The work in the Boekelheide laboratory concerns the mechanisms of testicular injury induced by toxic industrial and environmental compounds, mixtures of contaminants, and the reproductive/developmental consequences of exposure to endocrine disruptors.

Biography

Kim Boekelheide is Professor of Pathology and Laboratory Medicine at the Brown University School of Medicine. He received his B.A. from Harvard University, and M.D. and Ph.D. from Duke University. His research examines fundamental molecular mechanisms by which environmental and occupational toxicants induce testicular injury. Current projects include the study of co-exposure synergy using model testicular toxicants and the effects of in utero endocrine disruptor exposure on steroidogenesis and a predisposition to cancer. He is Director of the Brown University Superfund Research Program and Children's Environmental Health Center. His research has been continuously funded by the National Institute of Environmental Health Sciences since 1985 and he has received several awards including a Burroughs Wellcome Toxicology Scholar Award (1994-1999).

Institutions

Duke University

Research Description

The research in the Boekelheide laboratory examines fundamental molecular mechanisms by which environmental and occupational toxicants induce testicular injury. Current projects include the study of co-exposure synergy using model testicular toxicants, the biological basis of irreversible testicular atrophy focusing on pathways of germ cell apoptosis and proliferation, the role of in utero exposure to endocrine disruptors in the induction of testicular germ cell cancer, and the testing of human-relevant model systems for the response to anti-androgenic endocrine disruptors including phthalates.

Awards

Doerenkamp-Zbinden Award, 2009 (given to authors of the National Academy of Sciences report entitled Toxicity Testing in the 21st Century: A Vision and a Strategy)
Center for Alternatives to Animal Testing (CAAT) Recognition Award, 2009 (given to authors of the National Academy of Sciences report entitled Toxicity Testing in the 21st Century: A Vision and a Strategy)
AstraZeneca Traveling Lectureship Award, Society of Toxicology, 2009
Annual Best Paper Award in Experimental Biology, 2002, Experimental Biology & Medicine
Burroughs Wellcome Toxicology Scholar Award, 1994-1999
Research Career Development Award, 1989 - 1994
International Life Sciences Institute Research Foundation Award in Comparative Toxicology, 1987- 1990
Pharmaceutical Manufacturers Association Foundation Faculty Development Award in Toxicologic Pathology, 1985-1987
Medical School Senior Citation, 1989
Sigma Xi, Duke University, 1981
Phi Beta Kappa, Duke University, 1980
Alpha Omega Alpha, Duke University, 1977

Affiliations

Society of Toxicology, 1985
American Society of Andrology, 1986
American Society for Cell Biology, 1986
American Society for Investigative Pathology, 1986
American Association for the Advancement of Science, 1987
American Chemical Society, 1988
Society for the Study of Reproduction, 1990

Funded Research

R01 ES017272 "Molecular Mechanism of Human Fetal Testis Susceptibility to Endocrine Disruption," P.I. Kim Boekelheide, 25% effort (first year direct costs, $341,293), 01/14/10-11/30/14

P20 ES018169 "Formative Center for the Evaluation of Environmental Impacts on Fetal Development," P.I. Kim Boekelheide, 20% effort (first year direct costs, $499,028), 01/01/10-12/31/12

P42ES013660 Superfund Research Program (NIEHS) "Reuse in RI: A State-based Approach to Complex Exposures," P.I. Kim Boekelheide, 25% effort (direct costs, ~$2.0M per year), 04/01/05-03/31/14

P42ES013660 Superfund Research Program (NIEHS), Project 1: Testicular Sensitization and Co-Exposure Synergy, P.I. Kim Boekelheide, 10% effort (direct costs, ~$165,000 per year), 04/01/05-03/31/14

P42ES013660-05S1 Superfund Research Program (NIEHS) supplement. This is a supplement under ARRA for the collaboration between Brown University (Project 8 Carmen Marsit) and the Dartmouth College (Project 4-PI: Marget Karagas) that will utilize population-based birth cohort studies to examine the impact of environmental exposures at levels commonly observed in the US population and adverse pregnancy outcomes, specifically growth restriction and preterm birth. P.I. Kim Boekelheide (direct costs, ~149,328 per year), 09/06/2009 – 8/31/2011

P42ES013660-05S2 Superfund Research Program (NIEHS) supplement. This is a supplement under ARRA for the collaboration between Brown University (Project 7 –Eric Suuberg) and the Boston University (Translational Core –PI: Madeline Scammell) to assess vapor intrusion exposure and risk. P.I. Kim Boekelheide (direct costs, ~$138,501 per year), 09/17/09 – 8/31/11

P42ES013660-05S3 Superfund Research Program (NIEHS) supplement. This is an administrative supplement for the collaboration between Brown University Research Translation Core & Project 7 and the US EPA's National Health and Environmental Effects Research Laboratory . P.I. Kim Boekelheide (direct costs, ~83,047 per year), 04/01/09 – 03/31/11

American Chemical Council, "Development of an inter-species bioassay to test phthalate susceptibility," P.I. Kim Boekelheide, 7% effort (direct costs, $170,233), 02/18/09-02/17/10, to be re-funded for another year

Selected Publications

• Boekelheide, K., Campion, S.N. (2010). Using the New Toxicity Testing Paradigm to Create a Taxonomy of Adverse Effects. Tox. Sci. 114, 20-24 .(2010)
• 98) Boekelheide, K., Kleymenova, E., Liu, K., Swanson, C., Gaido, K.W. (2009). Dose-dependent effects on cell proliferation, seminiferous tubules, and male germ cells in the fetal rat testis following exposure to di (n-butyl) phthalate. Micro. Res. Tech., in press.(2009)
• Paul, E., Cronan, R., Weston, P. J., Boekelheide, K., Sedivy, J.M., Lee, S.Y., Wiest, D.L., Resnick, M.B., Klysik, J.E. (2009). Disruption of Supv3L1 damages the skin and causes sarcopenia, loss of fat, and death. Mamm. Genome 20, 92-108.(2009)
• Thanos C, Emerich D, Bintz B, Goddard M, Mills J, Jensen R, Lombardi M, Hall S, and Boekelheide K (2009). Secreted products from the porcine choroid plexus accelerate the healing of cutaneous wounds. Cell Transplant. 18, 1395-1409.(2009)
• 13. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Bisphenol A. 2008, NIH Publication No. 08-5994.(2008)
• 22. Chapin, R.E., Adams, J., Boekelheide, K., et al. (2008). NTP-CERHR expert panel report on the reproductive and developmental toxicity of bisphenol A. Birth Defects Res B Dev Reprod Toxicol. 83, 157-395.(2008)
• 96) Ryu, K.-Y. Sinnar, S.A. Reinholdt, L.G., Vaccari, S., Hall, S., Garcia, M.A., Zaitseva, T.S., Bouley, D.M., Boekelheide, K., Handel, M.A., Conti, M., Kopito, R.R. (2008). The mouse polyubiquitin gene Ubb is essential for meiotic progression. Mol. Cell, Biol. 28, 1136-46.(2008)
• 95) Bryant, B.H., Yamasaki, H., Sandrof, M., Boekelheide, K. (2008). Spermatid head retention as a marker of 2,5-hexanedione-induced testicular toxicity in the rat. Toxicologic Pathol. 36, 552-559.(2008)
• 94) Klysik, J., Theroux, S., Sedivy, J.M., Moffit, J.S., Boekelheide, K. (2008). Signaling crossroads: The function of Raf Kinase Inhibitory Protein in cancer, the central nervous system and reproduction. Cell. Signal. 20, 1-9.(2008)
• 97) Boekelheide, K., Sigman, M. (2008). Is gene therapy for the treatment of male infertility feasible? Nat. Clin. Pract. Urol., in press.(2008)
• 93) Moffit, J.S., Boekelheide, K., Sedivy, J.M., Klysik, J. (2007). Mice lacking Raf Kinase Inhibitor Protein-1 (RKIP-1) have altered sperm capacitation and reduced reproduction rates with a normal response to testicular injury J. Androl. 28, 883-90.(2007)
• 91) Gaido, K.W., Hensley, J.B., Liu, D. Wallace, D.G., Borghoff, S., Johnson, K.J., Hall, S.J., Boekelheide, K. (2007). Fetal mouse phthalate exposure shows that gonocyte multinucleation is not associated with decreased testicular testosterone. Tox. Sci. 97, 491-503.(2007)
• 88) Rasoulpour, R.J., Boekelheide, K. (2007). NF-κB activation elicited by ionizing radiation is pro-apoptotic in testis. Biol. Reprod. 76, 279-85.(2007)
• 90) Moffit, J.S., Bryant, B.H., Hall, S.J., Boekelheide K. (2007). Dose-dependent effects of Sertoli cell toxicants 2,5-hexanedione, carbendazim, and mono-(2-ethylhexyl) phthalate in adult rat testis. Toxicologic Pathol. 35, 719-27.(2007)
• 89) Bryant, B.H., Boekelheide, K. (2007). Time-dependent changes in post-mortem testis histopathology in the rat. Toxicologic Pathol. 35, 665-71.(2007)
• 92) Boekelheide, K. (2007). Highlight—Mixed Messages. Tox. Sci. 99, 1-2.(2007)
• 12. Toxicity Testing in the Twenty-first Century: A Vision and a Strategy. National Research Council. 2007, National Academy Press, Washington, D.C.(2007)
• 87) Emerich, D.F., Thanos, C.G., Goddard, M., Skinner, S.J., Geany, M.S., Bell, W.J., Bintz, B., Schneider, P., Chu, Y., Babu, R.S., Borlongan, C.V., Boekelheide, K., Hall, S., Bryant, B., Kordower, J.H. (2006). Extensive neuroprotection by choroid plexus transplants in excitotoxin lesioned monkeys. Neurobiol. Dis. 23, 471-80.(2006)
• 86) Rasoulpour, R.J., Boekelheide, K. (2006). The Sycp-1Cre transgenic mouse and male germ cell inhibition of NF -κB. J Androl. 27, 729-733.(2006)
• 21. Bailey, J., et al (Boekelheide, K. listed as 13th author). (2006). Synopsis: 2006 Annual Meeting of the American Society of Andrology. J. Androl. 27, 712-719.(2006)
• 20. Kavlock, R., Barr, D., Boekelheide, K., Breslin, W., Breysse, P., Chapin, R., Gaido, K., Hodgson, E., Marcus, M., Shea, K., Williams, P. (2006). NTP-CERHR Expert Panel Update on the Reproductive and Developmental Toxicity of di(2-ethylhexyl) phthalate. Reprod. Toxicol. 22, 291-399.(2006)
• 11. Flouride in Drinking Water. A Scientific Review of EPA's Standards. National Research Council. 2006, National Academy Press, Washington, D.C.(2006)
• 10. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di(2-Ethylhexyl) Phthalate (DEHP). 2006, NIH Publication No. 06-4476.(2006)
• 19. Boekelheide, K., Johnson, K.J., Richburg, J.H. (2005). Sertoli Cell Toxicants. In Sertoli Cell Biology (eds. Griswold, M.D., Skinner, M.K.). Elsevier Inc, San Diego, pp. 345-382.(2005)
• 85) Bercovitch, R.S., Januario, J.A., Terry, S.F., Boekelheide, K., Podis, A.D., Dupuy, D.E., Bercovitch, L.G. (2005). Testicular microlithiasis in association with pseudoxanthoma elasticum. Radiology 237, 550-554.(2005)
• 83) Beall, S.A., Boekelheide, K., Johnson, K.J. (2005). Hybrid GPCR/cadherin (Celsr) proteins in rat testis are expressed with cell type-specificity and exhibit differential Sertoli-germ cell adhesion activity. J. Androl. 26, 529-538.(2005)
• 79) Boekelheide, K., Schoenfeld, H.A., Hall, S.J., Weng, C.C., Shetty, G., Leith, J., Harper, J.S., Sigman, M., Hess, D.L., Meistrich, M.L. (2005). Gonadotropin-releasing hormone antagonist (Cetrorelix) therapy fails to protect non-human primates (Macaca arctoides) from radiation-induced spermatogenic failure. J. Androl. 26, 222-34.(2005)
• 82) Boekelheide, K. (2005). Mechanisms of toxic damage to spermatogenesis. J. Natl. Cancer Inst. Monogr. 34, 1-3.(2005)
• 81) Augustine, L.M., Markelewicz, R.J., Boekelheide, K., Cherrington, N.J. (2005). Xenobiotic and endobiotic transporter mRNA expression in the blood-testis barrier. Drug Metab. Dispos. 33, 182-189.(2005)
• 84) Kleymenova, E., Liu, K., Swanson, C., Boekelheide, K., Gaido, K.W. (2005). Exposure in utero to di(n-butyl)phthalate alters the vimentin cytoskeleton of fetal rat Sertoli cells and disrupts Sertoli cell-gonocyte contact. Biol. Reprod. 73, 482-490.(2005)
• 80) Rasoulpour, R.J., Boekelheide, K. (2004). NF-B is activated in the rat testis following exposure to mono-(2-ethylhexyl) phthalate. Biol. Reprod. 72, 479-486.(2004)
• 78) Boekelheide, K. (2004). Highlight—Cracking the nut. Tox. Sci. 81, 1-2.(2004)
• 77) Markelewicz, R.J., Hall, S.J., Boekelheide, K. (2004). 2,5-Hexanedione and carbendazim co-exposure synergistically disrupts rat spermatogenesis despite opposing molecular effects on microtubules. Tox. Sci. 80, 92-100.(2004)
• 18. Boekelheide, K., Darney, S.P., Daston, G.P., David, R.M., Luderer, U., Olshan, A.F., Sanderson, W.T., Willhite, C.C., Woskie, S. (2004). NTP-CERHR Expert Panel Report on the reproductive and developmental toxicity of 2-bromopropane. Reprod. Toxicol. 18, 189-217.(2004)
• 9. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-n-Hexyl Phthalate (DnHP). 2003, NIH Publication No. 03-4489.(2003)
• 6. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-Isodecyl Phthalate (DIDP). 2003, NIH Publication No. 03-4485.(2003)
• 75) Hixon, M.L., Boekelheide, K. (2003). Expression and localization of total Akt1 and phosphorylated Akt1 in the rat seminiferous epithelium. J. Androl. 24, 891-898.(2003)
• 73) Fleming, S.L., Shank, P.R., Boekelheide, K. (2003). -Tubulin overexpression in Sertoli cells in vivo. I. Localization to sites of spermatid head attachment and alterations in Sertoli cell microtubule distribution. Biol. Reprod. 69, 310-321.(2003)
• 72) McBurney M.W., Yang, X., Jardine, K., Hixon, M.L., Boekelheide, K., Webb, J., Lansdorp, P.M., Lemeiux,M. (2003). The mammalian Sir2 alpha protein has a role in embryogenesis and gametogenesis. Mol. Cell Biol. 23, 38-54.(2003)
• 74) Fleming, S.L., Shank, P.R., Boekelheide, K. (2003). -Tubulin overexpression in Sertoli cells in vivo. II. Retention of spermatids, residual bodies, and germ cell apoptosis. Biol. Reprod. 69, 322-330.(2003)
• 76) Rasoulpour, R.J., Schoenfeld, H.A., Gray, D.A., Boekelheide, K. (2003). Animal Model – Expression of a K48R mutant ubiquitin protects mouse testis from cryptorchid injury and aging. Am. J. Pathol. 163, 2595-2603.(2003)
• 5. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-Isononyl Phthalate (DnIP). 2003, NIH Publication No. 03-4484.(2003)
• 4. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of 2-Bromopropane. 2003, NIH Publication No. 03-4480.(2003)
• 3. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of 1-Bromopropane. 2003, NIH Publication No. 03-4479.(2003)
• 8. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-n-Octyl Phthalate (DnOP). 2003, NIH Publication No. 03-4488.(2003)
• 7. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Butyl Benzyl Phthalate (BBP). 2003, NIH Publication No. 03-4487.(2003)
• 2. Boekelheide, K., and Chapin, R.E. (2002). Guest Editors of Chapter 16: Male Reproductive Toxicology. In Current Protocols in Toxicology (ed., Maines, M.D.). Wiley, New York.(2002)
• 15. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-n-hexyl phthalate. Reprod. Toxicol. 16, 709-719.(2002)
• 12. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di(2-ethylhexyl) phthalate. Reprod. Toxicol. 16, 529-653.(2002)
• 71) Embree-Ku, M.E., Boekelheide, K. (2002). FasL deficiency enhances the development of tumors in p53+/- mice. Toxicol. Pathol. 30, 705-713.(2002)
• 68) Johnson, K.J., Boekelheide, K. (2002). Dynamic testicular adhesion junctions are immunologically unique. II. Localization of classic cadherins in rat testis. Biol. Reprod. 66, 992-1000.(2002)
• 67) Johnson, K.J., Boekelheide, K. (2002). Dynamic testicular adhesion junctions are immunologically unique. I. Localization of p120 catenin in rat testis. Biol. Reprod. 66, 983-991.(2002)
• 70) Embree-Ku, M.E., Boekelheide, K. (2002). Absence of p53 and FasL has sexually dimorphic effects on both development and reproduction. Exp. Biol. Med. 227, 545-553.(2002)
• 69) Embree-Ku, M.E., Venturini, D., Boekelheide, K. (2002). Fas is involved in the p53-dependent apoptotic response to ionizing radiation in mouse testis. Biol. Reprod. 66, 1456-1461.(2002)
• 11. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-n-butyl phthalate. Reprod. Toxicol. 16, 489-527.(2002)
• 10. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of butyl benzyl phthalate. Reprod. Toxicol. 16, 453-487.(2002)
• 14. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-isononyl phthalate. Reprod. Toxicol. 16, 679-708.(2002)
• 13. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-isodecyl phthalate. Reprod. Toxicol. 16, 655-678.(2002)
• 17. Boekelheide, K., Fleming, S.L., Allio, T., Embree-Ku, M.E., Hall, S.J., Johnson, K.J., Kwon, E.J., Patel, S.R., Rasoulpour, R.J., Schoenfeld, H.A., Thompson, S. (2002). 2,5-Hexanedione-induced testicular injury. Ann. Rev. Pharmacol. Toxicol. 43, 125-147.(2002)
• 16. Kavlock, R., Boekelheide, K., Chapin, R.E., Cunningham, M. Faustman, E. et al. (2002). NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-n-octyl phthalate. Reprod. Toxicol. 16, 721-734.(2002)
• 9. Boekelheide, K., Schoenfeld, H.A. (2001). Spermatogenesis by Sisyphus: Proliferating stem germ cells fail to repopulate the testis after 'irreversible' injury. In Biological Reactive Intermediates VI: Chemical and Biological Mechanisms in Susceptibility to and Prevention of Environmental Disease (eds., Dansette, P.M., et al.). Plenum Publishing, New York, pp. 421-428.(2001)
• 66) Schoenfeld, H.A., Hall, S.J., Boekelheide, K. (2001). Continuously proliferative stem germ cells partially re-populate the aged, atrophic rat testis after GnRH agonist therapy. Biol. Reprod. 64, 1273-1282.(2001)
• 2. NTP-CERHR Monograph on the Potential Human Reproductive and Developmental Effects of Di-n-Butyl Phthalate (DBP). 2000, NTP-CERHR-DBP-00.(2000)
• 64) Fox, R.A, Sigman, M., Boekelheide, K (2000). Transmembrane versus soluble stem cell factor expression in human testis. J. Androl. 21, 579-585.(2000)
• 62) Richburg, J.H., Nañez, A., Williams, L.R., Embree, M.E., Boekelheide, K. (2000). Sensitivity of testicular germ cells to toxicant-induced apoptosis in gld mice that express a non-functional form of FasL. Endocrinology 141, 787-793.(2000)
• 63) Johnson, K.J., Patel, S.R., Boekelheide, K. (2000). Multiple cadherin superfamily members with unique expression profiles are produced in rat testis. Endocrinology 141, 675-683.(2000)
• 65) Roehl, H.H., Leibbrandt, M.E.I., Greengard, J.S., Kamantigue, E., Glass, W.G., Giedlin, M., Boekelheide, K., Johnson, D.E., Jolly, D.J., Sajjadi, N.C. (2000). Analysis of testes and semen from rabbits treated by intravenous injection with a retroviral vector encoding the human factor VIII gene—no evidence for germline transduction. Hum. Gene Ther. 11, 2529-2540.(2000)
• 8. Embree, M.E., Boekelheide, K. (2000). Germ cell apoptosis. In Testis, Epididymis and Technologies in the Year 2000; 11th European Workshop on Molecular and Cellular Endocrinology of the Testis (eds., Jegou, B. et al.). Springer, Berlin, pp. 141-163.(2000)
• 7. Boekelheide, K., Fleming, S.L., Johnson, K.J., Patel, S.R., Schoenfeld, H.A. (2000). Role of Sertoli cells in injury-associated testicular germ cell apoptosis. Proc. Soc. Exptl. Biol. Med. 225, 105-115.(2000)
• 61) Lee, J, Richburg, J.H., Shipp, E.B., Meistrich, M.L., Boekelheide, K. (1999). The Fas system, a regulator of testicular germ cell apoptosis, is differentially upregulated in Sertoli cell versus germ cell injury of the testis. Endocrinology 140, 852-858.(1999)
• 60) Varmuza, S., Jurisicova, A., Okano, K., Hudson, J., Boekelheide, K., Shipp, E.B. (1999). Spermiogenesis is impaired in mice bearing a targeted mutation in the protein phosphatase 1c gene. Dev. Biol. 205, 98-110.(1999)
• 6. Boekelheide, K. (1998). Letter to the Editor: Response to Guest Editorial by Philip G. Watanabe. Toxicol. Sci. 46, 418-419.(1998)
• 59) Boekelheide, K., Lee, J., Shipp, E.B., Richburg, J.H., Li, G. (1998). Expression of Fas system-related genes in the testis during development and after toxicant exposure. Toxicol. Lett. 102-103, 499-504.(1998)
• 57) Blanchard, K.T., Lee, J., Boekelheide, K. (1998). Leuprolide, a gonadotropin-releasing hormone agonist, reestablishes spermatogenesis after 2,5-hexanedione-induced irreversible testicular injury in the rat resulting in normalized stem cell factor expression. Endocrinology 139, 236-244.(1998)
• 58) Boekelheide, K., Hall, S.J., Richburg, J.H. (1998). In vitro evaluation of Sertoli cell toxicants which target microtubules and decrease seminiferous tubule fluid formation. In Vitro Toxicol. 11, 309-314.(1998)
• 1. Gender Differences in Susceptibility to Environmental Factors: A Priority Assessment (eds., Setlow, V.P., Lawson, C.E., and Woods, N.F.). 1998, National Academy Press, Washington, D.C.(1998)
• 55) Lee, J., Richburg, J.H., Younkin, S.C., Boekelheide, K. (1997). The Fas system is a key regulator of germ cell apoptosis in the testis. Endocrinology 138, 2081-2088.(1997)
• 53) Jiang, C., Hall, S.J., Boekelheide, K. (1997). Cloning and characterization of the 5´ flanking region of the stem cell factor gene in rat Sertoli cells.Gene 185, 285-290.(1997)
• 52) Blanchard, K.T., Boekelheide, K. (1997). Adenovirus-mediated gene transfer to rat testis in vivo. Biol. Reprod. 56, 495-500.(1997)
• 54) Costa, S.L., Boekelheide, K., Vanderhyden, B.C., Seth, R., McBurney, M.W. (1997). Male infertility caused by epididymal dysfunction in transgenic mice expressing a dominant negative mutation of retinoic acid receptor . Biol. Reprod. 56, 985-990.(1997)
• 56) Jiang, C., Hall, S.J., Boekelheide, K. (1997). Development and characterization of a tumorigenic rat Sertoli cell line, 93RS2. J. Androl. 18, 393-399.(1997)
• 5. Richburg, J.H., Blanchard, K.T., and Boekelheide, K. (1997). Sertoli Cell. In Comprehensive Toxicology, Volume 10: Reproductive and Endocrine Toxicology, Section i: Male Reproductive Toxicology (eds., Sipes, I.G., McQueen, C.A., and Gandolfi, A.J.). Elsevier Science, Oxford, pp. 127-138.(1997)
• 4. Cohen, S.D., Pumford, N.R., Khairalah, E.A., Boekelheide, K., Pohl, L.R., Amouzadeh, H.R., Hinson, J.A. (1997). Selective protein covalent binding and target organ toxicity. Toxicol. Appl. Pharmacol. 143, 1-12.(1997)
• 1. Boekelheide, K., and Chapin, R.E. (1997). Editors of Section 1: Male Reproductive Toxicology. In Comprehensive Toxicology, Volume 10: Reproductive and Endocrine Toxicology (eds., Sipes, I.G., McQueen, C.A., and Gandolfi, A.J.). Elsevier Science, Oxford, pp. 1-247.(1997)
• 51) Allard, E.K., Blanchard, K.T., Boekelheide, K. (1996). Exogenous stem cell factor (SCF) compensates for altered endogenous SCF expression in 2,5-hexanedione-induced testicular atrophy. Biol. Reprod. 55, 185-193.(1996)
• 49) Blanchard, K.T., Allard, E.K., Boekelheide, K. (1996). Fate of germ cells in 2,5-hexanedione-induced testicular injury. I. Apoptosis is the mechanism of germ cell death. Toxicol. Appl. Pharmacol. 137, 141-148.(1996)
• 47) Johnson, K.J., Hall, E.S., Boekelheide, K. (1996). Kinesin localizes to the trans-Golgi network regardless of microtubule orientation. Eur. J. Cell Biol. 69, 276-287.(1996)
• 48) Richburg, J.H., Boekelheide, K. (1996). Mono-(2-ethylhexyl) phthalate rapidly alters both Sertoli cell vimentin filaments and germ cell apoptosis in young rat testes. Toxicol. Appl. Pharmacol. 137, 42-50.(1996)
• 50) Allard, E.K., Boekelheide, K. (1996). Fate of germ cells in 2,5-hexanedione-induced testicular injury. II. Atrophy persists due to a reduced stem cell mass and ongoing apoptosis. Toxicol. Appl. Pharmacol. 137, 149-156.(1996)
• 45) Allard, E.K., Hall, S.J., Boekelheide, K. (1995). Stem cell kinetics in rat testis after irreversible injury induced by 2,5-hexanedione. Biol. Reprod. 53, 186-192.(1995)
• 44) Hall, E.S., Hall, S.J., Boekelheide, K. (1995). 2,5-Hexanedione exposure alters microtubule motor distribution in adult rats. Fundam. Appl. Toxicol. 24, 173-182.(1995)
• 46) Redenbach, D.M., Hall, E.S., Boekelheide, K. (1995). Distribution of Sertoli cell microtubules, microtubule-dependent motors and the Golgi apparatus before and after tight junction formation in developing rat testis. Microsc. Res. Tech. 32, 504-519.(1995)
• 43) Redenbach, D.M., Boekelheide, K. (1994). Microtubules are oriented with their minus-ends directed apically before tight junction formation in rat Sertoli cells. Eur. J. Cell Biol. 65, 246-257.(1994)
• 42) Richburg, J.H., Redenbach, D.M., Boekelheide, K. (1994). Seminiferous tubule fluid secretion is a Sertoli cell microtubule-dependent process inhibited by 2,5-hexanedione exposure. Toxicol. Appl. Pharmacol. 128, 302-309.(1994)
• 40) McBurney, M.W., Staines, W.A., Boekelheide, K., Parry, D., Jardine, K., Pickavance, L. (1994). Murine PGK-1 promoter drives widespread but not uniform expression in transgenic mice. Dev. Dynamics 200, 278-293.(1994)
• 39) Redenbach, D.M., Richburg, J.H., Boekelheide, K. (1994). Microtubules with altered assembly kinetics have a decreased rate of kinesin-based transport. Cell Mot. Cytoskel. 27, 79-87.(1994)
• 41) Weist, P.M., Dong, K.L., Johnson, J.H., Tzipori, S., Boekelheide, K., Flanigan, T.P. (1994). Effect of colchicine on microtubules in Cryptosporidium parvum. J. Euk. Microbiol. 41, 66S.(1994)
• 3. Boekelheide, K. (1993). Sertoli Cell Toxicants. In The Sertoli Cell (eds. Russell, L.D., Griswold, M.D.). Cache River Press, Clearwater FL, pp. 551-575.(1993)
• 38) Boekelheide, K., Lee, J.-W., Hall, S.J., Rhind, N.R., Zaret, K.S. (1993). A tumorigenic murine Sertoli cell line that is temperature-sensitive for differentiation. Am. J. Pathol. 143, 1159-1168.(1993)
• 36) Allard, E. K., Johnson, K. J., Boekelheide, K. (1993). Colchicine disrupts the cytoskeleton of rat testis seminiferous epithelium in a stage-dependent manner. Biol. Reprod. 48, 143-153.(1993)
• 37) Johnson, K.J., Boekelheide, K. (1993). Visualization of Golgi complexes and spermatogonial cohorts of viable, intact seminiferous tubules. J. Histochem. Cytochem. 41, 299-306.(1993)
• 35) Redenbach, D.M., Boekelheide, K., Vogl, A.W. (1992). Binding between mammalian spermatid-ectoplasmic specialization complexes and microtubules. Eur. J. Cell Biol. 59, 433-448.(1992)
• 33) Boekelheide, K., Arcila, M.E., Eveleth, J. (1992). cis-Diamminedichloroplatinum (II) (cisplatin) alters microtubule assembly dynamics. Toxicol. Appl. Pharmacol. 116, 146-151.(1992)
• 31) Ashman, J.B., Hall, E.S., Eveleth, J., Boekelheide, K. (1992). Tau, the neuronal heat stable microtubule-associated protein, is also present in the cross-linked microtubule network of the testicular spermatid manchette. Biol. Reprod. 46, 120-129.(1992)
• 32) Hall, E.S., Eveleth, J., Jiang, C., Redenbach, D.M., Boekelheide, K. (1992). The distribution of the microtubule-dependent motors cytoplasmic dynein and kinesin in rat testis. Biol. Reprod. 46, 817-828.(1992)
• 34) Hall, E.S., Hall, S.J., Boekelheide, K. (1992). Sertoli cells isolated from adult 2,5-hexanedione-exposed rats exhibit atypical morphology and actin distribution. Toxicol. Appl. Pharmacol. 117, 9-18.(1992)
• 30) Hall, E.S., Eveleth, J., Boekelheide, K. (1991). 2,5-Hexanedione exposure alters the rat Sertoli cell cytoskeleton. II. Intermediate filaments and actin. Toxicol. Appl. Pharmacol. 111, 443-453.(1991)
• 28) Boekelheide, K., Hall, S.J. (1991). 2,5-Hexanedione exposure in the rat results in long-term testicular atrophy despite the presence of residual spermatogonia. J. Androl. 12, 18-26.(1991)
• 29) Johnson, K.J., Hall, E.S., Boekelheide, K. (1991). 2,5-Hexanedione exposure alters the rat Sertoli cell cytoskeleton. I. Microtubules and seminiferous tubule fluid secretion. Toxicol. Appl. Pharmacol. 111, 432-442.(1991)
• 2. Boekelheide, K., Eveleth, J., Neely, M.D., Sioussat, T.M. (1991). Microtubule assembly is altered following covalent modification by the n-hexane metabolite 2,5-hexanedione. Adv. Exp. Med. Biol. 283, 433-442.(1991)
• 26) Sioussat, T.M., Miller, F.J., Boekelheide, K. (1990). 2,5-Hexanedione-treated tubulin microinjected into sea urchin zygotes induces mitotic abnormalities. Toxicol. Appl. Pharmacol. 104, 36-46.(1990)
• 25) Boekelheide, K., Eveleth, J., Hall, S.J. (1990). Experimental cryptorchidism protects against long-term 2,5-hexanedione-induced testicular germ cell loss in the rat. J. Androl. 11, 105-112.(1990)
• 27) Neely, M.D., Erickson, H.P., Boekelheide, K. (1990). HMW-2, the Sertoli cell cytoplasmic dynein from rat testis, is a dimer composed of nearly identical subunits. J. Biol. Chem. 265, 8691-8698.(1990)
• 24) Sioussat, T., Boekelheide, K. (1989). Selection of a nucleation-promoting element following chemical modification of tubulin. Biochemistry 28, 4435-4443.(1989)
• 1. Boekelheide, K., Neely, M.D., Sioussat, T. (1989). The Sertoli cell cytoskeleton: A target for toxicant-induced germ cell loss. Toxicol. Appl. Pharmacol. 101, 373-389.(1989)
• 23) Neely, M.D., Boekelheide, K. (1988). Sertoli cell processes have axoplasmic features: an ordered microtubule distribution and an abundant high molecular weight microtubule associated protein (cytoplasmic dynein). J. Cell Biol. 107, 1767-1776.(1988)
• 22) Boekelheide, K., Anthony, D.C., Giangaspero, F., Gottfried, M.R., Graham, D.G. (1988). Aliphatic diketones: influence of dicarbonyl spacing on amine reactivity and toxicity. Chem. Res. Toxicol. 1, 200-203.(1988)
• 21) Boekelheide, K., Eveleth, J. (1988). The rate of 2,5-hexanedione intoxication, not total dose, determines the extent of testicular injury and altered microtubule assembly in the rat. Toxicol. Appl. Pharmacol. 94, 76-83.(1988)
• 19) Boekelheide, K. (1988). Rat testis during 2,5-hexanedione intoxication and recovery. I. Dose response and the reversibility of germ cell loss. Toxicol. Appl. Pharmacol. 92, 18-27.(1988)
• 20) Boekelheide, K. (1988). Rat testis during 2,5-hexanedione intoxication and recovery. II. Dynamics of pyrrole reactivity, tubulin content and microtubule assembly. Toxicol. Appl. Pharmacol. 92, 28-33.(1988)
• 18) Boekelheide, K., Eveleth, J., Tatum, A.H., Winkelman, J.W. (1987). Microtubule assembly inhibition by porphyrins and related compounds. Photochem. Photobiol. 46, 657-662.(1987)
• 16) Boekelheide, K. (1987). 2,5-Hexanedione alters microtubule assembly. I. Testicular atrophy, not nervous system toxicity, correlates with enhanced tubulin polymerization. Toxicol. Appl. Pharmacol. 88, 370-382.(1987)
• 17) Boekelheide, K. (1987). 2,5-Hexanedione alters microtubule assembly. II. Enhanced polymerization of crosslinked tubulin. Toxicol. Appl. Pharmacol. 88, 383-396.(1987)
• 15) Graham, D.G., Anthony, D.C., Szakal-Quin, Gy., Gottfried, M.R., Boekelheide, K. (1985). Covalent crosslinking of neurofilaments in the pathogenesis of n-hexane neuropathy, in The Neurofilamentous Axonopathies: The Neurotoxicology of Acrylamides, IDPN, Hexacarbons, and Carbon Disulfide (eds, Graham, D.G., Lowndes, H.E., Cranmer, J.M.). Intox Press, Little Rock.(1985)
• 14) Graham, D.G., Anthony, D.C., Szakal-Quin, Gy., Gottfried, M.R., Boekelheide, K. (1985). Covalent crosslinking of neurofilaments in the pathogenesis of n-hexane neuropathy. Neurotoxicol. 6, 55-64.(1985)
• 12) Anthony, D.C., Boekelheide, K., Graham, D.G. (1983). The effect of 3,4-dimethyl substitution on the neurotoxicity of 2,5-hexanedione. I. Accelerated clinical neuropathy is accompanied by more proximal axonal swellings. Toxicol. Appl. Pharmacol. 71, 362- 371.(1983)
• 13) Anthony, D.C., Boekelheide, K., Anderson, C.W., Graham, D.G. (1983). The effect of 3,4-dimethyl substitution on the neurotoxicity of 2,5-hexanedione. II. Dimethyl substitution accelerates pyrrole formation and protein crosslinking. Toxicol. Appl. Pharmacol. 71, 372-382.(1983)
• 11) Graham, D.G., Anthony, D.C., Boekelheide, K. (1982). In vitro and in vivo studies of the molecular pathogenesis of n-hexane neuropathy. Neurobehav. Toxicol. Teratol. 4, 629-634.(1982)
• 10) Blumenkopf, B., Boekelheide, K. (1982). Neck paraganglioma with a pituitary ademona. J. Neurosurg. 57, 426-429.(1982)
• 9) Graham, D.G., Anthony, D.C., Boekelheide, K., Maschmann, N.A., Richards, R.G., Wolfram, J.W., Shaw, B.R. (1982). Studies of the molecular pathogenesis of hexane neuropathy. II. Evidence that the pyrrole derivatization of lysyl residues leads to protein crosslinking. Toxicol. Appl. Pharmacol. 64, 415-422.(1982)
• 8) Mize, P.D., Jeffs, P.W., Boekelheide, K. (1980). Structure determination of the active sulfhydryl reagent in gill tissue of the mushroom Agaricus bisporus. J. Org. Chem. 45, 3540-3543.(1980)
• 6) 4. Boekelheide, K., Graham, D.G., Mize, P.D., Koo, E.H. (1980). Melanocytotoxicity and the mechanism of activation of -L-glutaminyl-4-hydroxybenzene. J. Invest. Dermatol. 75, 322-327.(1980)
• 5) Boekelheide, K., Graham, D.G., Mize, P.D., Jeffs, P.W. (1980). The metabolic pathway catalyzed by the tyrosinase of Agaricus bisporus. J. Biol. Chem. 255, 4766-4771.(1980)
• 7) Boekelheide, K., Graham, D.G., Mize, P.D., Vogel, F.S. (1980). The role of -glutamyl transpeptidase in the nephrotoxicity of an Agaricus bisporus metabolite. Am. J. Pathol. 100, 651-662.(1980)
• 4) Boekelheide, K., Graham, D.G., Mize, P.D., Anderson, C.W., Jeffs, P.W. (1979). Synthesis of -L-glutaminyl-[3,5-3H]4-hydroxybenzene and the study of reactions catalyzed by the tyrosinase of Agaricus bisporus. J. Biol. Chem. 254, 12185-12191.(1979)
• 3) Boekelheide, K., Patt, S.L., Weisz, G., Baldo, J.H., Sykes, B.D. (1979) Lysozyme catalysis: kinetics of the hydrolysis of cell wall oligosaccharides. Can. J. Biochem. 57, 785-88.(1979)
• 2) Vogel, F.S., Kemper, L.A.K., Boekelheide, K., Graham, D.G., Jeffs, P.W. (1979). Intracellular activation of -L-glutaminyl-4-hydroxybenzene by tyrosinase, a mechansim for selective cytotoxicity against melanocarcinoma. Cancer Res. 39, 1490-1493.(1979)
• 1) Patt, S.L., Baldo, J.H., Boekelheide, K., Weisz, G., Sykes, B.D. (1978). The nuclear magnetic resonance determination of the conformation of saccharides bound in subsite D of lysozyme. Can. J. Biochem. 56, 624-9.(1978)