7800 Knifemaker | Slide/Sample Preparation |
Microtome Contact: Breea Govenar | Leica RM2165 Motorized Rotary Microtome | Slide/Sample Preparation |
Microtome Contact: Breea Govenar | TBS Microtome plus Cryostat | Slide/Sample Preparation |
Microscope Contact: Breea Govenar | American Optical Series 10 Fluorescence Microstart | Microscopy course |
Microscope Contact: Breea Govenar | Nikon E600 Epifluorescence with DIC/Phase and digital camera | Research |
Microscope Contact: Breea Govenar | Olympus CKS41 Inverted Microscope | Tissue culture general use |
Microscope Contact: Breea Govenar | Olympus Compound Light Microscope | General use |
Microscope Contact: Breea Govenar | Olympus FluoView 1000 Laser Scanning Confocal Microscope | Research and Microscopy course |
Microscope Contact: Breea Govenar | Olympus Inverted Epifluorescence Microscope with digital camera | Tissue culture/Research |
Microscope Contact: Breea Govenar | Zeiss SteREO discovery V8 Microscope with Digital Camera | Research |
Aquatic System - Wet Lab / Aquarium room Contact: Breea Govenar | Aquaria and related equipment | Research |
Autoclave Contact: Breea Govenar | Autoclave | Microbiology/Research |
Biological Safety Cabinet Contact: Breea Govenar | Labconco class II Biological Safety Cabinet | Used for tissue culture |
Biological Safety Cabinet Contact: Breea Govenar | Sterile laminar flow hood | Microbiology prep |
Centrifuge Contact: Breea Govenar | Eppendorf 5810R Refrigerated Centrifuge | Used for tissue culture |
Centrifuge Contact: Breea Govenar | Sorvall Discovery M120SE Mini Ultracentrifuge | Research/Cell fractionation |
Centrifuge Contact: Breea Govenar | Sorvall Superspeed RC2 Centrifuge | General use |
Centrifuge Contact: Breea Govenar | Sorvall Superspeed RC5 Plus Centrifuge | Research |
Centrifuge Contact: Breea Govenar | Thermo IEC Centra CL3R Refrigerated Centrifuge | Genetics and Cell & Molec courses |
Cold Room Contact: Breea Govenar | Walk-In Cold Room | Large cold room for experimentation and storage |
Dishwasher Contact: Breea Govenar | Dishwasher | Microbiology/Research |
Disrupter Contact: Breea Govenar | French Pressure Cell Press | Research |
Disrupter Contact: Breea Govenar | Polytron Tissue Grinder | Slide/Sample Preparation |
Disrupter Contact: Breea Govenar | Sonicator | General use and research |
Disrupter Contact: Breea Govenar | Sonicator with cup | Research and graduate courses |
Electroporation System Contact: Breea Govenar | Bio-Rad Micropulser Electroporator | Research |
Sample Preparation - Freeze Dry System Contact: Breea Govenar | ATMO VAC 5009 freeze dryer | Slide/Sample Preparation |
Biospecimen Banking - Freezer Contact: Breea Govenar | Thermolyne Liquid Nitrogen cryogenic storage system | Cell storage |
Biospecimen Banking - Freezer Contact: Breea Govenar | Ultralow Freezer | Used for freezing biological samples, 6 units |
Imaging System - Gel Imager Contact: Breea Govenar | InGenius Syngene Gel Documentation System | Research |
Ice Machine Contact: Breea Govenar | Ice Machine | General use |
Imaging System - Dark Room Contact: Breea Govenar | Imaging System - Dark Room | Gel imaging and analysis |
Incubator Contact: Breea Govenar | Non CO2 Incubator | Used for tissue culture |
Incubator Contact: Breea Govenar | Thermo CO2 Incubators | Used for tissue culture, 2 units |
Incubator Contact: Breea Govenar | Refrigerated Incubator | Used for tissue culture |
Laboratory - Tissue Culture Contact: Breea Govenar | Tissue Culture Room | A room outfitted with tissue culture instrumentation |
Microplate Reader Contact: Breea Govenar | Lenovo Automated Plate Reader | Research |
Microplate Reader Contact: Breea Govenar | Thermo Labsystems Multiskan MMCC/340 plate reader | Research |
Sample Preparation - Oven Contact: Breea Govenar | Drying Oven | General use |
PCR Thermal Cycler Contact: Breea Govenar | Bio-Rad IQS Real time PCR thermocycler | Research |
PCR Thermal Cycler Contact: Breea Govenar | Eppendorf Gradient Mastercycler | PCR instrumentation, 2 units |
Incubator - Shaker Contact: Breea Govenar | Incubator/Shakers | Multiple units |
Spectrophotometer Contact: Breea Govenar | BioRad SmartSpec Plus | Research |
Spectrophotometer Contact: Breea Govenar | Eppendorf Biophotometer | Gectrophotometerseral use |
Spectrophotometer Contact: Breea Govenar | Spectronic 21 D UV/VIS | Research |
Spectrophotometer Contact: Breea Govenar | Therm Spectronic Genesys UV/VIS Scanning | Research, 6 units |
Spectrophotometer Contact: Breea Govenar | Thermo Scientific NanoDrop ND-1000 | UV/VIS, 2 units |
Water Purification System Contact: Breea Govenar | Barnstead Still Water Purifier | General use for teaching labs |
Water Purification System Contact: Breea Govenar | Millipore DirectQ (X2) | Tissue culture |
Water Purification System Contact: Breea Govenar | Millipore NanoPure Diamond | 2nd floor research use |
Imaging System - X-ray Film Contact: Breea Govenar | X-ray film processor | Blot imaging |
Contact: David Chandler
Location: RISD Auditorium »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Imaging System - Audio|Visual | Kodak zi 10 Video Camera (8 units) | Portable HD pocket camcorder, batteries, adaptor cables, cases, accessories |
Imaging System - Audio|Visual | H4N ZOOM Audio Recorder (5 Units) | High performance portable digital audio recorder, batteries, adaptor cables, cases, accessories |
Imaging System - Audio|Visual | ZOOM Q3 Video Camera (7 Units) | Portable palm camcorder, batteries, adaptor cables, cases, accessories |
Imaging System - Audio|Visual | EPS-SonyPMWEX1R-01 (2 Units) | High performance HD CMOS solid state video camera, batteries, portable hard drive, adaptor cables, cases, studio lighting equipment, accessories |
Contact: Georgia Rhodes
Location: Waterman Building »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM)
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
---|---|---|
Aquatic System | Marine Ecological Habitats Closed-System Aquarium | 560 L closed-system recirculating marine holding tanks with temperature control (2 units) |
Microscope | Phenom G2Pro Scanning Electron Microscope (SEM) | Desktop Scanning Electron Microscope with ProSuite tiling and remote access software |
Microscope | Olympus SZX16 Stereomicroscope | Light microscope with 12.5 MP Jenoptik progressive scan camera, computer workstation, graphics and imaging software |
Microscope | Olympus IX51 Inverted Fluorescent/Brightfield Microscope | Fluorescence/brightfield microscope with 12.5 MP Jenoptik progressive scan camera, computer workstation, graphics and imaging software |
Microscope | Olympus CH30 Upright Light Microscope | Upright light microscope with 5 MP Jenoptik digital camera, Canon Vixia HFS20 HD video camera, computer workstation, graphics and imaging software (2 units) |
Microscope | Olympus SZ30 Stereomicroscope | Light microscope with LED illuminator (24 units) |
GPS Unit | Trimble Geo 7x Subfoot GPS | Survey-grade high resolution handheld GPS unit with sub-foot accuracy |
GPS Unit | Garmin eTrex 30 (10 units) | Mapping grade gps unit for student use |
Imaging System - Videocamera - High Speed | Edgertronic - monochrome | High speed videocamera for capturing slow motion footage. Capable of Resolutions up to 1280x1024, frame rates at lower resolution up to nearly 18,000 fps |
Imaging System - Videocamera - High Speed | Edgertronic - color | High speed videocamera for capturing slow motion footage. Capable of Resolutions up to 1280x1024, frame rates at lower resolution up to nearly 18,000 fps |
Imaging System - 3D Scanner | Artec Spider | High resolution 3D scanner designed specifically for CAD users |
Imaging System - 3D Scanner | Structure Sensor | Scanners (3) for use with iPad Air, capable of 3D scanning objects and entire rooms |
Workstation, Computer Geographic Information System (GIS) | EPC workstations (3) | Computer workstations outfitted with ArcGIS, auto cad and the full adobe creative suite |
Contact: John W. Morgan, Ph.D.
Location: Roger Williams Medical Center »See on the MapAffiliated Centers: Roger Williams Medical Center Core Research Facilities
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Flow Cytometry Core Contact: Flow Cytometry Core Webpage | Flow Cytometry and Cell Sorting | The Flow Cytometry Core facility is dedicated to advancing research projects requiring cell sorting and/or analysis of cell populations by flow cytometry. The core offers training on and access to shared use instrumentation for analytical flow cytometry, services for fluorescence activated cell sorting (FACS) as well as technical consultation and support on experimental design, execution, and data analysis. |
Imaging Core Imaging Core Webpage | Zeiss LSM 700 confocal, Zeiss Axioplan 2 optical, Zeiss Axiovert 200M optical, IVIS Model 100 | The Imaging Core facility provides technical and logistical access for fixed and live cell fluorescence imaging via visual and digital fluorescent microscopes. This core also offers the ability to visualize fluorescent and bioluminescent markers in live animals via an IVIS imager. |
Molecular Core Molecular Core Webpage | Applied Biosystem PRISM 7000 Sequence Detection System, Bio-Rad ChemiDoc Molecular Imager, Bio-Rad CFX96 Touch Real-Time PCR Detection System | The molecular core offers the capability of performing PCR analysis, quantitative real-time PCR analysis, and digital gel documentation. |
Cell Immunotherapy and Gene Therapy (CITGT) Good Manufacturing Practice (GMP) Facility Contact: Steven C. Katz, MD, FACS (GMP) Facility Webpage | Services and Equipment | The Cell Immunotherapy and Gene Therapy GMP facility supports the manufacture of investigational new drug (IND) products for principal investigators under FDA rules and regulations. |
Tissue Bank Core Tissue Bank Core Webpage | Biospecimen Banking - Tissue Banking and Specimen access | The Tissue Repository Core (TRC) is designed to facilitate translational cancer research by procuring and storing diseased and normal control tissues, with the associated clinical data, for RWMC investigators and outside clients. |
Animal Services Core Animal Services Core Webpage | Husbandry, Surgical Procedures and Injections | The Animal Care Facility is fully maintained, certified, and is supervised by a certified veterinarian, and our Office of Research Administration. |
Contact: Nancy Breen
Location: Marine and Natural Sciences »See on the MapAffiliated Centers: Rhode Island Consortium for Coastal Ecology Assessment Innovation and Modeling (RI C-AIM), IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Fluorescence Activated Cell Sorter (FACS) Contact: Marcie Marston | Millipore Guava easyCyte Flow Cytometer | Cell sorting and analysis |
Gas Chromatograph - Mass Spectrometer (GC - MS) Contact: Nancy Breen | Agilent 5890 Gas Chromatograph - Mass Spectrometer (GC - MS) wth Mass Selective Detetor (MSD) | GC MS for teaching and research |
High Performance Liquid Chromatography (HPLC) Contact: Nancy Breen | Waters 2695 High Performance Liquid Chromatograph-MS / ZQ Micromass Mass Spectrometer | LC - MS for teaching and research |
Gas Chromatograph Contact: Nancy Breen | Agilent 7890 Gas Chromatography with DB-23 column | GC for fatty acid analysis |
Contact: Paul Bertone, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Advise investigators with experimental design for single cell analysis including implementation of technical plans as well as analysis of results. Advise on single cell genomics, RNA-seq, ChIP-seq and bioinformatic analysis. Provide support for grant submissions. |
Contact: Jennifer Friedman, MD, PhD
Location: 55 Claverick Street »See on the MapAffiliated Centers: Center for International Health Research
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Technical Assistance - Statistics and Data Management Contact: Sangshin Park, PhD | Service | This Core provides consultation and assistance in managing and analyzing research data, such as the large datasets, and in linking these data with clinical predictors and outcomes collected in the field. The Core also performs more sophisticated data analytic approaches, including hierarchical, repeated measures modeling and Principal Components Analysis. |
Contact: Mandar T Naik, PhD
Location: Laboratories for Molecular Medicine »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker 500 Avance II | An NMR spectrometer optimized for studying proteins in solution. This spectrometer is most useful for tracking binding interactions, measuring dynamic properties of proteins, and assigning protein resonances. Capable of using 5 mm and 3 mm tubes. Also suitable for proton NMR of small molecules and natural products. |
Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker 850 MHz Avance III HD, with TCI cryoprobe | An NMR spectrometer optimized for studying proteins in solution. This spectrometer is ideal for determining protein structures and measuring protein dynamics. Its extreme sensitivity allows studies on otherwise intractable samples. Capable of using 5 mm, 3 mm, and shaped NMR tubes. Also suitable for proton NMR of small molecules and natural products |
X-Ray Diffraction System | Rigaku ACTOR robot and Saturn 944 HG CCD | An instrument useful for obtaining X-ray diffraction patterns of proteins and other biomacromolecules under cryogenic or room-temperature conditions. Rapid screening and sorting of up to 80 candidate crystals is possible. |
Nuclear Magnetic Resonance (NMR) Spectrometer | Bruker AVANCE III HD 600 MHz High Performance Digital NMR Spectrometer | Bruker NEO 600 MHz NMR spectrometer for high resolution solution-state protein NMR experiments 4 RF channels, triple-resonance (1H-13C-15N) inverse cryoprobe with cooled 1H, 13C and 15N preamplifiers. |
Contact: Bongsup Cho, Ph.D.
Location: Pharmacy Building »See on the MapAffiliated Centers: IDeA Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) at University of Rhode Island
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Imaging System - 3D Printer | Z650 3D System/Z-corp | Produces realistic color (Powder Based) models without paint to better evaluate the look, feel, and style of product designs. Five print heads provide the widest range of accurate colors, including true black and improved color consistency from part to part. 3D print text labels or images can be directly applied onto printed models. |
Contact: Attila Seyhan, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Training and Technical Assistance | Service | Advise investigators on study design leading to translational clinical protocols. For prioritized studies, provide assistance with clinical protocol preparation as well as NIH, Industry or Foundation grant submissions to secure funding for the research. |
Contact: Murray Resnick, MD, PhD
Affiliated Centers: Legorreta Cancer Center
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Contact: Simon Vojta
Location: Narragansett Bay Campus - Graduate School of OceanographyAffiliated Centers: Sources, Transport, Exposure and Effects of PFAS (STEEP) Superfund Research Program
Instrument/Service/Resource Unit | Manufacturer/Description | Function |
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Mass Spectrometer Contact: Simon Vojta Mass Spectrometer Available to Researchers | SCIEX X500R QTOF PLATFORM coupled to an EXION LC AC SYSTEM | A tandem (MS/MS) mass spectrometer combining a quadrupole and a time-of-flight analyzer allows collecting high-resolution MS and MRM spectra. The instrument is suitable for both quantitative analysis as well as non-targeted approach and operates under SCIEX OS software. The collected data are evaluated using an independent processing station (SCIEX Alpha Workstation 2020) equipped with SCIEX OS-Q software |
Contact: Beth Brainerd, PhD
Location: BioMedical Center Building »See on the MapInstrument/Service/Resource Unit | Manufacturer/Description | Function |
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Imaging System - Computed Tomography (CT) Scanner | Animage Fidex Veterinary | Veterinary CT Scanner designed for animals in the size range from rats to dogs. In vivo scanning of anesthetized animals is possible. |
Imaging System - GE Lightspeed 16 Slice CT Scanner | GE | |
Imaging System - Videoradiography Room XROMM | Imaging Systems and Services | Custom designed biplanar videoradiography system for XROMM studies of human and animal subjects. |
Imaging system X-Ray Fluoroscopes Contact: XROMM | Radiological Imaging Mobile C-Arm X-ray | Two retrofitted with 30-cm image intensifiers and high-speed video cameras for measuring accurate 3D skeletal kinematics of small to medium-sized animals. |
Imaging System - MicroComputed Tomography (uCT) Scanner | Bruker BioSpin | SkyScan 1276 in vivo microCT scanner for small animals (mouse to rat size) |