Faculty Directory

Office of the Dean Administration Departments Research Alpert Medical School Giving

Alexander Brodsky, PhD

Edit My Page
Alexander Brodsky

Title: Assistant Professor of Medical Science
Department: Molecular Biology, Cell Biology, & Biochemistry

Alexander_Brodsky@brown.edu

Download Alexander Brodsky's Curriculum Vitae in PDF Format

 
Overview | Research | Grants/Awards | Publications

We are characterizing the key differences between ovarian primary and metastatic tumors. This has led to the discovery of new microRNAs and pathways important for ovarian cancer cell survival and response to chemotherapy.

We have identified a key feedback loop important for the regulation of cholesterol that mediates ovarian and breast cancer growth and survival. We are investigating the mechanisms of this regulation and translating our discoveries to develop novel anti-tumor drug combinations.

Biography

Dr. Brodsky received his BA from the University of Pennsylvania where he worked with Dr. Ponzy Lu exploring DNA dynamics during electrophoresis. Dr. Brodsky recieved his PhD from MIT working with Dr. James R. Williamson where he developed a structural model of the HIV tat-TAR protein-RNA interaction using NMR spectroscopy.

Dr. Brodsky received postdoctoral training from Dr. Pamela A. Silver at the Dana-Farber Cancer Institute/Harvard Medical School where he discovered mechanisms of mRNA export. He was among the first to use chromatin immunoprecipitation combined with genomic tiling arrays to define the coupling of transcription and mRNA processing.

Dr. Brodsky joined the MCB department and the Center of Genetics and Proteomics at Brown University in 2005.

Research Description

Cancer genomics and systems biology

We are exploring the role of RNA processing in response to hormones such as estrogen in breast cancer. We are identifying novel components mediating hormone control of cancer cells that are regulated post-transcriptionally.

We are also probing hormone combinations mediating cancer cell growth and death. We are testing how these regulatory networks affect cancer cells in mouse models and in genomic data obtained from patientsWe are characterizing the key differences between ovarian primary and metastatic tumors. This has led to the discovery of new microRNAs and pathways important for ovarian cancer cell survival and response to chemotherapy.

We have identified a key feedback loop important for the regulation of cholesterol that mediates ovarian and breast cancer growth and survival. We are investigating the mechanisms of this regulation and translating our discoveries to develop novel anti-tumor drug combinations.

Awards

NHGRI K22 Genome Scholar Career Award
Ellison Medical Foundation Scholar

Affiliations

American Association for the Advancement of Science
RNA Society

Funded Research

Mary Kay Foundation (PI)

Ellison Medical Foundation (PI) (completed)
Susan G. Komen Breast Cancer Foundation (co-PI) (completed)
ARRA Supplement: Biocurrents Research Center (PI subcontract) (completed)

DOD/CDMRP Breast Cancer Synergy Award(Completed)
NHGRI K22 Genome Scholar Career Development Award (Completed)

Selected Publications

  • 16. Neretti, N., Wang, P.Y., Brodsky, A.S., Nyguyen, H.H., White, K.P., Rogina, B., and Helfand, S.L. (2009). Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage. Proc Natl Acad Sci U S A 106: 2277-2282 (PMID: 19164521).(2009)
  • 15. McKee, A.E., Neretti, N., Carvalho, L.E., Meyer, C.A., Fox, E.A., Brodsky, A.S.*, and Silver, P.A. (2007). Exon expression profiling reveals stimulus-mediated exon use in neural cells. Genome Biol 8: R159 (PMID: 17683528).(2007)
  • 14. Swinburne, I.A., Meyer, C.A., Liu, X.S., Silver, P.A., and Brodsky, A.S.* (2006). Genomic localization of RNA binding proteins reveals links between pre-mRNA processing and transcription. Genome Res 16: 912-921 (PMID: 16769980).(2006)
  • 13. Gama-Carvalho, M., Barbosa-Morais, N.L., Brodsky, A.S., Silver, P.A., and Carmo-Fonseca, M. (2006). Genome-wide identification of functionally distinct subsets of cellular mRNAs associated with two nucleocytoplasmic-shuttling mammalian splicing factors. Genome Biol 7: R113 (PMID: 17137510).(2006)
  • 12. Carroll, J.S., Meyer, C.A., Song, J., Li, W., Geistlinger, T.R., Eeckhoute, J., Brodsky, A.S., Keeton, E.K., Fertuck, K.C., Hall, G.F., Wang, Q., Bekiranov, S., Sementchenko, V., Fox, E.A., Silver, P.A., Gingeras, T.R., Liu, X.S., and Brown, M. (2006). Genome-wide analysis of estrogen receptor binding sites. Nat Genet 38: 1289-1297 (PMID: 17013392).(2006)
  • 11. Carroll, J.S., Liu, X.S., Brodsky, A.S., Li, W., Meyer, C.A., Szary, A.J., Eeckhoute, J., Shao, W., Hestermann, E.V., Geistlinger, T.R., Fox, E.A., Silver, P.A., and Brown, M. (2005). Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell 122: 33-43 (PMID: 16009131).(2005)
  • 10. Brodsky, A.S.*, Meyer, C.A., Swinburne, I.A., Hall, G., Keenan, B.J., Liu, X.S., Fox, E.A., and Silver, P.A. (2005). Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells. Genome Biol 6: R64 (PMID: 16086846).(2005)
  • 9. Brodsky, A.S., Johnston, A.P., Trau, M., and Silver, P.A. (2003). Analysis of RNA-protein interactions by flow cytometry. Curr Opin Mol Ther 5: 235-240 (PMID: 12870432).(2003)
  • 7. Brodsky, A.S., and Silver, P.A. (2002). Identifying proteins that affect mRNA localization in living cells. Methods 26: 151-155 (PMID: 12054891).(2002)
  • 6. Brodsky, A.S.*, and Silver, P.A. (2002). A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry. Mol Cell Proteomics 1: 922-929 (PMID: 12543929).(2002)
  • 8. Dayie, K.T., Brodsky, A.S., and Williamson, J.R. (2002). Base flexibility in HIV-2 TAR RNA mapped by solution (15)N, (13)C NMR relaxation. J Mol Biol 317: 263-278 (PMID: 11902842).(2002)
  • 5. Hennig, M., Williamson, J.R., Brodsky, A.S., and Battiste, J.L. (2001). Recent advances in RNA structure determination by NMR. Curr Protoc Nucleic Acid Chem Chapter 7: Unit 7.7 (PMID: 18428875).(2001)
  • 4. Brodsky, A.S., and Silver, P.A. (2000). Pre-mRNA processing factors are required for nuclear export. RNA 6: 1737-1749 (PMID: 11142374).(2000)
  • 3. Brodsky, A.S., and Silver, P.A. (1999). Nuclear transport HEATs up. Nat Cell Biol 1: E66-67 (PMID: 10559913).(1999)
  • 2. Brodsky, A.S., Erlacher, H.A., and Williamson, J.R. (1998). NMR evidence for a base triple in the HIV-2 TAR C-G.C+ mutant-argininamide complex. Nucleic Acids Res 26: 1991-1995 (PMID: 9518494).(1998)
  • 1. Brodsky, A.S., and Williamson, J.R. (1997). Solution structure of the HIV-2 TAR-argininamide complex. J Mol Biol 267: 624-639 (PMID: 9126842).(1997)