Investigation is aimed at elucidating the mechanisms that cells employ in regulating their volume under normal conditions and during osmotic stress. We are examining this question in fish, since many representatives of this group normally encounter wide variations in osmolarity of the external environment and can tolerate marked perturbations in the osmotic pressure of their extracellular fluid.

Biography

Leon Goldstein, Ph.D. was born in Malden, Massachusetts. He received his B.S. degree from Northeastern University in 1954, his Ph.D. from Boston University in 1958, and his M.A. (Hon.) from Oxford University in 1983. He has held academic posts at Dartmouth College, Harvard Medical School, Brown University, and a visiting professorship at Oxford University in England.

Dr. Goldstein is currently a Professor of Medical Science and the Vice-Chairman of the Department of Molecular Pharmacology, Physiology and Biotechnology at Brown University. He has received continuous funding for his research since 1962. He lives in Providence, Rhode Island with his wife Barbara and has three children.

Institutions

Bu

Research Description

Investigation is aimed at elucidating the mechanisms that cells employ in regulating their volume under normal conditions and during osmotic stress. We are examining this question in fish, since many representatives of this group normally encounter wide variations in osmolarity of the external environment and can tolerate marked perturbations in the osmotic pressure of their extracellular fluid. Current studies focus on the role of organic solutes in maintaining cell volume during disturbances in extracellular fluid osmolarity. Membrane transport mechanisms involved in modulating the cellular concentrations of these organic solutes during osmotic stress are under investigation. The role of cell signaling and membrane trafficking in regulating organic solute transport systems during osmotic stress is being elucidated.

Awards

• Career Development Awardee, National Institute of Health, 1963-1971
• Elected Fellow of the American Association for the Advancement of Science, 1965
• Fogarty Senior International Fellowship, U.S. Public Health Service, 1983
• Visiting Research Fellow, Merton College, Oxford University, 1983
• IBM Lectureship, Williams College, 1985
• Alvin F. Rieck Lectureship, Medical College of Wisconsin, 1986
• Burroughs Wellcome Visiting Professor, Department of Biochemistry, Oxford University, 1989
• Member, Senior Common Room, Merton College, Oxford, 1989-1994.
• Elizabeth Dunham Distinguished Lectureship, Dartmouth Medical School, 2004.

Affiliations

American Physiological Society
Society of General Physiologists
American Chemical Society
American Society of Zoologists
American Association for the Advancement of Science

Funded Research

NIH R01: continuous support 1962-1987
NSF research grant: continuous support 1962-present

Teaching Experience

Dr. Goldstein has been teaching at the university level since 1959.

Courses Taught

• Introduction to Human Physiology (BI0006)
• Principles of Exercise Physiology (BI0116)

View My Full Publication List in pdf format

Selected Publications

• Musch, Mark W., Goldstein, Leon. Tyrosine Kinase Inhibition Affects Skate Anion Exchanger Isoform I Alterations After Volume Expansion. Am. J. Physiol. Regul. Integr. Comp. Physiol. 288: R885-R890, 2005.(2005)
• Koomoa, Dana-Lynn T., Musch, Mark W., Goldstein, Leon. Osmotic Stress Stimulates the Organic Osmolyte Channel in Xenopus Laevis Oocytes Express Skate (Raja Erinacea) AE1. J. Exp. Zoology. 303A: 319-322, 2005.(2005)
• Perlman, D. F., Goldstein, L. The Anion Exchanger as an Osmolyte Channel in the Skate Erythrocyte. Neurochem Res., 29 (1): 9-15, 2004.(2004)
• Koomoa, D-L.T., Musch, M.W., Myers, D.E., Goldstein, L. Expression of the Skate (Raja erinacea) AE1 Osmolyte Channel in Xenopus laevis Oocytes: Monovalent Cation Permeability. J. Membrane Biol. 198: 23-29, 2004.(2004)
• Musch, Mark W., Koomoa, Dana-Lynn T., Goldstein, Leon. Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1. Role of Lipid Raft Regions. J. Biol. Chem. 279: 39447-39453, 2004.(2004)
• Koomoa, D. –L.T., Musch, M.W., Goldstein, L. Comparison of the Osmolyte Transport Properties Induced by trAE1 versus I Clswel in Xenopus Oocytes. J. Membrane Biol. 185:57-63, 2003.(2003)
• Goldstein, L., Koomoa, D-L., Musch, M.W. ATP Release From Hypotonically Stressed Skate RBC: Potential Role in Osmolyte Channel Regulation. J. Exp. Zoology. 296A: 160-163, 2003(2003)
• Guizouarn, H., Musch, M.W., Goldstein, L. Evidence for the Presence of Three Different Anion Exchangers in a Red Cell. Functional Expression Studies in Xenopus Oocytes. J. Membrane Biol. 193: 109-120, 2003.(2003)
• Davis, E.M., Musch, M.W., Goldstein, L. Transfection of an Inducible Trout Anion Exchanger (AE1) Into HEK-EcR Cells. J. Exp. Zoology. 293:46-57, 2002.(2002)
• Musch, M.W., and Goldstein, L. Modulation of Erythrocyte Band 4.1 Binding by Volume Expansion. J. Exp. Zoology. 289:177-183, 2001.(2001)
• Koomoa, D-L., Musch, M.W., Maclean, A., Goldstein, L. Volume-activated trimethylamine oxide efflux in red blood cells of spiny dogfish (Squalus acanthias). Am J Physiol 281: R803-R810, 2001.(2001)
• Hubert, E.M., Musch, M.W. and Goldstein, L. Inhibition of volume-stimulated taurine efflux and tyrosine kinase activity in the skate red blood cell. Eur. J. Physiol. 440: 132 – 139, 2000.(2000)
• Wittles, K.A., Hubert, E.M., Musch, M.W., and Goldstein, L. Osmolyte channel regulation by ionic strength in skate RBC. Am. J. Physiol. 279: R69 – R76, 2000.(2000)
• Musch, M.W., Hubert, E.M. and Goldstein L. Volume expansion stimulates p72syk and p56lyn in skate erythrocytes. J. Biol. Chem. 274 (12): 7923 – 7928, 1999.(1999)
• Musch, M.W., Davis-Amaral, E.M., Vandenburgh, H.H. and Goldstein, L. Hypotonicity stimulates translocation of Icln in neonatal rat cardiac myocytes. Pflugers Arch – Eur. J. Physiol. 436: 415 – 422, 1998.(1998)
• Musch, M.W., Davis-Amaral, E.M., Leibowitz, K.L. and Goldstein, L. Hypotonic-stimulated taurine efflux in skate erythrocytes: regulation by tyrosine phosphatase activity. Am. J. Physiol. 274: R1677-R1686, 1998.(1998)
• Musch, M.W. and Goldstein, L. High affinity binding of ankyrin induced by volume expansion in skate erythrocytes. J. Biol. Chem. 271(35): 21221-21225, 1996.(1996)