1999 Syllabus
Pfizer Bio154A (Molecular Genetics)
Instructor: Dr. E. A. Hendrickson JWW Rm 162 863-3667 e-mail: Eric_Hendrickson@brown.edu
TA: Robyn Tanny JWW Rm 110 863-1726 e-mail: Robyn_Tanny@brown.edu
TA: Stephen Dunaway JWW Rm 110 863-1726 e-mail: Stephen_Dunaway@brown.edu
TA: Xiaolan Hu JWW Rm 110 863-1726 e-mail: Xiaolan_Hu@brown.edu
REQUIRED TEXTS: Recombinant DNA (2nd Edition), Watson et al., (1992), W. H. Freeman & Co.
Hannah's Heirs, Daniel Pollen, (1993), Oxford Press
Racing to the Beginning of the Road, Robert Weinberg, (1996), W. H. Freeman & Co.
I
1. Sep. 8
Introduction
Chapter 1 Establishing the role of genes within cells
Chapter 2 DNA is the primary genetic material
Chapter 3 Elucidation of the genetic code
Chapter 4 The genetic elements that control gene expression
2. Sep. 15
Chapter 8 The complexity of the genome
Chapter 9 Controlling eukaryotic gene expression
Chapter 5 Methods of creating recombinant DNA molecules-"cloning"
Chapter 7 The isolation of cloned genes-1: Immunoscreening (Hodgkin's Disease)
------------- PROBLEM SET I HANDED OUT
3. Sep. 22
Chapter 7 The isolation of cloned genes-2: Panning (Hodgkin's Disease/CD75)
Chapter 7 The isolation of cloned genes-3: Functional complementation I (Fanconi's Anaemia)
------------- PROBLEM SET I DUE BACK
------------- PROBLEM SET II HANDED OUT
4. Sep. 29
Chapter 6 The polymerase chain reaction (PCR): cCcancer
Chapter 11 In vitro mutagenesis-1: HIV tat
------------- PROBLEM SET II DUE BACK
------------- TAKE HOME EXAM I HANDED OUT
II
5. Oct. 6
Chapter 11 In vitro mutagenesis-2: HIV tat
Chapter 12 Transferring genes into mammalian cells-1: Oct-3
Chapter 13 Using yeast to study eukaryotic gene function-1: Complementation (cyclin)
------------- TAKE HOME EXAM I DUE BACK
------------- PROBLEM SET III HANDED OUT
6. Oct. 13
Chapter 13 Using yeast to study eukaryotic gene function-2: Complementation/two hybrid system
Chapter 16 Molecules of immune recognition-1: V(D)J recombination
Chapter 16 Molecules of immune recognition-2: RAG-1
------------- PROBLEM SET III DUE BACK
7. Oct. 20
Chapter 16 Molecules of immune recognition-3: RAG-1/RAG-2
------------- Sex determination-1: ZFY
------------- Sex determination-2: SXR
------------- PROBLEM SET IV HANDED OUT
Oct. 27 NO CLASS
8. Nov. 3
------------- Sex determination-3: SXR
Chapter 14 The introduction of foreign genes into mice-1: Transgenics (SXR, Inv)
------------- PROBLEM SET IV DUE BACK
------------- TAKE HOME EXAM II HANDED OUT
III
9. Nov. 10
Chapter 14 The introduction of foreign genes into mice-2: Knockouts (RAG-2, CaMK)
Chapter 14 The introduction of foreign genes into mice-3: Conditional Knockouts (
b-pol)------------- TAKE HOME EXAM II DUE BACK
10. Nov. 17
Chapter 29 Studying whole genomes: YACS
Chapter 26 Mapping and cloning human disease genes-1: Fragile X
------------- PROBLEM SET V HANDED OUT
11. Nov. 24
Chapter 26 Mapping and cloning human disease genes-2: HD, BRCA-1/2
------------- PROBLEM SET V DUE BACK
12. Dec. 1
Chapter 26 Mapping and cloning human disease genes-3: Ob, AD
Chapter 28 Working toward human gene therapy: CF
------------- PROBLEM SET VI HANDED OUT
13. Dec. 8
Chapter 30 The human genome initiative-Finding all the human genes
------------- PROBLEM SET VI DUE BACK
14. Dec. 15
FINAL EXAM