The research program of the lab involves understanding the fundamental structure and function relationships of RNases H with the long term goals of a) unmasking novel nucleic acid metabolic functions associated with the enzymes and b) understanding their placement in the replicative life cycle of the pathogenic protozoan Trypanosoma brucei. Trypanosomes are amongst the earliest branching eukaryotes and are the models in which RNA editing and trans-splicing were initially discovered. Accordingly, they are well suited for our studies.

Institutions

BU

Research Description

The research program of the lab involves understanding the fundamental structure and function relationships of RNases H with the long term goals of a) unmasking novel nucleic acid metabolic functions associated with the enzymes and b) understanding their placement in the replicative life cycle of the pathogenic protozoan Trypanosoma brucei. Trypanosomes are amongst the earliest branching eukaryotes and are the models in which RNA editing and trans-splicing were initially discovered. Accordingly, they are well suited for our studies.

Viral RNases H are also being evaluated as potential therapeutic targets, and our studies emphasize identifying RNase H mutations which may account for drug resistance during the course of drug treatment. RNases H are widely distributed nucleic acid metabolic enzymes which degrade the RNA strand of RNA:DNA heteroduplexes. They are also utilized by all biological systems. Our current models are excellent platforms from which to move and study these enzymes in related systems.

Awards

- Invited Participant, Course on Vectors and Vector Borne Diseases, Mali, W. Africa, 1997
- NSF Career Development Award, 1997
- Lifespan/Brown/Tufts University Center for AIDS Research Developmental Grant, 1998
- Lifespan/Brown/Tufts University Center for AIDS Research Developmental Grant, 1999
- School of Medicine Dean's Teaching Excellence Award, 2001
- American Foundation for AIDS Research (amFAR) Investigator Grant, 2001
- School of Medicine Dean's Teaching Excellence Award, 2002

Affiliations

- American Association for the Advancement of Science
- American Society for Tropical Medicine and Hygiene
- American Society for Cell Biology
- New England Association of Parasitologists

Funded Research

N/A

Courses Taught

• Biology of Emerging Microbial Diseases (BIO 54)

Selected Publications

• 1. Tong R. Wu, Y. Kate Hong, Xu-Dong Wang, Mike Y. Ling, Ana M. Dragoi, Alicia S. Chung, Andrew G. Campbell, Zhi-Yong Han, Gen-Sheng Feng, and Y. Eugene Chin (2002) SHP-2 is a dual-specificity phosphatase involved in Stat1 dephosphorylation at both tyrosine and serine residues in nuclei J. Biol. Chem. 277 : 49, 47572 - 47580(2002)
• 2. A. G. Campbell (2001) Expression of Moloney Murine Leukemia Virus Ribonuclease H Rescues The Growth Defect of an Escherichia coli Mutant., J. Virology 75 :13 6212 -6217(2001)
• 3. Kobil, JH, Campbell AG. (2000) Functional Analysis of the Domain Organization of Trypanosoma brucei RNase HI Biochem. Biophys. Res. Comm. 270: 336 - 342(2000)
• 4. Kobil, JH, Campbell AG (2000) Trypanosoma brucei RNase HI Requires its Divergent Spacer Subdomain for Enzymatic Function And its RNA Binding Motif for Nuclear Localization Molec. Biochem. Parasitol.107: 135 - 42(2000)
• 6. Campbell, AG, J.H. Kobil, JH (1999) Functional Analysis of Trypanosoma brucei RNase HI : Separable Nuclear targeting/RNA Binding and Ribonuclease H Subdomains. Molec. Biol. of the Cell. Suppl. 10: p439a (Abst.)(1999)
• 5. Pearson, B, Campbell AG (1999) Molecular Cloning of the Trypanosoma brucei Class II Ribonuclease H _-subunit Molec. Biol. of the Cell. Suppl. 10: p281a (Abst.)(1999)
• 7. Hesslein, DGT ,Campbell AG (1997) Molecular Cloning and Expression of a Ribonuclease H from the Kinetoplastid, Trypanosoma brucei. Molec. Biochem. Parasitol. 86 : 121 - 126(1997)
• 8. Campbell, AG. (1996) Studies of Kinetoplastid Ribonucleases H 4th International Ribonucleases H Meeting. Ocean City, MD. USA(1996)
• 9. Akkina, RK, Rosenblatt, JD, Campbell, AG, Chen, ISY , JA Zack (1994). Blood 84 : 1393 -1398 Modeling Human Lymphoid Precursor Cell Gene Therapy in the SCID-hu Mouse(1994)
• 10. Campbell, AG, DS Ray (1993) Functional Complementation of an Escherichia coli. Ribonuclease H Mutation by a cloned genomic fragment from the trypanosomatid, Crithidia fasciculata. Proc. Natl. Acad. Sci. USA 90 : 9350 - 9354(1993)
• 11. Olsen, P., Fessler, LI., Nelson, R., Sterne, RE., Campbell, AG., and Fessler, J.H. (1990) Glutactin, a novel Drosophila basement membrane related glycoprotein with sequence similarity to serine esterases. EMBO. J. 9 : 1219 -1227(1990)
• 12. Fessler, JH., Blumberg, B., Campbell, AG., Garrison, K.,Mackrell, AJ., Olsen, PF. and Fessler,LI. (1989) Connectors of Supramolecular Assemblies. In 'Cytoskeletal and Extracellular Proteins. Structure Interactions and Assembly. Aebi, U., and Engel, J. eds. 2nd International EBSA Symposium. Springer-Verlag(1989)
• 15. Fessler, LI., Campbell, AG., Blumberg, B., Mackrell, A.,and Fessler, JH. (1987) Basement membrane synthesis in Drosophila embryos. J. Cell. Biochem. 11A: 287(1987)
• 14. Fessler, LI., Campbell, AG., Duncan, KG., and Fessler, JH. (1987) Drosophila Laminin: Characterization and localization. J. Cell. Biol.105 : 2383 – 2391(1987)
• 13. Campbell, AG., Fessler , LI., and Fessler, JH. (1987) Papilin: A Drosophila proteoglycan-like sulfated glycoprotein from basement membranes. J. Biol .Chem. 262 : 17605 - 17612(1987)
• 17. Fessler. L I., Campbell, AG., Blumberg, B., and Fessler, JH. (1986). J. Cell. Biochem. Suppl. 10D : 22(1986)
• 16. Fessler, JH., Lunstrum, G., Duncan, KG., Campbell, AG., Sterne, RE. and Fessler, LI. (1984) Evolutionary constancy of basement membrane components. In 42nd Symposium on Developmental Biology (R. Trelstad, ed), pp. 207 - 219(1984)
• 18. Young, P., and Campbell, AG. (1982) The synthesis of a dipeptide from its component amino acids. J. Chemical Education 59 : 701 – 702(1982)