1999 Syllabus

Pfizer Bio154A (Molecular Genetics)

Instructor: Dr. E. A. Hendrickson JWW Rm 162 863-3667 e-mail: Eric_Hendrickson@brown.edu

TA: Robyn Tanny JWW Rm 110 863-1726 e-mail: Robyn_Tanny@brown.edu

TA: Stephen Dunaway JWW Rm 110 863-1726 e-mail: Stephen_Dunaway@brown.edu

TA: Xiaolan Hu JWW Rm 110 863-1726 e-mail: Xiaolan_Hu@brown.edu

REQUIRED TEXTS: Recombinant DNA (2nd Edition), Watson et al., (1992), W. H. Freeman & Co.

Hannah's Heirs, Daniel Pollen, (1993), Oxford Press

Racing to the Beginning of the Road, Robert Weinberg, (1996), W. H. Freeman & Co.

I

1. Sep. 8

Introduction

Chapter 1 Establishing the role of genes within cells

Chapter 2 DNA is the primary genetic material

Chapter 3 Elucidation of the genetic code

Chapter 4 The genetic elements that control gene expression

2. Sep. 15

Chapter 8 The complexity of the genome

Chapter 9 Controlling eukaryotic gene expression

Chapter 5 Methods of creating recombinant DNA molecules-"cloning"

Chapter 7 The isolation of cloned genes-1: Immunoscreening (Hodgkin's Disease)

------------- PROBLEM SET I HANDED OUT

3. Sep. 22

Chapter 7 The isolation of cloned genes-2: Panning (Hodgkin's Disease/CD75)

Chapter 7 The isolation of cloned genes-3: Functional complementation I (Fanconi's Anaemia)

------------- PROBLEM SET I DUE BACK

------------- PROBLEM SET II HANDED OUT

4. Sep. 29

Chapter 6 The polymerase chain reaction (PCR): cCcancer

Chapter 11 In vitro mutagenesis-1: HIV tat

------------- PROBLEM SET II DUE BACK

------------- TAKE HOME EXAM I HANDED OUT

II

5. Oct. 6

Chapter 11 In vitro mutagenesis-2: HIV tat

Chapter 12 Transferring genes into mammalian cells-1: Oct-3

Chapter 13 Using yeast to study eukaryotic gene function-1: Complementation (cyclin)

------------- TAKE HOME EXAM I DUE BACK

------------- PROBLEM SET III HANDED OUT

6. Oct. 13

Chapter 13 Using yeast to study eukaryotic gene function-2: Complementation/two hybrid system

Chapter 16 Molecules of immune recognition-1: V(D)J recombination

Chapter 16 Molecules of immune recognition-2: RAG-1

------------- PROBLEM SET III DUE BACK

7. Oct. 20

Chapter 16 Molecules of immune recognition-3: RAG-1/RAG-2

------------- Sex determination-1: ZFY

------------- Sex determination-2: SXR

------------- PROBLEM SET IV HANDED OUT

Oct. 27 NO CLASS

8. Nov. 3

------------- Sex determination-3: SXR

Chapter 14 The introduction of foreign genes into mice-1: Transgenics (SXR, Inv)

------------- PROBLEM SET IV DUE BACK

------------- TAKE HOME EXAM II HANDED OUT

III

9. Nov. 10

Chapter 14 The introduction of foreign genes into mice-2: Knockouts (RAG-2, CaMK)

Chapter 14 The introduction of foreign genes into mice-3: Conditional Knockouts (b-pol)

------------- TAKE HOME EXAM II DUE BACK

10. Nov. 17

Chapter 29 Studying whole genomes: YACS

Chapter 26 Mapping and cloning human disease genes-1: Fragile X

------------- PROBLEM SET V HANDED OUT

11. Nov. 24

Chapter 26 Mapping and cloning human disease genes-2: HD, BRCA-1/2

------------- PROBLEM SET V DUE BACK

12. Dec. 1

Chapter 26 Mapping and cloning human disease genes-3: Ob, AD

Chapter 28 Working toward human gene therapy: CF

------------- PROBLEM SET VI HANDED OUT

13. Dec. 8

Chapter 30 The human genome initiative-Finding all the human genes

------------- PROBLEM SET VI DUE BACK

14. Dec. 15

FINAL EXAM

 

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